Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 234133..234860 | Replicon | plasmid pVH1-2-VIR |
| Accession | NZ_CP027803 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain VH1-2 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | C7K65_RS29240 | Protein ID | WP_011251285.1 |
| Coordinates | 234133..234444 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | C7K65_RS29245 | Protein ID | WP_011251286.1 |
| Coordinates | 234441..234860 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C7K65_RS29200 (C7K65_30250) | 229194..229709 | - | 516 | WP_041937820.1 | hypothetical protein | - |
| C7K65_RS29205 (C7K65_30255) | 229817..230392 | - | 576 | WP_074424927.1 | hypothetical protein | - |
| C7K65_RS29210 (C7K65_30260) | 230509..231465 | - | 957 | WP_011251280.1 | DsbA family protein | - |
| C7K65_RS29215 (C7K65_30265) | 231525..231866 | - | 342 | WP_011251281.1 | hypothetical protein | - |
| C7K65_RS29220 (C7K65_30270) | 231880..232191 | - | 312 | WP_011251282.1 | hypothetical protein | - |
| C7K65_RS29225 (C7K65_30275) | 232208..232657 | - | 450 | WP_011251283.1 | thioredoxin fold domain-containing protein | - |
| C7K65_RS29235 (C7K65_30285) | 233491..233928 | + | 438 | Protein_283 | DDE-type integrase/transposase/recombinase | - |
| C7K65_RS29240 (C7K65_30290) | 234133..234444 | + | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| C7K65_RS29245 (C7K65_30295) | 234441..234860 | + | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| C7K65_RS29250 (C7K65_30305) | 235007..235975 | + | 969 | WP_074428168.1 | IS5 family transposase | - |
| C7K65_RS29255 (C7K65_30310) | 236047..236412 | - | 366 | WP_048333448.1 | hypothetical protein | - |
| C7K65_RS29260 (C7K65_30315) | 236426..237214 | - | 789 | WP_040217257.1 | hypothetical protein | - |
| C7K65_RS29265 (C7K65_30320) | 237235..237855 | - | 621 | WP_223175004.1 | conjugative transfer protein MobI(A/C) | - |
| C7K65_RS29270 | 238274..238909 | + | 636 | WP_223171879.1 | hypothetical protein | - |
| C7K65_RS29275 (C7K65_30335) | 239222..239692 | + | 471 | WP_048333570.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | mph(A) / sul1 / qacE / qnrB6 / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1C | rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB | 1..346781 | 346781 | |
| - | inside | IScluster/Tn | - | - | 232659..244981 | 12322 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T100360 WP_011251285.1 NZ_CP027803:234133-234444 [Klebsiella pneumoniae subsp. pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T100360 NZ_CP027803:234133-234444 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT100360 WP_011251286.1 NZ_CP027803:234441-234860 [Klebsiella pneumoniae subsp. pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT100360 NZ_CP027803:234441-234860 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|