Detailed information of TA system TA100352

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 83642..84068
• Replicon: plasmid pVH1-2-KPC
• Accession: NZ_CP027802
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain VH1-2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: C7K65_RS27535
• Protein ID: WP_001372321.1
• Coordinates: 83642..83767 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	83844..84068 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7K65_RS27500 (78665)	78665..78892	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
C7K65_RS27505 (79029)	79029..79700	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
C7K65_RS27510 (79894)	79894..80277	-	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
C7K65_RS27515 (80612)	80612..81202	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
C7K65_RS27520 (81499)	81499..82320	-	822	WP_001234445.1	DUF932 domain-containing protein	-
C7K65_RS27525 (82431)	82431..82727	-	297	WP_001272251.1	hypothetical protein	-
C7K65_RS27530 (83027)	83027..83323	+	297	Protein_100	hypothetical protein	-
C7K65_RS27535 (83642)	83642..83767	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
C7K65_RS27540 (83709)	83709..83858	-	150	Protein_102	plasmid maintenance protein Mok	-
- (83844)	83844..84068	-	225	NuclAT_0	-	Antitoxin
- (83844)	83844..84068	-	225	NuclAT_0	-	Antitoxin
- (83844)	83844..84068	-	225	NuclAT_0	-	Antitoxin
- (83844)	83844..84068	-	225	NuclAT_0	-	Antitoxin
C7K65_RS27545 (84080)	84080..84799	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
C7K65_RS27550 (84796)	84796..85230	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
C7K65_RS27555 (85299)	85299..87322	-	2024	Protein_105	ParB/RepB/Spo0J family partition protein	-
C7K65_RS27560 (87383)	87383..87616	-	234	WP_000006003.1	DUF905 family protein	-
C7K65_RS27565 (87674)	87674..88201	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
C7K65_RS27570 (88503)	88503..88952	+	450	Protein_108	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	cmlA1 / blaTEM-1B / blaKPC-2 / sitABCD	iutA / iucD / iucC / iucB / iucA	1..128932	128932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T100352 WP_001372321.1 NZ_CP027802:c83767-83642 [Klebsiella pneumoniae subsp. pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T100352 NZ_CP027802:c83767-83642 [Klebsiella pneumoniae subsp. pneumoniae]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT100352 NZ_CP027802:c84068-83844 [Klebsiella pneumoniae subsp. pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin