Detailed information of TA system TA100288

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 52343..52612
• Replicon: plasmid unnamed
• Accession: NZ_CP027767
• Organism: Escherichia coli strain 2013C-3342

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C7M85_RS29915
• Protein ID: WP_001312861.1
• Coordinates: 52454..52612 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	52343..52408 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M85_RS29870	47415..47663	+	249	WP_071606928.1	hypothetical protein	-
C7M85_RS29890	48136..48663	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
C7M85_RS29895	48719..48952	+	234	WP_000006004.1	DUF905 domain-containing protein	-
C7M85_RS29900	49011..50969	+	1959	WP_001145473.1	ParB/RepB/Spo0J family partition protein	-
C7M85_RS29905	51024..51458	+	435	WP_044804925.1	conjugation system SOS inhibitor PsiB	-
C7M85_RS29910	51455..52174	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
C7M85_RS30420	52186..52374	-	189	WP_001299721.1	hypothetical protein	-
-	52186..52410	+	225	NuclAT_0	-	-
-	52186..52410	+	225	NuclAT_0	-	-
-	52186..52410	+	225	NuclAT_0	-	-
-	52186..52410	+	225	NuclAT_0	-	-
-	52343..52408	+	66	-	-	Antitoxin
C7M85_RS29915	52454..52612	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
C7M85_RS30655	53303..53509	+	207	WP_000547971.1	hypothetical protein	-
C7M85_RS29935	53534..53821	+	288	WP_000107535.1	hypothetical protein	-
C7M85_RS30660	53941..54024	+	84	Protein_60	DUF945 domain-containing protein	-
C7M85_RS29945	54209..55396	-	1188	WP_106884016.1	IS91 family transposase	-
C7M85_RS29955	55685..56665	-	981	WP_000019402.1	IS5-like element IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyD / espP	1..66545	66545

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T100288 WP_001312861.1 NZ_CP027767:52454-52612 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T100288 NZ_CP027767:52454-52612 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT100288 NZ_CP027767:52343-52408 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin