Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2810483..2810985 Replicon chromosome
Accession NZ_CP028116
Organism Escherichia coli O26 str. RM8426

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag I3M_RS16370 Protein ID WP_000767829.1
Coordinates 2810483..2810737 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I3M_RS16375 Protein ID WP_001259255.1
Coordinates 2810734..2810985 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3M_RS16340 2805498..2805725 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
I3M_RS16345 2805739..2806797 - 1059 WP_000492324.1 transport protein YeeE -
I3M_RS16350 2806976..2808334 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
I3M_RS16355 2808324..2808386 - 63 WP_010723108.1 membrane protein YoeI -
I3M_RS16360 2808601..2809530 - 930 WP_000803351.1 LysR family transcriptional regulator -
I3M_RS16365 2809576..2810400 - 825 WP_000754737.1 SDR family oxidoreductase -
I3M_RS16370 2810483..2810737 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I3M_RS16375 2810734..2810985 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I3M_RS16380 2811268..2811315 + 48 WP_001375657.1 his operon leader peptide -
I3M_RS16390 2811461..2812360 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
I3M_RS16395 2812366..2813670 + 1305 WP_000009589.1 histidinol dehydrogenase -
I3M_RS16400 2813667..2814737 + 1071 WP_000108914.1 histidinol-phosphate transaminase -
I3M_RS16405 2814737..2815804 + 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T100545 WP_000767829.1 NZ_CP028116:c2810737-2810483 [Escherichia coli O26 str. RM8426]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T100545 NZ_CP028116:c2810737-2810483 [Escherichia coli O26 str. RM8426]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT100545 WP_001259255.1 NZ_CP028116:c2810985-2810734 [Escherichia coli O26 str. RM8426]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT100545 NZ_CP028116:c2810985-2810734 [Escherichia coli O26 str. RM8426]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References