Detailed information of TA system TA100213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1793334..1793938
• Replicon: chromosome
• Accession: NZ_CP027756
• Organism: Pseudomonas synxantha strain R6-28-08

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C4K01_RS08320
• Protein ID: WP_124356083.1
• Coordinates: 1793624..1793938 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C4K01_RS08315
• Protein ID: WP_057022035.1
• Coordinates: 1793334..1793621 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K01_RS08275	1788595..1789353	+	759	WP_056847351.1	MBL fold metallo-hydrolase	-
C4K01_RS08280	1789381..1790094	+	714	WP_057022031.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C4K01_RS08290	1790363..1790656	-	294	WP_057022032.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C4K01_RS08295	1790646..1790885	-	240	WP_014717442.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
C4K01_RS08305	1791539..1791793	+	255	WP_057022033.1	hypothetical protein	-
C4K01_RS08310	1791868..1793094	-	1227	WP_057022034.1	MFS transporter	-
C4K01_RS08315	1793334..1793621	-	288	WP_057022035.1	helix-turn-helix domain-containing protein	Antitoxin
C4K01_RS08320	1793624..1793938	-	315	WP_124356083.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K01_RS08325	1794044..1794985	-	942	WP_057022037.1	LysR family transcriptional regulator	-
C4K01_RS08330	1795143..1796459	+	1317	WP_057022038.1	MHS family MFS transporter	-
C4K01_RS08335	1796471..1797703	+	1233	WP_057022039.1	Zn-dependent hydrolase	-
C4K01_RS08340	1797838..1798875	+	1038	WP_057022040.1	histone deacetylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	mucA / mucD	1780183..1803154	22971

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (16-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11686.46 Da
• Isoelectric Point: 9.8054

>T100213 WP_124356083.1 NZ_CP027756:c1793938-1793624 [Pseudomonas synxantha]
MIFIETSVFTRRVKELIDEDAYTALQNVLVVNPSAGDVIEGTGGVRKIRVATKGHGKRGGARVIYYHFVSASQIVLLMIY
PKNEQQDLTADERKSLKAAIEHWR

Nucleotide

• Length: 315 bp

>T100213 NZ_CP027756:c1793938-1793624 [Pseudomonas synxantha]
ATGATTTTCATAGAGACGTCAGTCTTCACCCGTCGCGTTAAAGAATTGATCGACGAGGATGCCTACACGGCTCTTCAGAA
CGTGTTGGTAGTCAACCCTTCTGCTGGCGATGTAATCGAGGGTACGGGTGGAGTTCGCAAGATACGAGTAGCAACCAAGG
GCCATGGCAAACGAGGCGGGGCGCGGGTGATCTATTACCACTTCGTCTCGGCATCACAGATTGTTCTACTGATGATCTAT
CCCAAAAATGAACAGCAGGACTTGACTGCCGACGAGCGCAAGTCACTGAAGGCTGCGATTGAGCACTGGAGATAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10827.42 Da
• Isoelectric Point: 8.8454

>AT100213 WP_057022035.1 NZ_CP027756:c1793621-1793334 [Pseudomonas synxantha]
MSRFFNELMESVQEMNEILRGEREPSREFHVDSMRVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRREPTGPAKALLK
AIHNDPVHVLNALAS

Nucleotide

• Length: 288 bp

>AT100213 NZ_CP027756:c1793621-1793334 [Pseudomonas synxantha]
ATGAGCAGATTTTTTAATGAACTGATGGAAAGCGTTCAAGAGATGAACGAGATCCTGCGCGGAGAACGCGAACCTTCTCG
TGAATTCCATGTTGATTCAATGCGAGTCAAAGAGATCCGCAAGGCGACAGGGCTGACCCAGGCTAAATTCGCAGCGCTGA
TTGATGTGCAACTTGGCACATTACGTAACTGGGAGCAGGGTCGTCGTGAACCTACCGGACCAGCAAAGGCATTGTTGAAA
GCTATCCATAATGACCCAGTCCATGTACTGAATGCGTTGGCTAGCTAA