Detailed information of TA system TA100305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-DinJ
• Location: 2515643..2516162
• Replicon: chromosome
• Accession: NZ_CP027786
• Organism: Tetragenococcus koreensis strain KCTC 3924

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: C7K43_RS12010
• Protein ID: WP_124007050.1
• Coordinates: 2515878..2516162 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: C7K43_RS12005
• Protein ID: WP_124007049.1
• Coordinates: 2515643..2515888 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7K43_RS11975	2511697..2511927	+	231	WP_124007043.1	antitoxin	-
C7K43_RS11980	2511924..2512190	+	267	WP_124007044.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C7K43_RS11985	2512378..2512560	-	183	WP_124007045.1	putative holin-like toxin	-
C7K43_RS11990	2512748..2513395	-	648	WP_124007046.1	pyroglutamyl-peptidase I	-
C7K43_RS11995	2513722..2514642	-	921	WP_124007047.1	DUF979 domain-containing protein	-
C7K43_RS12000	2514626..2515333	-	708	WP_124007048.1	DUF969 domain-containing protein	-
C7K43_RS12005	2515643..2515888	+	246	WP_124007049.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
C7K43_RS12010	2515878..2516162	+	285	WP_124007050.1	type II toxin-antitoxin system YafQ family toxin	Toxin
C7K43_RS12015	2516411..2518252	+	1842	WP_124007051.1	beta-glucoside-specific PTS transporter subunit IIABC	-
C7K43_RS12020	2518274..2519746	+	1473	Protein_2330	family 1 glycosylhydrolase	-
C7K43_RS12025	2519765..2520604	+	840	WP_124007052.1	PRD domain-containing protein	-
C7K43_RS12030	2520816..2521064	+	249	WP_124007053.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (11-93)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-57)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11689.58 Da
• Isoelectric Point: 10.2072

>T100305 WP_124007050.1 NZ_CP027786:2515878-2516162 [Tetragenococcus koreensis]
MRNKPKYKPAFERKFKKHYRNMLKGGRYKKEDFEKVYWKLLCNEPLEPRYNDHPLVNRKPERDLHIKPDWLLIYKYDGEF
VRFIDTGTHADLFK

Nucleotide

• Length: 285 bp

>T100305 NZ_CP027786:2515878-2516162 [Tetragenococcus koreensis]
ATGAGGAATAAGCCTAAATATAAGCCTGCTTTTGAGCGGAAGTTTAAAAAGCATTATAGAAATATGCTTAAGGGCGGACG
TTATAAAAAAGAAGACTTTGAAAAAGTTTATTGGAAACTTTTGTGTAACGAACCATTAGAACCTCGATACAACGATCATC
CTTTAGTAAATCGAAAACCTGAACGTGACTTGCATATTAAACCGGATTGGCTATTGATTTATAAATATGACGGCGAGTTT
GTCCGATTTATTGACACGGGCACTCATGCGGATCTCTTTAAATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9484.50 Da
• Isoelectric Point: 4.0718

>AT100305 WP_124007049.1 NZ_CP027786:2515643-2515888 [Tetragenococcus koreensis]
MTNQVHFRIDEEDKEKFRIVMQHVGLEPNEAYRIFVKRAIEVGGIPFEVADPSSQLEEAIKSQDYIEFENGEEGLDWLNE
E

Nucleotide

• Length: 246 bp

>AT100305 NZ_CP027786:2515643-2515888 [Tetragenococcus koreensis]
ATGACTAATCAAGTGCATTTTAGAATAGATGAAGAAGATAAAGAAAAGTTTAGAATAGTGATGCAACACGTAGGGTTAGA
GCCTAATGAGGCTTATCGTATCTTTGTTAAAAGAGCAATTGAAGTTGGAGGAATTCCGTTTGAAGTTGCAGATCCATCTT
CTCAATTAGAGGAAGCAATTAAAAGTCAGGATTATATTGAATTTGAAAACGGTGAGGAAGGCTTGGATTGGTTAAATGAG
GAATAA