Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1069552..1070054 Replicon chromosome
Accession NZ_CP028112
Organism Escherichia coli O103 str. RM8385

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag I3Q_RS05490 Protein ID WP_000767829.1
Coordinates 1069800..1070054 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I3Q_RS05485 Protein ID WP_001259255.1
Coordinates 1069552..1069803 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3Q_RS05455 1064730..1065797 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
I3Q_RS05460 1065797..1066867 - 1071 WP_000108980.1 histidinol-phosphate transaminase -
I3Q_RS05465 1066864..1068168 - 1305 WP_001298841.1 histidinol dehydrogenase -
I3Q_RS05470 1068174..1069073 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
I3Q_RS05480 1069219..1069269 - 51 WP_001368802.1 his operon leader peptide -
I3Q_RS05485 1069552..1069803 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I3Q_RS05490 1069800..1070054 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I3Q_RS05495 1070137..1070961 + 825 WP_000754737.1 SDR family oxidoreductase -
I3Q_RS05500 1071007..1071936 + 930 WP_000803351.1 LysR family transcriptional regulator -
I3Q_RS05505 1072151..1072213 + 63 WP_010723108.1 membrane protein YoeI -
I3Q_RS05510 1072203..1073561 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
I3Q_RS05515 1073740..1074798 + 1059 WP_000492346.1 transport protein YeeE -
I3Q_RS05520 1074812..1075039 + 228 WP_000985280.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T100490 WP_000767829.1 NZ_CP028112:1069800-1070054 [Escherichia coli O103 str. RM8385]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T100490 NZ_CP028112:1069800-1070054 [Escherichia coli O103 str. RM8385]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT100490 WP_001259255.1 NZ_CP028112:1069552-1069803 [Escherichia coli O103 str. RM8385]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT100490 NZ_CP028112:1069552-1069803 [Escherichia coli O103 str. RM8385]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References