Detailed information of TA system TA100010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 3342543..3343060
• Replicon: chromosome
• Accession: NZ_CP027725
• Organism: Pseudomonas orientalis strain R2-66-08W

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C4J96_RS14850
• Protein ID: WP_164486809.1
• Coordinates: 3342543..3342770 (+)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A3G7XXD5
• Locus tag: C4J96_RS14855
• Protein ID: WP_065885023.1
• Coordinates: 3342773..3343060 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J96_RS14825	3337844..3338608	+	765	WP_124358841.1	SDR family oxidoreductase	-
C4J96_RS14830	3338618..3339418	+	801	WP_124358842.1	SDR family oxidoreductase	-
C4J96_RS14835	3339464..3340417	+	954	WP_124358843.1	sugar-binding transcriptional regulator	-
C4J96_RS14840	3340441..3341364	+	924	WP_124358844.1	transaldolase	-
C4J96_RS14845	3341562..3342266	-	705	WP_124358845.1	aquaporin Z	-
C4J96_RS14850	3342543..3342770	+	228	WP_164486809.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4J96_RS14855	3342773..3343060	+	288	WP_065885023.1	helix-turn-helix domain-containing protein	Antitoxin
C4J96_RS14860	3343090..3344274	-	1185	WP_124358847.1	sugar transporter	-
C4J96_RS14865	3344447..3345445	-	999	WP_124358848.1	bile acid:sodium symporter	-
C4J96_RS14870	3345530..3346318	+	789	WP_124426900.1	helix-turn-helix transcriptional regulator	-
C4J96_RS14875	3346416..3346847	+	432	WP_124426901.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (14-73)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8488.87 Da
• Isoelectric Point: 10.7741

>T100010 WP_164486809.1 NZ_CP027725:3342543-3342770 [Pseudomonas orientalis]
MLNPRAGDVIEGTGGIRKLRIASKGHGKRGGARVIYYHFVTASKIALLMIYPKNEQQDLTNDERKTLKAVIEHWR

Nucleotide

• Length: 228 bp

>T100010 NZ_CP027725:3342543-3342770 [Pseudomonas orientalis]
ATGTTGAATCCCCGCGCTGGAGATGTGATCGAAGGAACCGGCGGCATCCGAAAGCTGCGCATCGCCTCAAAAGGTCACGG
CAAACGAGGCGGTGCCAGGGTGATTTACTATCACTTTGTAACGGCTTCAAAAATCGCGTTATTGATGATCTATCCAAAAA
ACGAACAGCAAGACCTGACAAACGATGAACGCAAAACACTGAAAGCCGTCATCGAACACTGGAGATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10783.50 Da
• Isoelectric Point: 10.4748

>AT100010 WP_065885023.1 NZ_CP027725:3342773-3343060 [Pseudomonas orientalis]
MSKFFDELMESVQQMDEIVRGERQPSRQFIVDALQVKKIRKTTGLTQAKFAAMIDVQIGTLRNWEQGRREPTGPAKALLR
AIHNDPVHVLNALAP

Nucleotide

• Length: 288 bp

>AT100010 NZ_CP027725:3342773-3343060 [Pseudomonas orientalis]
ATGAGCAAATTCTTCGATGAGCTGATGGAAAGCGTGCAACAGATGGACGAAATCGTCCGAGGCGAGCGCCAGCCCTCTCG
CCAATTTATTGTTGACGCGCTTCAAGTCAAAAAAATTCGTAAGACAACAGGCCTGACTCAAGCCAAATTTGCCGCAATGA
TCGACGTTCAGATTGGCACACTGCGCAATTGGGAACAGGGGCGACGCGAACCGACGGGCCCAGCAAAGGCGCTGTTGAGG
GCCATTCACAACGACCCTGTACATGTGTTGAATGCGTTGGCGCCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A3G7XXD5