MGE detailed information
MGE type: Conjugative plasmid MGE length: 140210 bp
Accession: NZ_CP103541 Location: 1..140210 Organism: Escherichia coli strain 5264 Replicon: plasmid pMB9081_1
Accession: NZ_CP103541 Location: 1..140210 Organism: Escherichia coli strain 5264 Replicon: plasmid pMB9081_1
Gene structure
The 5 kb flanking regions of this MGE are displayed.
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| M5S66_RS22880 (1) | 1..756 | + | 756 | WP_000852146.1 | replication initiation protein RepE | - |
| M5S66_RS22885 (1344) | 1344..2510 | + | 1167 | WP_000772446.1 | hypothetical protein | VF |
| M5S66_RS22890 (2510) | 2510..3481 | + | 972 | WP_000817036.1 | ParB/RepB/Spo0J family plasmid partition protein | - |
| M5S66_RS22895 (4348) | 4348..5250 | + | 903 | WP_001507408.1 | DUF1281 domain-containing protein | - |
| M5S66_RS22900 (5247) | 5247..5558 | + | 312 | WP_032260558.1 | hypothetical protein | - |
| M5S66_RS22905 (5634) | 5634..6317 | + | 684 | WP_000086148.1 | DNA methylase | - |
| M5S66_RS22910 (6318) | 6318..6539 | + | 222 | WP_001104867.1 | hypothetical protein | - |
| M5S66_RS22915 (6520) | 6520..6987 | + | 468 | WP_223368614.1 | DUF1380 family protein | - |
| M5S66_RS22920 (7038) | 7038..7814 | + | 777 | WP_001753849.1 | hypothetical protein | - |
| M5S66_RS22925 (8231) | 8231..8656 | + | 426 | WP_001198941.1 | antirestriction protein | - |
| M5S66_RS22930 (8626) | 8626..9117 | + | 492 | WP_223368613.1 | DUF1380 domain-containing protein | - |
| M5S66_RS22935 (9117) | 9117..9308 | + | 192 | WP_001027495.1 | hypothetical protein | - |
| M5S66_RS22940 (9670) | 9670..9820 | - | 151 | Protein_12 | hypothetical protein | - |
| M5S66_RS22945 (10337) | 10337..10567 | + | 231 | WP_000218642.1 | hypothetical protein | - |
| M5S66_RS22950 (10619) | 10619..11980 | + | 1362 | WP_086352864.1 | DUF3560 domain-containing protein | - |
| M5S66_RS22955 (12028) | 12028..12591 | + | 564 | WP_029702196.1 | class I SAM-dependent methyltransferase | - |
| M5S66_RS22960 (12677) | 12677..12847 | - | 171 | Protein_16 | hypothetical protein | - |
| M5S66_RS22965 (12960) | 12960..13823 | - | 864 | WP_259412412.1 | group II intron maturase-specific domain-containing protein | - |
| M5S66_RS22975 (15056) | 15056..15940 | - | 885 | Protein_19 | reverse transcriptase domain-containing protein | - |
| M5S66_RS22980 (17602) | 17602..18171 | - | 570 | WP_089634947.1 | hypothetical protein | - |
| M5S66_RS22990 (19475) | 19475..20233 | + | 759 | Protein_22 | site-specific integrase | - |
| M5S66_RS22995 (20391) | 20391..21401 | + | 1011 | WP_000053332.1 | substrate-binding domain-containing protein | - |
| M5S66_RS23000 (21497) | 21497..23623 | + | 2127 | WP_000253905.1 | alpha-galactosidase | - |
| M5S66_RS23005 (23686) | 23686..24963 | + | 1278 | WP_001083369.1 | MFS transporter | - |
| M5S66_RS23010 (24960) | 24960..26393 | + | 1434 | WP_024170639.1 | glycoside hydrolase family 32 protein | - |
| M5S66_RS23015 (26588) | 26588..27982 | + | 1395 | WP_001037799.1 | carbohydrate porin | - |
