Detailed information of TA system TA100019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 3518606..3519210
• Replicon: chromosome
• Accession: NZ_CP027726
• Organism: Pseudomonas orientalis strain R4-35-08

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C4J95_RS15995
• Protein ID: WP_124358846.1
• Coordinates: 3518606..3518920 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A3G7XXD5
• Locus tag: C4J95_RS16000
• Protein ID: WP_065885023.1
• Coordinates: 3518923..3519210 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J95_RS15970	3513959..3514723	+	765	WP_124358841.1	SDR family oxidoreductase	-
C4J95_RS15975	3514733..3515533	+	801	WP_124358842.1	SDR family oxidoreductase	-
C4J95_RS15980	3515579..3516532	+	954	WP_124358843.1	sugar-binding transcriptional regulator	-
C4J95_RS15985	3516556..3517479	+	924	WP_124358844.1	transaldolase	-
C4J95_RS15990	3517677..3518381	-	705	WP_124358845.1	aquaporin Z	-
C4J95_RS15995	3518606..3518920	+	315	WP_124358846.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4J95_RS16000	3518923..3519210	+	288	WP_065885023.1	helix-turn-helix domain-containing protein	Antitoxin
C4J95_RS16005	3519240..3520424	-	1185	WP_124358847.1	sugar transporter	-
C4J95_RS16010	3520597..3521595	-	999	WP_124358848.1	bile acid:sodium symporter	-
C4J95_RS16015	3521680..3522468	+	789	WP_124358849.1	helix-turn-helix transcriptional regulator	-
C4J95_RS16020	3522566..3522997	+	432	WP_124358850.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11823.82 Da
• Isoelectric Point: 10.4492

>T100019 WP_124358846.1 NZ_CP027726:3518606-3518920 [Pseudomonas orientalis]
MIFIETAVFTRRVTELMNHDAYLGLQVALMLNPRAGDVIEGTGGIRKLRIASKGHGKRGGARVIYYHFVTASKIALLMIY
PKNEQQDLTNDERKTLKAVIEHWR

Nucleotide

• Length: 315 bp

>T100019 NZ_CP027726:3518606-3518920 [Pseudomonas orientalis]
ATGATTTTTATTGAAACCGCAGTTTTCACCCGGCGCGTAACGGAGTTGATGAATCACGATGCCTATCTGGGTTTGCAGGT
AGCGTTGATGTTGAATCCCCGCGCTGGAGATGTGATCGAAGGAACCGGCGGCATCCGAAAGCTGCGCATCGCCTCAAAAG
GTCACGGCAAACGAGGCGGTGCCAGGGTGATTTACTATCACTTTGTAACGGCTTCAAAAATCGCGTTATTGATGATCTAT
CCAAAAAACGAACAGCAAGACCTGACAAACGATGAACGCAAAACACTGAAAGCCGTCATCGAACACTGGAGATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10783.50 Da
• Isoelectric Point: 10.4748

>AT100019 WP_065885023.1 NZ_CP027726:3518923-3519210 [Pseudomonas orientalis]
MSKFFDELMESVQQMDEIVRGERQPSRQFIVDALQVKKIRKTTGLTQAKFAAMIDVQIGTLRNWEQGRREPTGPAKALLR
AIHNDPVHVLNALAP

Nucleotide

• Length: 288 bp

>AT100019 NZ_CP027726:3518923-3519210 [Pseudomonas orientalis]
ATGAGCAAATTCTTCGATGAGCTGATGGAAAGCGTGCAACAGATGGACGAAATCGTCCGAGGCGAGCGCCAGCCCTCTCG
CCAATTTATTGTTGACGCGCTTCAAGTCAAAAAAATTCGTAAGACAACAGGCCTGACTCAAGCCAAATTTGCCGCAATGA
TCGACGTTCAGATTGGCACACTGCGCAATTGGGAACAGGGGCGACGCGAACCGACGGGCCCAGCAAAAGCGCTGTTGAGG
GCCATTCACAACGACCCTGTACATGTGTTGAATGCGTTGGCGCCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A3G7XXD5