Detailed information of TA system TA100111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 2242314..2242969
• Replicon: chromosome
• Accession: NZ_CP027740
• Organism: Pseudomonas chlororaphis subsp. piscium strain ChPhzS140

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7D6EBU8
• Locus tag: C4K29_RS10275
• Protein ID: WP_062823026.1
• Coordinates: 2242314..2242658 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C4K29_RS10280
• Protein ID: WP_124304706.1
• Coordinates: 2242655..2242969 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K29_RS10240	2237408..2237869	-	462	WP_124304701.1	hypothetical protein	-
C4K29_RS10245	2238421..2239242	+	822	WP_124304702.1	hypothetical protein	-
C4K29_RS10250	2239282..2240007	+	726	WP_124304703.1	hypothetical protein	-
C4K29_RS10255	2240301..2240729	-	429	WP_124303174.1	Arc family DNA-binding protein	-
C4K29_RS10260	2240847..2241002	+	156	WP_124303173.1	Arc family DNA-binding protein	-
C4K29_RS10265	2241075..2241383	+	309	WP_124304704.1	DUF3077 domain-containing protein	-
C4K29_RS10270	2241383..2242180	+	798	WP_124304705.1	phage antirepressor KilAC domain-containing protein	-
C4K29_RS10275	2242314..2242658	+	345	WP_062823026.1	hypothetical protein	Toxin
C4K29_RS10280	2242655..2242969	+	315	WP_124304706.1	transcriptional regulator	Antitoxin
C4K29_RS10285	2243021..2243440	-	420	WP_206430670.1	hypothetical protein	-
C4K29_RS10290	2243545..2243790	+	246	WP_124304708.1	helix-turn-helix domain-containing protein	-
C4K29_RS10295	2243914..2244336	+	423	WP_102680186.1	Rha family transcriptional regulator	-
C4K29_RS10300	2244436..2245098	+	663	WP_124304709.1	hypothetical protein	-
C4K29_RS10305	2245095..2245358	+	264	WP_124304710.1	hypothetical protein	-
C4K29_RS10315	2245573..2245998	+	426	WP_124304712.1	GIY-YIG nuclease family protein	-
C4K29_RS10320	2245995..2246789	+	795	WP_124304713.1	helix-turn-helix domain-containing protein	-
C4K29_RS10325	2246770..2247576	+	807	WP_164485753.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2241383..2292245	50862

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-94)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13208.96 Da
• Isoelectric Point: 9.6391

>T100111 WP_062823026.1 NZ_CP027740:2242314-2242658 [Pseudomonas chlororaphis subsp. piscium]
MKTVFFETTSFTATVGDYLTDDEYRQLQAEMQANPTAGDVMPRTGGFRKLRWHDSRRGKGKRGGLRVIYYWLLDNDQFWM
FSIYDKDEVTKLTSDQEKALKAAIDAELKKRGGK

Nucleotide

• Length: 345 bp

>T100111 NZ_CP027740:2242314-2242658 [Pseudomonas chlororaphis subsp. piscium]
ATGAAAACAGTCTTCTTTGAAACTACAAGCTTTACCGCCACGGTAGGCGACTATCTGACTGACGATGAATATCGGCAGCT
TCAAGCGGAGATGCAGGCGAACCCTACGGCTGGCGATGTGATGCCGCGTACGGGGGGATTCAGAAAGCTTCGTTGGCATG
ACTCCCGTAGAGGAAAGGGGAAGCGTGGTGGGCTTCGCGTCATCTACTACTGGCTGCTTGATAACGATCAGTTTTGGATG
TTTTCGATCTACGACAAGGACGAGGTTACAAAGCTGACTTCCGATCAGGAGAAGGCACTGAAAGCTGCGATAGATGCAGA
GCTGAAAAAGCGAGGTGGGAAATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12044.07 Da
• Isoelectric Point: 10.0926

>AT100111 WP_124304706.1 NZ_CP027740:2242655-2242969 [Pseudomonas chlororaphis subsp. piscium]
MKKRDLFAELMQGVEEMGEHRKGKITLRQYEMEPLPAPEVTAAEIISIREKLHMSQPVFARQIRTSPDTLKNWEQSKSKP
NAQAALLIKLVQRFPDMVERLNTV

Nucleotide

• Length: 315 bp

>AT100111 NZ_CP027740:2242655-2242969 [Pseudomonas chlororaphis subsp. piscium]
ATGAAAAAGCGCGATCTATTTGCTGAGTTGATGCAGGGTGTGGAAGAGATGGGCGAGCACCGGAAGGGGAAGATCACTCT
TCGTCAGTACGAGATGGAACCTCTCCCTGCACCAGAGGTGACTGCAGCTGAAATCATTTCTATCCGTGAAAAGCTTCATA
TGTCGCAGCCAGTGTTCGCCAGGCAAATTCGTACCAGTCCGGACACGCTGAAAAATTGGGAGCAGTCGAAGTCAAAGCCG
AATGCCCAGGCAGCATTGCTGATTAAGCTGGTTCAGCGTTTCCCTGATATGGTCGAGCGTCTTAACACTGTTTGA

Structures

Toxin

• AlphaFold DB: A0A7D6EBU8