Detailed information of TA system TA100591

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 58821..59074
• Replicon: plasmid pRM10042-2
• Accession: NZ_CP028121
• Organism: Escherichia coli O43 str. RM10042

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: I3W_RS00890
• Protein ID: WP_001336447.1
• Coordinates: 58821..58970 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	59018..59074 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3W_RS00855	55913..56152	-	240	WP_071988618.1	MarR family transcriptional regulator	-
I3W_RS00860	56165..56381	-	217	Protein_47	hypothetical protein	-
I3W_RS00875	57128..57985	-	858	WP_047087087.1	incFII family plasmid replication initiator RepA	-
I3W_RS00880	57978..58052	-	75	WP_001365705.1	RepA leader peptide Tap	-
I3W_RS00885	58277..58537	-	261	WP_000083819.1	replication regulatory protein RepA	-
I3W_RS00890	58821..58970	-	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	59018..59074	+	57	NuclAT_0	-	Antitoxin
-	59018..59074	+	57	NuclAT_0	-	Antitoxin
-	59018..59074	+	57	NuclAT_0	-	Antitoxin
-	59018..59074	+	57	NuclAT_0	-	Antitoxin
I3W_RS00905	59773..60234	-	462	WP_108942208.1	thermonuclease family protein	-
I3W_RS00910	61019..62533	-	1515	WP_108942200.1	IS66 family transposase	-
I3W_RS00915	62583..62930	-	348	WP_000612591.1	IS66 family insertion sequence element accessory protein TnpB	-
I3W_RS00920	62927..63307	-	381	WP_001171532.1	IS66 family insertion sequence hypothetical protein	-
I3W_RS00925	63667..63816	+	150	WP_024199253.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	espP / espP / espP / hlyD / hlyB / hlyB / hlyA / hlyC	1..109466	109466

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T100591 WP_001336447.1 NZ_CP028121:c58970-58821 [Escherichia coli O43 str. RM10042]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T100591 NZ_CP028121:c58970-58821 [Escherichia coli O43 str. RM10042]
ATGACGAAATATACCCTTATTGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT100591 NZ_CP028121:59018-59074 [Escherichia coli O43 str. RM10042]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin