Detailed information of TA system TA100474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3526305..3527032
• Replicon: chromosome
• Accession: NZ_CP028110
• Organism: Escherichia coli O121 str. RM8352

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: I3S_RS18655
• Protein ID: WP_000547564.1
• Coordinates: 3526305..3526616 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I3S_RS18660
• Protein ID: WP_000126294.1
• Coordinates: 3526613..3527032 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3S_RS18625	3521447..3523156	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
I3S_RS18630	3523166..3523708	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
I3S_RS18635	3523708..3524475	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
I3S_RS18640	3524472..3524882	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
I3S_RS18645	3524875..3525345	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
I3S_RS18650	3525370..3526131	+	762	WP_001026445.1	hypothetical protein	-
I3S_RS18655	3526305..3526616	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
I3S_RS18660	3526613..3527032	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
I3S_RS18665	3527147..3528571	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
I3S_RS18670	3528580..3529956	-	1377	WP_001107877.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
I3S_RS18675	3530216..3531226	+	1011	WP_001343660.1	DNA-binding transcriptional regulator AscG	-
I3S_RS18680	3531375..3531902	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T100474 WP_000547564.1 NZ_CP028110:3526305-3526616 [Escherichia coli O121 str. RM8352]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T100474 NZ_CP028110:3526305-3526616 [Escherichia coli O121 str. RM8352]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT100474 WP_000126294.1 NZ_CP028110:3526613-3527032 [Escherichia coli O121 str. RM8352]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT100474 NZ_CP028110:3526613-3527032 [Escherichia coli O121 str. RM8352]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAAAGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5