Detailed information of TA system TA100156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4356029..4356683
• Replicon: chromosome
• Accession: NZ_CP027747
• Organism: Pseudomonas chlororaphis subsp. aureofaciens strain 66

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C4K10_RS19625
• Protein ID: WP_110175823.1
• Coordinates: 4356029..4356379 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C4K10_RS19630
• Protein ID: WP_025804712.1
• Coordinates: 4356369..4356683 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K10_RS19615	4352779..4354311	+	1533	WP_009049790.1	NADH-quinone oxidoreductase subunit M	-
C4K10_RS19620	4354319..4355782	+	1464	WP_009049791.1	NADH-quinone oxidoreductase subunit NuoN	-
C4K10_RS19625	4356029..4356379	+	351	WP_110175823.1	toxin	Toxin
C4K10_RS19630	4356369..4356683	+	315	WP_025804712.1	transcriptional regulator	Antitoxin
C4K10_RS19635	4356850..4358592	-	1743	WP_023964376.1	ABC transporter substrate-binding protein	-
C4K10_RS19645	4358756..4359028	-	273	WP_009049795.1	DUF2160 domain-containing protein	-
C4K10_RS19650	4359039..4359839	-	801	WP_025804715.1	carbohydrate ABC transporter permease	-
C4K10_RS19655	4359851..4360717	-	867	WP_009049796.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-100)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13929.93 Da
• Isoelectric Point: 9.7779

>T100156 WP_110175823.1 NZ_CP027747:4356029-4356379 [Pseudomonas chlororaphis subsp. aureofaciens]
MGALFIELPPFQRHRQDYLDDELFRNLQLELLKAPEAGDLIERTDGLRKIRFVDERRHKGKRGGIRVIYYWWSGGAQFWL
FTLYAKNEQNDLTPHQKKLLKQLLNREVEARTHHET

Nucleotide

• Length: 351 bp

>T100156 NZ_CP027747:4356029-4356379 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGGGCGCACTTTTTATTGAGCTGCCCCCGTTCCAACGCCATCGACAGGACTACCTGGACGACGAGCTTTTTCGTAATTT
GCAGCTTGAGTTGCTGAAAGCCCCCGAAGCCGGAGATCTCATCGAGAGAACCGATGGCTTGCGCAAGATCCGCTTTGTCG
ATGAGCGACGGCACAAGGGCAAACGCGGCGGGATCCGGGTGATTTATTACTGGTGGTCCGGAGGCGCCCAATTCTGGCTC
TTCACCCTTTACGCAAAGAACGAGCAGAACGACCTGACACCCCATCAGAAAAAACTGCTCAAGCAACTGCTCAACAGAGA
AGTCGAGGCGAGAACCCATCATGAAACGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11891.81 Da
• Isoelectric Point: 10.7886

>AT100156 WP_025804712.1 NZ_CP027747:4356369-4356683 [Pseudomonas chlororaphis subsp. aureofaciens]
MKRDIFSELVEGFDALAKERQGKLTLRTHKVKLNALLPITAEEVLAVREQLNLSRSVFAMYLRTNTRTLENWEQGRAKPN
AQATTLIRLVQRFPDTVERLAALA

Nucleotide

• Length: 315 bp

>AT100156 NZ_CP027747:4356369-4356683 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGAAACGTGACATCTTTTCCGAACTGGTCGAAGGTTTCGACGCCCTGGCCAAAGAGCGCCAAGGCAAGCTCACGCTGCG
CACCCACAAGGTCAAGCTGAACGCACTGCTACCGATCACCGCCGAAGAAGTGCTGGCCGTGCGCGAACAGCTGAACCTGT
CCAGATCGGTATTTGCCATGTACCTGCGCACCAATACTCGGACCCTGGAGAACTGGGAGCAAGGACGGGCAAAACCCAAC
GCTCAGGCCACTACCCTGATCCGCCTGGTACAGCGTTTTCCCGATACGGTGGAGCGGCTGGCAGCATTGGCCTGA