Detailed information of TA system TA100412

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 83466..83892
• Replicon: plasmid pCRKP18622-KPC
• Accession: NZ_CP027866
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain CRKP18622

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: C7K64_RS28210
• Protein ID: WP_001372321.1
• Coordinates: 83466..83591 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	83668..83892 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7K64_RS28175 (78489)	78489..78716	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
C7K64_RS28180 (78853)	78853..79524	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
C7K64_RS28185 (79718)	79718..80101	-	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
C7K64_RS28190 (80436)	80436..81026	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
C7K64_RS28195 (81323)	81323..82144	-	822	WP_001234445.1	DUF932 domain-containing protein	-
C7K64_RS28200 (82255)	82255..82551	-	297	WP_001272251.1	hypothetical protein	-
C7K64_RS28205 (82851)	82851..83147	+	297	Protein_102	hypothetical protein	-
C7K64_RS28210 (83466)	83466..83591	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
C7K64_RS28215 (83533)	83533..83682	-	150	Protein_104	plasmid maintenance protein Mok	-
- (83668)	83668..83892	-	225	NuclAT_0	-	Antitoxin
- (83668)	83668..83892	-	225	NuclAT_0	-	Antitoxin
- (83668)	83668..83892	-	225	NuclAT_0	-	Antitoxin
- (83668)	83668..83892	-	225	NuclAT_0	-	Antitoxin
C7K64_RS28220 (83904)	83904..84623	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
C7K64_RS28225 (84620)	84620..85054	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
C7K64_RS28230 (85123)	85123..87146	-	2024	Protein_107	ParB/RepB/Spo0J family partition protein	-
C7K64_RS28235 (87207)	87207..87440	-	234	WP_000006003.1	DUF905 family protein	-
C7K64_RS28240 (87498)	87498..88025	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
C7K64_RS28245 (88327)	88327..88776	+	450	Protein_110	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	cmlA1 / qacE / blaCTX-M-14 / blaTEM-1B / blaKPC-2 / sitABCD	iutA / iucD / iucC / iucB / iucA	1..134794	134794

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T100412 WP_001372321.1 NZ_CP027866:c83591-83466 [Klebsiella pneumoniae subsp. pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T100412 NZ_CP027866:c83591-83466 [Klebsiella pneumoniae subsp. pneumoniae]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT100412 NZ_CP027866:c83892-83668 [Klebsiella pneumoniae subsp. pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin