Detailed information of TA system TA100562

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1657206..1657577
• Replicon: chromosome
• Accession: NZ_CP028117
• Organism: Escherichia coli O111 str. RM9322

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: I3O_RS08840
• Protein ID: WP_001443846.1
• Coordinates: 1657428..1657577 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1657206..1657384 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3O_RS08815	1653161..1654534	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
I3O_RS08820	1654663..1655598	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
I3O_RS08825	1655650..1656885	-	1236	WP_000040839.1	site-specific integrase	-
I3O_RS08830	1656887..1657102	-	216	WP_000079604.1	excisionase XisR	-
I3O_RS08835	1657202..1657390	-	189	WP_001302840.1	DUF1187 family protein	-
-	1657206..1657384	+	179	NuclAT_0	-	Antitoxin
-	1657206..1657384	+	179	NuclAT_0	-	Antitoxin
-	1657206..1657384	+	179	NuclAT_0	-	Antitoxin
-	1657206..1657384	+	179	NuclAT_0	-	Antitoxin
I3O_RS08840	1657428..1657577	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
I3O_RS08845	1657633..1658442	-	810	WP_000166313.1	recombination protein RecT	-
I3O_RS08850	1658435..1661035	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
I3O_RS08855	1661137..1661412	-	276	WP_001344816.1	hypothetical protein	-
I3O_RS08860	1661487..1661657	-	171	WP_001352098.1	conserved protein, Rac prophage	-
I3O_RS08865	1661657..1661878	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espM1 / paa / nleG7'	1655650..1715360	59710

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T100562 WP_001443846.1 NZ_CP028117:c1657577-1657428 [Escherichia coli O111 str. RM9322]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T100562 NZ_CP028117:c1657577-1657428 [Escherichia coli O111 str. RM9322]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT100562 NZ_CP028117:1657206-1657384 [Escherichia coli O111 str. RM9322]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin