Detailed information of TA system TA100175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 534967..535661
• Replicon: chromosome
• Accession: NZ_CP027751
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain ChPhzTR18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C4K06_RS02415
• Protein ID: WP_053268076.1
• Coordinates: 534967..535263 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A554PLA7
• Locus tag: C4K06_RS02420
• Protein ID: WP_009046631.1
• Coordinates: 535260..535661 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K06_RS02380	530501..531046	-	546	WP_028683761.1	membrane protein	-
C4K06_RS02385	531043..531297	-	255	WP_007926835.1	acyl carrier protein	-
C4K06_RS02390	531307..531567	-	261	WP_007926834.1	acyl carrier protein	-
C4K06_RS02395	531542..532357	-	816	WP_016704468.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
C4K06_RS02400	532333..533064	-	732	WP_124301067.1	beta-ketoacyl synthase chain length factor	-
C4K06_RS02405	533398..534168	+	771	WP_007926828.1	ParA family protein	-
C4K06_RS02410	534294..534728	-	435	WP_016704469.1	thioredoxin TrxC	-
C4K06_RS02415	534967..535263	+	297	WP_053268076.1	type II toxin-antitoxin system HigB family toxin	Toxin
C4K06_RS02420	535260..535661	+	402	WP_009046631.1	transcriptional regulator	Antitoxin
C4K06_RS02425	535665..537128	-	1464	WP_124301068.1	YdiU family protein	-
C4K06_RS02430	537177..540527	-	3351	WP_009046633.1	mechanosensitive channel MscK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-90)

Antitoxin

• Antitoxin HigA (15-133)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11588.41 Da
• Isoelectric Point: 10.6515

>T100175 WP_053268076.1 NZ_CP027751:534967-535263 [Pseudomonas chlororaphis subsp. aureofaciens]
MHIITAKRIWEAKSRWPQSANALETWYRKVKSLNPADFAAIKAVFPATDKVGAFHVFDIGGNKLRIIAVVRYEVRRLYIR
HVLDHHEYDKGRWKEETR

Nucleotide

• Length: 297 bp

>T100175 NZ_CP027751:534967-535263 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGCATATCATCACCGCCAAACGAATCTGGGAGGCCAAGAGCCGCTGGCCACAATCGGCCAATGCGCTGGAGACCTGGTA
CAGAAAAGTCAAAAGCCTCAATCCGGCCGATTTTGCAGCCATCAAGGCAGTATTTCCGGCCACCGACAAAGTGGGGGCGT
TCCACGTCTTCGATATTGGCGGCAACAAGTTACGAATCATCGCCGTGGTGCGTTATGAGGTGCGACGTCTCTATATCAGG
CACGTGCTGGACCATCACGAATACGACAAGGGCAGGTGGAAGGAGGAGACCAGATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14840.85 Da
• Isoelectric Point: 4.4394

>AT100175 WP_009046631.1 NZ_CP027751:535260-535661 [Pseudomonas chlororaphis subsp. aureofaciens]
MNVLVKQAADHWHFVAPVLRKPNTEADYDALVAALDELLDLMGEDENHPVTSLVDIIGDWIEAYDEEHRPMPIASGVDVL
RSLMREHGLTQSDLPGVGAQSVVSEVLSGKRQLNLRQIRWLAERFGVSAEVFI

Nucleotide

• Length: 402 bp

>AT100175 NZ_CP027751:535260-535661 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGAACGTTCTGGTGAAGCAGGCGGCGGATCATTGGCATTTCGTGGCCCCTGTGTTGCGTAAACCCAATACTGAAGCCGA
TTACGATGCATTGGTCGCGGCGCTGGATGAACTGCTGGATCTCATGGGAGAGGACGAGAACCATCCAGTGACTTCGCTGG
TGGACATCATCGGCGATTGGATCGAAGCCTACGACGAAGAGCATCGACCGATGCCGATAGCCAGTGGTGTTGACGTCTTG
CGCTCCCTGATGCGCGAACACGGCCTGACCCAGAGTGATCTGCCAGGCGTGGGTGCGCAGTCGGTGGTTTCGGAAGTACT
GAGCGGCAAGCGCCAGCTCAACCTGCGACAGATCCGCTGGCTGGCCGAGCGCTTCGGTGTTTCGGCCGAGGTATTTATCT
GA

Structures

Antitoxin

• AlphaFold DB: A0A554PLA7