Detailed information of TA system TA100551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4665893..4666495
• Replicon: chromosome
• Accession: NZ_CP028116
• Organism: Escherichia coli O26 str. RM8426

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I3M_RS26120
• Protein ID: WP_000897305.1
• Coordinates: 4666184..4666495 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I3M_RS26115
• Protein ID: WP_000356397.1
• Coordinates: 4665893..4666183 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3M_RS26080	4661439..4662074	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
I3M_RS26085	4662071..4663000	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
I3M_RS26095	4663400..4664380	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
I3M_RS26100	4664609..4664851	-	243	WP_001086390.1	hypothetical protein	-
I3M_RS26105	4665070..4665288	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
I3M_RS26115	4665893..4666183	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I3M_RS26120	4666184..4666495	-	312	WP_000897305.1	hypothetical protein	Toxin
I3M_RS26125	4666724..4667632	+	909	WP_001162704.1	alpha/beta hydrolase	-
I3M_RS26130	4667696..4668637	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I3M_RS26135	4668682..4669119	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I3M_RS26140	4669116..4669988	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I3M_RS26145	4669982..4670581	-	600	WP_001315111.1	glucose-1-phosphatase	-
I3M_RS26150	4670680..4671465	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4663400..4664380	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T100551 WP_000897305.1 NZ_CP028116:c4666495-4666184 [Escherichia coli O26 str. RM8426]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T100551 NZ_CP028116:c4666495-4666184 [Escherichia coli O26 str. RM8426]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT100551 WP_000356397.1 NZ_CP028116:c4666183-4665893 [Escherichia coli O26 str. RM8426]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT100551 NZ_CP028116:c4666183-4665893 [Escherichia coli O26 str. RM8426]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7