Detailed information of TA system TA100508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3576355..3577082
• Replicon: chromosome
• Accession: NZ_CP028112
• Organism: Escherichia coli O103 str. RM8385

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: I3Q_RS19660
• Protein ID: WP_000547559.1
• Coordinates: 3576771..3577082 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I3Q_RS19655
• Protein ID: WP_000126294.1
• Coordinates: 3576355..3576774 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3Q_RS19635	3571405..3571932	-	528	WP_001078781.1	electron transport protein HydN	-
I3Q_RS19640	3572081..3573091	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
I3Q_RS19645	3573351..3574808	+	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
I3Q_RS19650	3574817..3576241	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
I3Q_RS19655	3576355..3576774	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
I3Q_RS19660	3576771..3577082	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
I3Q_RS19665	3577256..3577999	-	744	WP_001341829.1	hypothetical protein	-
I3Q_RS19670	3578042..3578512	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
I3Q_RS19675	3578505..3578915	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
I3Q_RS19680	3578912..3579679	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
I3Q_RS19685	3579679..3580221	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
I3Q_RS19690	3580231..3581940	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T100508 WP_000547559.1 NZ_CP028112:c3577082-3576771 [Escherichia coli O103 str. RM8385]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T100508 NZ_CP028112:c3577082-3576771 [Escherichia coli O103 str. RM8385]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT100508 WP_000126294.1 NZ_CP028112:c3576774-3576355 [Escherichia coli O103 str. RM8385]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT100508 NZ_CP028112:c3576774-3576355 [Escherichia coli O103 str. RM8385]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163