| M5S66_RS23020 (28228) | 28228..28332 | - | 105 | Protein_28 | thiosulfate reductase cytochrome B subunit | - |
| M5S66_RS23025 (28389) | 28389..29082 | + | 694 | Protein_29 | IS1 family transposase | - |
| M5S66_RS23030 (29209) | 29209..29721 | + | 513 | WP_223350513.1 | potassium channel family protein | - |
| M5S66_RS23035 (29806) | 29806..30057 | + | 252 | Protein_31 | iron-containing alcohol dehydrogenase | - |
| M5S66_RS23040 (30346) | 30346..30663 | - | 318 | Protein_32 | IS110 family transposase IS1618 | Transposase |
| M5S66_RS23045 (30818) | 30818..30923 | + | 106 | Protein_33 | plasmid SOS inhibition protein A | - |
| - (30892) | 30892..31082 | + | 191 | NuclAT_0 | - | - |
| - (30892) | 30892..31082 | + | 191 | NuclAT_0 | - | - |
| - (30892) | 30892..31082 | + | 191 | NuclAT_0 | - | - |
| - (30892) | 30892..31082 | + | 191 | NuclAT_0 | - | - |
| M5S66_RS23050 (31132) | 31132..32501 | + | 1370 | WP_085947770.1 | IS3 family transposase IS150 | Transposase |
| M5S66_RS23055 (32543) | 32543..32692 | + | 150 | Protein_35 | DUF5431 family protein | - |
| M5S66_RS23060 (32643) | 32643..32759 | + | 117 | WP_001375150.1 | type I toxin-antitoxin system Hok family toxin | - |
| M5S66_RS23065 (33060) | 33060..33357 | - | 298 | Protein_37 | hypothetical protein | - |
| M5S66_RS23070 (33427) | 33427..33633 | + | 207 | WP_000275854.1 | hypothetical protein | - |
| M5S66_RS23075 (33658) | 33658..33945 | + | 288 | WP_000107242.1 | hypothetical protein | - |
| M5S66_RS23080 (34064) | 34064..34885 | + | 822 | WP_001234465.1 | DUF932 domain-containing protein | - |
| M5S66_RS23085 (35181) | 35181..35753 | - | 573 | WP_000949660.1 | hypothetical protein | VF |
| M5S66_RS23090 (36123) | 36123..36506 | + | 384 | WP_001063020.1 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| M5S66_RS23095 (36697) | 36697..37344 | + | 648 | WP_000332520.1 | conjugal transfer transcriptional regulator TraJ | - |
| M5S66_RS23100 (37480) | 37480..37695 | + | 216 | WP_001352842.1 | conjugal transfer relaxosome protein TraY | - |
| M5S66_RS23105 (37738) | 37738..38103 | + | 366 | WP_000340282.1 | type IV conjugative transfer system pilin TraA | - |
| M5S66_RS23110 (38118) | 38118..38429 | + | 312 | WP_000012106.1 | type IV conjugative transfer system protein TraL | - |
| M5S66_RS23115 (38451) | 38451..39017 | + | 567 | WP_000399790.1 | type IV conjugative transfer system protein TraE | - |
| M5S66_RS23120 (39004) | 39004..39732 | + | 729 | WP_001230813.1 | type-F conjugative transfer system secretin TraK | - |
| M5S66_RS23125 (39732) | 39732..41159 | + | 1428 | WP_001553836.1 | F-type conjugal transfer pilus assembly protein TraB | - |
| M5S66_RS23130 (41149) | 41149..41739 | + | 591 | WP_000002787.1 | conjugal transfer pilus-stabilizing protein TraP | - |
| M5S66_RS23135 (41726) | 41726..42046 | + | 321 | Protein_51 | conjugal transfer protein TrbD | - |
| M5S66_RS23140 (42039) | 42039..42290 | + | 252 | WP_001038342.1 | conjugal transfer protein TrbG | - |
| M5S66_RS23145 (42287) | 42287..42802 | + | 516 | WP_000809832.1 | type IV conjugative transfer system lipoprotein TraV | - |
