Detailed information of TA system TA100528

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1875761..1876132
• Replicon: chromosome
• Accession: NZ_CP028116
• Organism: Escherichia coli O26 str. RM8426

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: I3M_RS11090
• Protein ID: WP_001443846.1
• Coordinates: 1875983..1876132 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1875761..1875939 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3M_RS11065	1871716..1873089	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
I3M_RS11070	1873218..1874153	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
I3M_RS11075	1874205..1875440	-	1236	WP_000040851.1	site-specific integrase	-
I3M_RS11080	1875442..1875657	-	216	WP_000079604.1	excisionase XisR	-
I3M_RS11085	1875757..1875945	-	189	WP_001302840.1	DUF1187 family protein	-
-	1875761..1875939	+	179	NuclAT_0	-	Antitoxin
-	1875761..1875939	+	179	NuclAT_0	-	Antitoxin
-	1875761..1875939	+	179	NuclAT_0	-	Antitoxin
-	1875761..1875939	+	179	NuclAT_0	-	Antitoxin
I3M_RS11090	1875983..1876132	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
I3M_RS11095	1876188..1876997	-	810	WP_000166313.1	recombination protein RecT	-
I3M_RS11100	1876990..1879590	-	2601	WP_000105151.1	exodeoxyribonuclease VIII	-
I3M_RS11105	1879692..1879967	-	276	WP_000632297.1	protein RacC	-
I3M_RS11110	1880042..1880212	-	171	WP_001352098.1	conserved protein, Rac prophage	-
I3M_RS11115	1880212..1880433	-	222	WP_000560223.1	killing protein KilR	-
I3M_RS11120	1880854..1881006	-	153	WP_001427316.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1874205..1920196	45991

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T100528 WP_001443846.1 NZ_CP028116:c1876132-1875983 [Escherichia coli O26 str. RM8426]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T100528 NZ_CP028116:c1876132-1875983 [Escherichia coli O26 str. RM8426]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT100528 NZ_CP028116:1875761-1875939 [Escherichia coli O26 str. RM8426]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin