Detailed information of TA system TA100282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4235111..4235713
• Replicon: chromosome
• Accession: NZ_CP027766
• Organism: Escherichia coli strain 2013C-3342

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C7M85_RS22980
• Protein ID: WP_000897305.1
• Coordinates: 4235111..4235422 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C7M85_RS22985
• Protein ID: WP_100039178.1
• Coordinates: 4235423..4235713 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M85_RS22950	4230141..4230926	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
C7M85_RS22955	4231025..4231624	+	600	WP_001315111.1	glucose-1-phosphatase	-
C7M85_RS22960	4231618..4232490	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C7M85_RS22965	4232487..4232924	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C7M85_RS22970	4232969..4233910	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C7M85_RS22975	4233974..4234882	-	909	WP_001162704.1	alpha/beta hydrolase	-
C7M85_RS22980	4235111..4235422	+	312	WP_000897305.1	hypothetical protein	Toxin
C7M85_RS22985	4235423..4235713	+	291	WP_100039178.1	helix-turn-helix domain-containing protein	Antitoxin
C7M85_RS22995	4236318..4236536	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C7M85_RS23000	4236755..4236997	+	243	WP_001086388.1	hypothetical protein	-
C7M85_RS23010	4237328..4238257	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C7M85_RS23015	4238254..4238889	-	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
C7M85_RS23020	4238886..4239788	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T100282 WP_000897305.1 NZ_CP027766:4235111-4235422 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T100282 NZ_CP027766:4235111-4235422 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCTCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10793.38 Da
• Isoelectric Point: 10.1864

>AT100282 WP_100039178.1 NZ_CP027766:4235423-4235713 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRCASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT100282 NZ_CP027766:4235423-4235713 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCTGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7