Detailed information of TA system TA100171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4346681..4347335
• Replicon: chromosome
• Accession: NZ_CP027750
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain ChPhzS24

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C4K07_RS19475
• Protein ID: WP_096376050.1
• Coordinates: 4346681..4347031 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C4K07_RS19480
• Protein ID: WP_009049793.1
• Coordinates: 4347021..4347335 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K07_RS19465	4343431..4344963	+	1533	WP_124301865.1	NADH-quinone oxidoreductase subunit M	-
C4K07_RS19470	4344971..4346434	+	1464	WP_009049791.1	NADH-quinone oxidoreductase subunit NuoN	-
C4K07_RS19475	4346681..4347031	+	351	WP_096376050.1	toxin	Toxin
C4K07_RS19480	4347021..4347335	+	315	WP_009049793.1	transcriptional regulator	Antitoxin
C4K07_RS19485	4347502..4349244	-	1743	WP_023964376.1	ABC transporter substrate-binding protein	-
C4K07_RS19495	4349408..4349680	-	273	WP_009049795.1	DUF2160 domain-containing protein	-
C4K07_RS19500	4349691..4350491	-	801	WP_007929618.1	carbohydrate ABC transporter permease	-
C4K07_RS19505	4350503..4351369	-	867	WP_016701903.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-100)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13803.77 Da
• Isoelectric Point: 9.4187

>T100171 WP_096376050.1 NZ_CP027750:4346681-4347031 [Pseudomonas chlororaphis subsp. aureofaciens]
MDALFIELPPFQRHRQDYLDDELFRSLQLELLKAPEAGDLIEGTGGLRKIRFVDERRHKGKRGGIRVIYYWWSGGAQFWL
FTLYAKNEQNDLTPHQKKLLKQLLNREVEARTHHET

Nucleotide

• Length: 351 bp

>T100171 NZ_CP027750:4346681-4347031 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGGACGCACTTTTTATTGAGCTGCCCCCGTTCCAACGCCATCGACAGGACTACCTGGACGACGAGCTTTTTCGTAGCTT
GCAGCTTGAGTTGCTGAAAGCCCCCGAAGCCGGAGATCTCATCGAGGGAACAGGTGGCTTGCGCAAGATCCGCTTTGTCG
ATGAGCGACGGCACAAGGGCAAACGCGGCGGGATCCGGGTGATTTATTACTGGTGGTCCGGAGGCGCCCAATTCTGGCTC
TTCACCCTTTACGCAAAGAACGAGCAGAACGACCTGACACCCCATCAGAAAAAACTGCTCAAGCAACTGCTCAACAGAGA
AGTCGAGGCGAGAACCCATCATGAAACGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11917.85 Da
• Isoelectric Point: 10.6052

>AT100171 WP_009049793.1 NZ_CP027750:4347021-4347335 [Pseudomonas chlororaphis subsp. aureofaciens]
MKRDIFSELVEGFDALAKERQGKLTLRTYKVKLNALLPITAEEVLAVREQLNLSRSVFAMYLRTNTRTLENWEQGRAKPN
AQATTLIRLVQRFPDTVERLAALA

Nucleotide

• Length: 315 bp

>AT100171 NZ_CP027750:4347021-4347335 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGAAACGTGACATCTTTTCCGAACTGGTCGAAGGTTTCGACGCCCTGGCCAAAGAGCGCCAAGGCAAGCTCACGCTGCG
CACCTACAAGGTCAAGCTGAACGCGCTGCTCCCGATCACCGCTGAAGAAGTGCTGGCCGTACGCGAGCAGTTGAACCTGT
CCAGATCGGTATTTGCCATGTACCTGCGCACCAATACTCGGACCCTGGAGAACTGGGAGCAAGGACGGGCAAAACCCAAC
GCTCAGGCCACTACCCTGATCCGCCTGGTACAGCGTTTTCCCGATACGGTGGAGCGGCTGGCAGCATTGGCCTGA