Detailed information of TA system TA100586

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 5996..6422
• Replicon: plasmid pRM9322-1
• Accession: NZ_CP028118
• Organism: Escherichia coli O111 str. RM9322

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: I3O_RS27595
• Protein ID: WP_001312861.1
• Coordinates: 5996..6154 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	6198..6422 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3O_RS27555	1366..2055	-	690	WP_000283394.1	conjugal transfer transcriptional regulator TraJ	-
I3O_RS27560	2242..2625	-	384	WP_001151524.1	relaxosome protein TraM	-
I3O_RS27565	3039..3548	+	510	Protein_6	transglycosylase SLT domain-containing protein	-
I3O_RS27570	3845..4666	-	822	WP_001234469.1	DUF945 domain-containing protein	-
I3O_RS27575	4787..5074	-	288	WP_000107535.1	hypothetical protein	-
I3O_RS27595	5996..6154	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	6198..6422	-	225	NuclAT_0	-	Antitoxin
-	6198..6422	-	225	NuclAT_0	-	Antitoxin
-	6198..6422	-	225	NuclAT_0	-	Antitoxin
-	6198..6422	-	225	NuclAT_0	-	Antitoxin
I3O_RS28440	6234..6422	+	189	WP_001299721.1	hypothetical protein	-
I3O_RS27600	6434..7153	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
I3O_RS27605	7150..7584	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
I3O_RS27610	7639..9597	-	1959	WP_001145472.1	ParB/RepB/Spo0J family partition protein	-
I3O_RS27615	9656..9889	-	234	WP_000006004.1	DUF905 domain-containing protein	-
I3O_RS27620	9945..10472	-	528	WP_000290792.1	single-stranded DNA-binding protein	-
I3O_RS28445	10945..11241	-	297	WP_077694982.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / hlyD / hlyB / hlyA / hlyC	1..78469	78469

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T100586 WP_001312861.1 NZ_CP028118:c6154-5996 [Escherichia coli O111 str. RM9322]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T100586 NZ_CP028118:c6154-5996 [Escherichia coli O111 str. RM9322]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT100586 NZ_CP028118:c6422-6198 [Escherichia coli O111 str. RM9322]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin