Detailed information of TA system TA100512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4889799..4890401
• Replicon: chromosome
• Accession: NZ_CP028112
• Organism: Escherichia coli O103 str. RM8385

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I3Q_RS26465
• Protein ID: WP_000897305.1
• Coordinates: 4889799..4890110 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I3Q_RS26470
• Protein ID: WP_000356397.1
• Coordinates: 4890111..4890401 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3Q_RS26435	4884829..4885614	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
I3Q_RS26440	4885713..4886312	+	600	WP_001315111.1	glucose-1-phosphatase	-
I3Q_RS26445	4886306..4887178	+	873	WP_000920762.1	virulence factor BrkB family protein	-
I3Q_RS26450	4887175..4887612	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I3Q_RS26455	4887657..4888598	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I3Q_RS26460	4888662..4889570	-	909	WP_001162704.1	alpha/beta hydrolase	-
I3Q_RS26465	4889799..4890110	+	312	WP_000897305.1	hypothetical protein	Toxin
I3Q_RS26470	4890111..4890401	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I3Q_RS26480	4891006..4891224	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
I3Q_RS26485	4891443..4891685	+	243	WP_001086390.1	hypothetical protein	-
I3Q_RS26490	4891914..4892894	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
I3Q_RS26500	4893294..4894223	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
I3Q_RS26505	4894220..4894855	-	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4891914..4892894	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T100512 WP_000897305.1 NZ_CP028112:4889799-4890110 [Escherichia coli O103 str. RM8385]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T100512 NZ_CP028112:4889799-4890110 [Escherichia coli O103 str. RM8385]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT100512 WP_000356397.1 NZ_CP028112:4890111-4890401 [Escherichia coli O103 str. RM8385]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT100512 NZ_CP028112:4890111-4890401 [Escherichia coli O103 str. RM8385]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7