Detailed information of TA system TA100465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2146527..2147129
• Replicon: chromosome
• Accession: NZ_CP028110
• Organism: Escherichia coli O121 str. RM8352

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I3S_RS11425
• Protein ID: WP_000897305.1
• Coordinates: 2146818..2147129 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I3S_RS11420
• Protein ID: WP_000356397.1
• Coordinates: 2146527..2146817 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3S_RS11390	2142472..2143374	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I3S_RS11395	2143371..2144006	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I3S_RS11400	2144003..2144932	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
I3S_RS11405	2145262..2145504	-	243	WP_001086388.1	hypothetical protein	-
I3S_RS11410	2145723..2145941	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
I3S_RS11420	2146527..2146817	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I3S_RS11425	2146818..2147129	-	312	WP_000897305.1	hypothetical protein	Toxin
I3S_RS11430	2147358..2148265	+	908	Protein_2082	alpha/beta hydrolase	-
I3S_RS11440	2148433..2149347	-	915	WP_072097029.1	transposase	-
I3S_RS11445	2149360..2150298	-	939	Protein_2084	hypothetical protein	-
I3S_RS11450	2150662..2151603	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I3S_RS11455	2151648..2152085	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T100465 WP_000897305.1 NZ_CP028110:c2147129-2146818 [Escherichia coli O121 str. RM8352]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T100465 NZ_CP028110:c2147129-2146818 [Escherichia coli O121 str. RM8352]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT100465 WP_000356397.1 NZ_CP028110:c2146817-2146527 [Escherichia coli O121 str. RM8352]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT100465 NZ_CP028110:c2146817-2146527 [Escherichia coli O121 str. RM8352]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7