Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2313341..2313843 Replicon chromosome
Accession NZ_CP028122
Organism Escherichia coli O43 str. RM10042

Toxin (Protein)


Gene name yoeB Uniprot ID D8A584
Locus tag I3W_RS13460 Protein ID WP_000767828.1
Coordinates 2313341..2313595 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I3W_RS13465 Protein ID WP_001259255.1
Coordinates 2313592..2313843 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3W_RS13430 2308356..2308583 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
I3W_RS13435 2308597..2309655 - 1059 WP_000492344.1 transport protein YeeE -
I3W_RS13440 2309834..2311192 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
I3W_RS13445 2311182..2311244 - 63 WP_010723108.1 membrane protein YoeI -
I3W_RS13450 2311459..2312388 - 930 WP_000803351.1 LysR family transcriptional regulator -
I3W_RS13455 2312434..2313258 - 825 WP_000754758.1 SDR family oxidoreductase -
I3W_RS13460 2313341..2313595 - 255 WP_000767828.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I3W_RS13465 2313592..2313843 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I3W_RS13470 2314126..2314176 + 51 WP_001364200.1 his operon leader peptide -
I3W_RS13480 2314322..2315221 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
I3W_RS13485 2315227..2316531 + 1305 WP_032241630.1 histidinol dehydrogenase -
I3W_RS13490 2316528..2317598 + 1071 WP_032241632.1 histidinol-phosphate transaminase -
I3W_RS13495 2317598..2318665 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.56 Da        Isoelectric Point: 8.0353

>T100616 WP_000767828.1 NZ_CP028122:c2313595-2313341 [Escherichia coli O43 str. RM10042]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T100616 NZ_CP028122:c2313595-2313341 [Escherichia coli O43 str. RM10042]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT100616 WP_001259255.1 NZ_CP028122:c2313843-2313592 [Escherichia coli O43 str. RM10042]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT100616 NZ_CP028122:c2313843-2313592 [Escherichia coli O43 str. RM10042]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G465

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References