| M5S66_RS23150 (42937) | 42937..43158 | + | 222 | WP_001278692.1 | conjugal transfer protein TraR | - |
| M5S66_RS23155 (43318) | 43318..45945 | + | 2628 | WP_039021964.1 | type IV secretion system protein TraC | - |
| M5S66_RS23160 (45942) | 45942..46328 | + | 387 | WP_000214092.1 | type-F conjugative transfer system protein TrbI | - |
| M5S66_RS23165 (46325) | 46325..46957 | + | 633 | WP_001203720.1 | type-F conjugative transfer system protein TraW | - |
| M5S66_RS23170 (46954) | 46954..47946 | + | 993 | WP_000830839.1 | conjugal transfer pilus assembly protein TraU | - |
| M5S66_RS23175 (47955) | 47955..48593 | + | 639 | WP_000777692.1 | type-F conjugative transfer system pilin assembly protein TrbC | - |
| M5S66_RS23180 (48590) | 48590..50398 | + | 1809 | WP_000821821.1 | type-F conjugative transfer system mating-pair stabilization protein TraN | - |
| M5S66_RS23185 (50425) | 50425..50682 | + | 258 | WP_000864352.1 | conjugal transfer protein TrbE | - |
| M5S66_RS23190 (50675) | 50675..51418 | + | 744 | WP_001430248.1 | type-F conjugative transfer system pilin assembly protein TraF | - |
| M5S66_RS23195 (51432) | 51432..51773 | + | 342 | WP_000556794.1 | conjugal transfer protein TrbA | - |
| M5S66_RS23200 (51754) | 51754..52089 | - | 336 | WP_000415571.1 | hypothetical protein | - |
| M5S66_RS23205 (52170) | 52170..52454 | + | 285 | WP_000624105.1 | type-F conjugative transfer system pilin chaperone TraQ | - |
| M5S66_RS23210 (52441) | 52441..52995 | + | 555 | WP_001553830.1 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | - |
| M5S66_RS23215 (52925) | 52925..53298 | + | 374 | Protein_67 | P-type conjugative transfer protein TrbJ | - |
| M5S66_RS23220 (53252) | 53252..53644 | + | 393 | WP_001553828.1 | F-type conjugal transfer protein TrbF | - |
| M5S66_RS23225 (53631) | 53631..55004 | + | 1374 | WP_001553827.1 | conjugal transfer pilus assembly protein TraH | - |
| M5S66_RS23230 (55001) | 55001..57826 | + | 2826 | WP_001553826.1 | conjugal transfer mating-pair stabilization protein TraG | - |
| M5S66_RS23235 (57823) | 57823..58332 | + | 510 | WP_000628100.1 | conjugal transfer entry exclusion protein TraS | - |
| M5S66_RS23240 (58346) | 58346..59077 | + | 732 | WP_000850424.1 | conjugal transfer complement resistance protein TraT | - |
| M5S66_RS23245 (59329) | 59329..61518 | + | 2190 | WP_040110343.1 | type IV conjugative transfer system coupling protein TraD | - |
| M5S66_RS23250 (61518) | 61518..66638 | + | 5121 | WP_013362805.1 | Multifunctional conjugation protein TraI | Relaxase |
| M5S66_RS23255 (66658) | 66658..67404 | + | 747 | WP_187795572.1 | conjugal transfer pilus acetylase TraX | - |
| M5S66_RS23260 (67459) | 67459..68019 | + | 561 | WP_000139363.1 | fertility inhibition protein FinO | - |
| M5S66_RS23265 (68153) | 68153..68365 | + | 213 | WP_005012601.1 | hypothetical protein | - |
| M5S66_RS23270 (68666) | 68666..68755 | - | 90 | Protein_78 | IS1 family transposase | - |
| M5S66_RS23275 (68810) | 68810..69487 | + | 678 | WP_001339397.1 | IS66 family transposase ISCro1 | Transposase |
