Detailed information of TA system TA100075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 3293103..3293757
• Replicon: chromosome
• Accession: NZ_CP027734
• Organism: Pseudomonas sp. R1-43-08

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C4J87_RS15035
• Protein ID: WP_124367424.1
• Coordinates: 3293103..3293453 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C4J87_RS15040
• Protein ID: WP_124367425.1
• Coordinates: 3293443..3293757 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J87_RS15025	3289863..3291395	+	1533	WP_025858974.1	NADH-quinone oxidoreductase subunit M	-
C4J87_RS15030	3291403..3292866	+	1464	WP_124367423.1	NADH-quinone oxidoreductase subunit NuoN	-
C4J87_RS15035	3293103..3293453	+	351	WP_124367424.1	toxin	Toxin
C4J87_RS15040	3293443..3293757	+	315	WP_124367425.1	transcriptional regulator	Antitoxin
C4J87_RS15045	3293821..3294573	-	753	WP_124367426.1	outer membrane beta-barrel protein	-
C4J87_RS15050	3294826..3295062	+	237	WP_124363304.1	hypothetical protein	-
C4J87_RS15055	3295106..3295303	-	198	WP_124367427.1	hypothetical protein	-
C4J87_RS15060	3295318..3295497	-	180	WP_124367428.1	hypothetical protein	-
C4J87_RS15065	3295612..3296121	+	510	WP_124368982.1	DUF3087 family protein	-
C4J87_RS15070	3296116..3297489	-	1374	WP_124367429.1	MFS transporter	-
C4J87_RS15075	3297622..3298011	-	390	WP_124367430.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-100)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13725.61 Da
• Isoelectric Point: 9.7551

>T100075 WP_124367424.1 NZ_CP027734:3293103-3293453 [Pseudomonas sp. R1-43-08]
MDALFIELPAFERHRRDYLSDELFHGFQQELMKNPEAGDVIEGTGGLRKVRFVDERRNKGKRGGLRVIYYWWSGGTQFWL
FTLYGKHEQSDLAPHHKKALKHMLDREIKARAHNET

Nucleotide

• Length: 351 bp

>T100075 NZ_CP027734:3293103-3293453 [Pseudomonas sp. R1-43-08]
ATGGATGCTCTATTTATCGAACTGCCGGCATTCGAACGGCATCGCAGGGACTATCTGAGTGACGAGCTTTTCCACGGTTT
TCAACAGGAGCTGATGAAAAATCCGGAAGCGGGAGACGTGATCGAAGGAACGGGAGGGTTACGTAAGGTTCGCTTCGTCG
ATGAGCGACGAAACAAAGGCAAGCGCGGTGGCCTGCGGGTCATCTATTACTGGTGGTCGGGCGGCACGCAATTCTGGTTA
TTCACCTTGTACGGCAAACACGAACAGAGCGACCTGGCACCACACCACAAGAAAGCCCTTAAACACATGCTGGACAGGGA
AATCAAAGCGAGAGCACACAATGAAACGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11770.63 Da
• Isoelectric Point: 10.6525

>AT100075 WP_124367425.1 NZ_CP027734:3293443-3293757 [Pseudomonas sp. R1-43-08]
MKREIFSELVEGFDALADERQGKITLRTHKVHLNKLAPLTAEEVIAVRQQLNLSRSVFAMYLRTNTRTLENWEQGRAKPN
AQAVTLIRLVARFPETVAQLAALG

Nucleotide

• Length: 315 bp

>AT100075 NZ_CP027734:3293443-3293757 [Pseudomonas sp. R1-43-08]
ATGAAACGTGAAATTTTTTCCGAACTGGTCGAAGGCTTTGACGCCCTGGCCGACGAACGCCAGGGCAAAATCACCCTGCG
CACCCATAAAGTCCACCTCAACAAACTGGCGCCGCTCACCGCCGAAGAAGTGATCGCAGTGCGCCAGCAACTCAACCTCT
CCCGCTCGGTATTCGCCATGTACCTGCGCACCAATACCCGCACCCTGGAGAATTGGGAACAGGGCCGCGCCAAGCCCAAT
GCACAGGCGGTCACGCTGATACGCCTGGTGGCGCGTTTTCCTGAAACCGTTGCGCAACTTGCCGCGCTGGGCTGA