Detailed information of TA system TA100578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3235139..3235866
• Replicon: chromosome
• Accession: NZ_CP028117
• Organism: Escherichia coli O111 str. RM9322

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: I3O_RS17385
• Protein ID: WP_000547559.1
• Coordinates: 3235555..3235866 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I3O_RS17380
• Protein ID: WP_000126294.1
• Coordinates: 3235139..3235558 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3O_RS17360	3230189..3230716	-	528	WP_001078781.1	electron transport protein HydN	-
I3O_RS17365	3230865..3231875	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
I3O_RS17370	3232135..3233592	+	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
I3O_RS17375	3233601..3235025	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
I3O_RS17380	3235139..3235558	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
I3O_RS17385	3235555..3235866	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
I3O_RS17390	3236040..3236783	-	744	WP_001369451.1	hypothetical protein	-
I3O_RS17395	3236826..3237296	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
I3O_RS17400	3237289..3237699	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
I3O_RS17405	3237696..3238463	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
I3O_RS17410	3238463..3239005	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
I3O_RS17415	3239015..3240724	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T100578 WP_000547559.1 NZ_CP028117:c3235866-3235555 [Escherichia coli O111 str. RM9322]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T100578 NZ_CP028117:c3235866-3235555 [Escherichia coli O111 str. RM9322]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT100578 WP_000126294.1 NZ_CP028117:c3235558-3235139 [Escherichia coli O111 str. RM9322]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT100578 NZ_CP028117:c3235558-3235139 [Escherichia coli O111 str. RM9322]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163