Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2485462..2485964 Replicon chromosome
Accession NZ_CP028117
Organism Escherichia coli O111 str. RM9322

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag I3O_RS13555 Protein ID WP_000767829.1
Coordinates 2485462..2485716 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I3O_RS13560 Protein ID WP_001259255.1
Coordinates 2485713..2485964 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3O_RS13525 2480477..2480704 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
I3O_RS13530 2480718..2481776 - 1059 WP_000492340.1 transport protein YeeE -
I3O_RS13535 2481955..2483313 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
I3O_RS13540 2483303..2483365 - 63 WP_010723108.1 membrane protein YoeI -
I3O_RS13545 2483580..2484509 - 930 WP_000803331.1 LysR family transcriptional regulator -
I3O_RS13550 2484555..2485379 - 825 WP_000754755.1 SDR family oxidoreductase -
I3O_RS13555 2485462..2485716 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I3O_RS13560 2485713..2485964 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I3O_RS13565 2486248..2486298 + 51 WP_001364200.1 his operon leader peptide -
I3O_RS13575 2486444..2487343 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
I3O_RS13580 2487349..2488653 + 1305 WP_001345258.1 histidinol dehydrogenase -
I3O_RS13585 2488650..2489729 + 1080 WP_000102292.1 histidinol-phosphate transaminase -
I3O_RS13590 2489726..2490793 + 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T100576 WP_000767829.1 NZ_CP028117:c2485716-2485462 [Escherichia coli O111 str. RM9322]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T100576 NZ_CP028117:c2485716-2485462 [Escherichia coli O111 str. RM9322]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT100576 WP_001259255.1 NZ_CP028117:c2485964-2485713 [Escherichia coli O111 str. RM9322]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT100576 NZ_CP028117:c2485964-2485713 [Escherichia coli O111 str. RM9322]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References