Detailed information of TA system TA100251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1353552..1354159
• Replicon: chromosome
• Accession: NZ_CP027761
• Organism: Pseudomonas sp. R11-23-07

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C4J84_RS06010
• Protein ID: WP_124369842.1
• Coordinates: 1353845..1354159 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C4J84_RS06005
• Protein ID: WP_124369841.1
• Coordinates: 1353552..1353845 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J84_RS05970	1348654..1349349	-	696	WP_124369836.1	tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO	-
C4J84_RS05975	1349353..1349814	-	462	WP_124369837.1	DUF1456 family protein	-
C4J84_RS05980	1349885..1350595	-	711	WP_124369838.1	rRNA pseudouridine synthase	-
C4J84_RS05985	1350705..1351175	-	471	WP_124369839.1	GNAT family N-acetyltransferase	-
C4J84_RS05990	1351276..1352604	-	1329	WP_177412771.1	30S ribosomal protein S12 methylthiotransferase RimO	-
C4J84_RS05995	1352893..1353183	+	291	WP_124369840.1	hypothetical protein	-
C4J84_RS06000	1353156..1353542	+	387	Protein_1154	hypothetical protein	-
C4J84_RS06005	1353552..1353845	-	294	WP_124369841.1	helix-turn-helix domain-containing protein	Antitoxin
C4J84_RS06010	1353845..1354159	-	315	WP_124369842.1	hypothetical protein	Toxin
C4J84_RS06015	1354930..1356831	+	1902	WP_124361561.1	potassium transporter Kup	-
C4J84_RS06020	1356998..1358104	+	1107	WP_124366074.1	NADH:flavin oxidoreductase/NADH oxidase	-
C4J84_RS06025	1358326..1358511	+	186	WP_124369843.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-72)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11787.48 Da
• Isoelectric Point: 8.3275

>T100251 WP_124369842.1 NZ_CP027761:c1354159-1353845 [Pseudomonas sp. R11-23-07]
VIFIESPIFTEDLGELLSDESYREFQEYLAENPTAGDVIQSTNGLRKVRWSANGKGKSGGVRVIYFYLSAAYQIRLILIY
RKGIKDDLSPAEKKVLAELNKGWK

Nucleotide

• Length: 315 bp

>T100251 NZ_CP027761:c1354159-1353845 [Pseudomonas sp. R11-23-07]
GTGATATTCATTGAGTCCCCCATCTTCACGGAAGATCTTGGCGAGCTTCTGAGCGATGAAAGCTACCGCGAATTTCAGGA
GTACCTAGCCGAAAACCCAACGGCTGGTGATGTGATCCAAAGCACCAACGGACTGAGGAAGGTCCGCTGGAGCGCGAACG
GGAAAGGTAAGAGCGGTGGGGTTCGGGTGATCTATTTCTACCTATCGGCGGCTTACCAGATCAGGCTGATCCTGATCTAC
CGTAAGGGGATAAAAGACGATCTATCCCCAGCGGAAAAGAAAGTACTGGCAGAGTTAAACAAAGGGTGGAAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11001.59 Da
• Isoelectric Point: 10.1711

>AT100251 WP_124369841.1 NZ_CP027761:c1353845-1353552 [Pseudomonas sp. R11-23-07]
MEKNLFNRLVESMTQMDEIDRGERPPSREFHVDAVKVKMIRKATGLSQARFAKAIDVAVGTLQNWEQGRREPEGPARALL
RAIHNDPGHVLAALEQR

Nucleotide

• Length: 294 bp

>AT100251 NZ_CP027761:c1353845-1353552 [Pseudomonas sp. R11-23-07]
ATGGAAAAGAATCTGTTCAACCGCCTGGTCGAAAGCATGACCCAGATGGACGAGATTGACCGTGGCGAACGCCCGCCGTC
CCGTGAGTTCCATGTGGATGCGGTGAAGGTCAAAATGATCCGCAAGGCCACAGGACTTTCCCAGGCACGTTTTGCCAAAG
CGATTGATGTCGCGGTGGGAACGCTCCAAAACTGGGAGCAAGGTCGGCGGGAGCCAGAAGGCCCCGCACGGGCACTCCTG
CGCGCCATACACAATGATCCTGGGCACGTATTAGCGGCTCTTGAGCAACGCTAG