Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 280366..280907 Replicon chromosome
Accession NZ_CP028122
Organism Escherichia coli O43 str. RM10042

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag I3W_RS02560 Protein ID WP_000615976.1
Coordinates 280366..280644 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag I3W_RS02565 Protein ID WP_000729704.1
Coordinates 280647..280907 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3W_RS02545 277948..278526 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
I3W_RS02550 278732..279499 + 768 WP_000333380.1 class II glutamine amidotransferase -
I3W_RS02555 279470..280210 - 741 WP_001225679.1 murein L,D-transpeptidase -
I3W_RS02560 280366..280644 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
I3W_RS02565 280647..280907 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
I3W_RS02570 281093..281866 + 774 WP_000543897.1 C40 family peptidase -
I3W_RS02575 281923..282279 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
I3W_RS02580 282272..282550 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
I3W_RS02585 282655..284367 - 1713 Protein_249 flagellar biosynthesis protein FlhA -
I3W_RS02590 284339..285124 + 786 WP_000207589.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T100596 WP_000615976.1 NZ_CP028122:c280644-280366 [Escherichia coli O43 str. RM10042]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T100596 NZ_CP028122:c280644-280366 [Escherichia coli O43 str. RM10042]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT100596 WP_000729704.1 NZ_CP028122:c280907-280647 [Escherichia coli O43 str. RM10042]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT100596 NZ_CP028122:c280907-280647 [Escherichia coli O43 str. RM10042]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References