Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4351930..4352432 Replicon chromosome
Accession NZ_CP028110
Organism Escherichia coli O121 str. RM8352

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag I3S_RS22860 Protein ID WP_000767829.1
Coordinates 4352178..4352432 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I3S_RS22855 Protein ID WP_001259255.1
Coordinates 4351930..4352181 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3S_RS22825 4347108..4348175 - 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
I3S_RS22830 4348175..4349245 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
I3S_RS22835 4349242..4350546 - 1305 WP_000009587.1 histidinol dehydrogenase -
I3S_RS22840 4350552..4351451 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
I3S_RS22850 4351597..4351647 - 51 WP_001364200.1 his operon leader peptide -
I3S_RS22855 4351930..4352181 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I3S_RS22860 4352178..4352432 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I3S_RS22865 4352515..4353339 + 825 WP_000754721.1 SDR family oxidoreductase -
I3S_RS22870 4353385..4354314 + 930 WP_000803351.1 LysR family transcriptional regulator -
I3S_RS22875 4354529..4354591 + 63 WP_010723108.1 membrane protein YoeI -
I3S_RS22880 4354581..4355939 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
I3S_RS22885 4356118..4357176 + 1059 WP_000492305.1 transport protein YeeE -
I3S_RS22890 4357190..4357417 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T100476 WP_000767829.1 NZ_CP028110:4352178-4352432 [Escherichia coli O121 str. RM8352]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T100476 NZ_CP028110:4352178-4352432 [Escherichia coli O121 str. RM8352]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT100476 WP_001259255.1 NZ_CP028110:4351930-4352181 [Escherichia coli O121 str. RM8352]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT100476 NZ_CP028110:4351930-4352181 [Escherichia coli O121 str. RM8352]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References