Detailed information of TA system TA100622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4425588..4426190
• Replicon: chromosome
• Accession: NZ_CP028122
• Organism: Escherichia coli O43 str. RM10042

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I3W_RS24230
• Protein ID: WP_000897305.1
• Coordinates: 4425588..4425899 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I3W_RS24235
• Protein ID: WP_000356397.1
• Coordinates: 4425900..4426190 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3W_RS24205	4421502..4422101	+	600	WP_001295269.1	glucose-1-phosphatase	-
I3W_RS24210	4422095..4422967	+	873	WP_000920762.1	virulence factor BrkB family protein	-
I3W_RS24215	4422964..4423401	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I3W_RS24220	4423446..4424387	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I3W_RS24225	4424451..4425359	-	909	WP_001162704.1	alpha/beta hydrolase	-
I3W_RS24230	4425588..4425899	+	312	WP_000897305.1	hypothetical protein	Toxin
I3W_RS24235	4425900..4426190	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I3W_RS24245	4426795..4427013	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
I3W_RS24250	4427232..4427474	+	243	WP_001086388.1	hypothetical protein	-
I3W_RS24255	4427804..4428733	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
I3W_RS24260	4428730..4429365	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I3W_RS24265	4429362..4430264	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T100622 WP_000897305.1 NZ_CP028122:4425588-4425899 [Escherichia coli O43 str. RM10042]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T100622 NZ_CP028122:4425588-4425899 [Escherichia coli O43 str. RM10042]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT100622 WP_000356397.1 NZ_CP028122:4425900-4426190 [Escherichia coli O43 str. RM10042]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT100622 NZ_CP028122:4425900-4426190 [Escherichia coli O43 str. RM10042]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7