Detailed information of TA system TA100459

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1653504..1653875
• Replicon: chromosome
• Accession: NZ_CP028110
• Organism: Escherichia coli O121 str. RM8352

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: I3S_RS08910
• Protein ID: WP_001317028.1
• Coordinates: 1653681..1653875 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1653504..1653682 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3S_RS08885	1649648..1650583	-	936	WP_001157421.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
I3S_RS08890	1650635..1651813	-	1179	WP_001351710.1	site-specific integrase	-
I3S_RS08900	1653185..1653400	-	216	WP_000079604.1	excisionase XisR	-
I3S_RS08905	1653479..1653688	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1653504..1653682	+	179	NuclAT_0	-	Antitoxin
-	1653504..1653682	+	179	NuclAT_0	-	Antitoxin
-	1653504..1653682	+	179	NuclAT_0	-	Antitoxin
-	1653504..1653682	+	179	NuclAT_0	-	Antitoxin
I3S_RS08910	1653681..1653875	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
I3S_RS08915	1653932..1654741	-	810	WP_000595429.1	recombination protein RecT	-
I3S_RS08920	1654734..1657403	-	2670	WP_000102191.1	exodeoxyribonuclease VIII	-
I3S_RS08925	1657484..1657660	-	177	WP_000245534.1	hypothetical protein	-
I3S_RS08930	1657654..1657875	-	222	WP_000560223.1	killing protein KilR	-
I3S_RS08935	1658300..1658452	-	153	WP_001169149.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	1650635..1701018	50383

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T100459 WP_001317028.1 NZ_CP028110:c1653875-1653681 [Escherichia coli O121 str. RM8352]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T100459 NZ_CP028110:c1653875-1653681 [Escherichia coli O121 str. RM8352]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT100459 NZ_CP028110:1653504-1653682 [Escherichia coli O121 str. RM8352]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin