Detailed information of TA system TA100582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4586903..4587505
• Replicon: chromosome
• Accession: NZ_CP028117
• Organism: Escherichia coli O111 str. RM9322

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I3O_RS24360
• Protein ID: WP_000897305.1
• Coordinates: 4586903..4587214 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I3O_RS24365
• Protein ID: WP_000356397.1
• Coordinates: 4587215..4587505 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3O_RS24330	4581933..4582718	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
I3O_RS24335	4582817..4583416	+	600	WP_001315111.1	glucose-1-phosphatase	-
I3O_RS24340	4583410..4584282	+	873	WP_000920762.1	virulence factor BrkB family protein	-
I3O_RS24345	4584279..4584716	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I3O_RS24350	4584761..4585702	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I3O_RS24355	4585766..4586674	-	909	WP_001162704.1	alpha/beta hydrolase	-
I3O_RS24360	4586903..4587214	+	312	WP_000897305.1	hypothetical protein	Toxin
I3O_RS24365	4587215..4587505	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I3O_RS24375	4588110..4588328	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
I3O_RS24380	4588547..4588789	+	243	WP_001086388.1	hypothetical protein	-
I3O_RS24385	4589120..4590049	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
I3O_RS24390	4590046..4590681	-	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
I3O_RS24395	4590678..4591580	-	903	WP_000331369.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T100582 WP_000897305.1 NZ_CP028117:4586903-4587214 [Escherichia coli O111 str. RM9322]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T100582 NZ_CP028117:4586903-4587214 [Escherichia coli O111 str. RM9322]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT100582 WP_000356397.1 NZ_CP028117:4587215-4587505 [Escherichia coli O111 str. RM9322]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT100582 NZ_CP028117:4587215-4587505 [Escherichia coli O111 str. RM9322]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7