Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2042710..2043212 Replicon chromosome
Accession NZ_CP027766
Organism Escherichia coli strain 2013C-3342

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C7M85_RS11555 Protein ID WP_000767829.1
Coordinates 2042710..2042964 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C7M85_RS11560 Protein ID WP_001259255.1
Coordinates 2042961..2043212 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7M85_RS11525 2037725..2037952 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
C7M85_RS11530 2037966..2039024 - 1059 WP_044805324.1 transport protein YeeE -
C7M85_RS11535 2039203..2040561 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C7M85_RS11540 2040551..2040613 - 63 WP_010723108.1 membrane protein YoeI -
C7M85_RS11545 2040828..2041757 - 930 WP_000803337.1 LysR family transcriptional regulator -
C7M85_RS11550 2041803..2042627 - 825 WP_000754758.1 SDR family oxidoreductase -
C7M85_RS11555 2042710..2042964 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C7M85_RS11560 2042961..2043212 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C7M85_RS11565 2043495..2043545 + 51 WP_001368802.1 his operon leader peptide -
C7M85_RS11575 2043691..2044590 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C7M85_RS11580 2044596..2045900 + 1305 WP_106884165.1 histidinol dehydrogenase -
C7M85_RS11585 2045897..2046967 + 1071 WP_106884166.1 histidinol-phosphate transaminase -
C7M85_RS11590 2046967..2048034 + 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T100275 WP_000767829.1 NZ_CP027766:c2042964-2042710 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T100275 NZ_CP027766:c2042964-2042710 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT100275 WP_001259255.1 NZ_CP027766:c2043212-2042961 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT100275 NZ_CP027766:c2043212-2042961 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References