| M5S66_RS23280 (69487) | 69487..69834 | + | 348 | WP_000624622.1 | IS66 family transposase ISCro1 | Transposase |
| M5S66_RS23285 (69854) | 69854..71425 | + | 1572 | WP_023149734.1 | IS66 family transposase ISCro1 | Transposase |
| M5S66_RS23290 (71463) | 71463..72079 | - | 617 | Protein_82 | IS1-like element IS1A family transposase | - |
| M5S66_RS23295 (72180) | 72180..72362 | + | 183 | WP_000968309.1 | hypothetical protein | - |
| - (72501) | 72501..72562 | - | 62 | NuclAT_1 | - | - |
| - (72501) | 72501..72562 | - | 62 | NuclAT_1 | - | - |
| - (72501) | 72501..72562 | - | 62 | NuclAT_1 | - | - |
| - (72501) | 72501..72562 | - | 62 | NuclAT_1 | - | - |
| M5S66_RS23300 (72606) | 72606..72755 | + | 150 | WP_001312851.1 | Hok/Gef family protein | - |
| M5S66_RS23305 (73039) | 73039..73296 | + | 258 | WP_000083833.1 | replication regulatory protein RepA | - |
| M5S66_RS23310 (73532) | 73532..73606 | + | 75 | WP_032336874.1 | RepA leader peptide Tap | - |
| M5S66_RS23315 (73599) | 73599..74045 | + | 447 | Protein_87 | plasmid replication initiator RepA | - |
| M5S66_RS23320 (74045) | 74045..74659 | - | 615 | Protein_88 | VENN motif pre-toxin domain-containing protein | - |
| M5S66_RS23325 (75366) | 75366..76586 | + | 1221 | WP_000410951.1 | arginine deiminase | - |
| M5S66_RS23330 (76597) | 76597..77508 | + | 912 | WP_000440183.1 | carbamate kinase | - |
| M5S66_RS23335 (77593) | 77593..78597 | + | 1005 | WP_000154545.1 | Ornithine carbamoyltransferase | VF |
| M5S66_RS23340 (78645) | 78645..80048 | + | 1404 | WP_000514417.1 | YfcC family protein | - |
| M5S66_RS23345 (80129) | 80129..80608 | + | 480 | WP_001496175.1 | ArgR family transcriptional regulator | - |
| M5S66_RS23350 (80672) | 80672..80860 | - | 189 | Protein_94 | transposase domain-containing protein | - |
| M5S66_RS23355 (80921) | 80921..82054 | + | 1134 | WP_000555401.1 | IS110 family transposase ISEc45 | Transposase |
| M5S66_RS23360 (82308) | 82308..82460 | - | 153 | Protein_96 | transposase | - |
| M5S66_RS23365 (82463) | 82463..82633 | - | 171 | Protein_97 | IS66 family transposase ISEc43 | Transposase |
| M5S66_RS23370 (82664) | 82664..83014 | - | 351 | WP_000624725.1 | IS66 family transposase ISEc47 | Transposase |
| M5S66_RS23375 (83011) | 83011..83367 | - | 357 | WP_000229735.1 | IS66 family transposase ISEc23 | Transposase |
| M5S66_RS23385 (84212) | 84212..84790 | + | 579 | WP_032152936.1 | DUF2726 domain-containing protein | - |
| M5S66_RS23390 (85203) | 85203..85556 | - | 354 | WP_000005489.1 | hypothetical protein | - |
| M5S66_RS23395 (86028) | 86028..87050 | - | 1023 | WP_000156883.1 | IS110 family transposase ISSm1 | Transposase |
| M5S66_RS23400 (87455) | 87455..87712 | + | 258 | WP_000083821.1 | replication regulatory protein RepA | - |
| M5S66_RS23405 (87947) | 87947..88021 | + | 75 | WP_001365705.1 | RepA leader peptide Tap | - |
| M5S66_RS23410 (88014) | 88014..88871 | + | 858 | WP_032152935.1 | incFII family plasmid replication initiator RepA | - |
| M5S66_RS23415 (89810) | 89810..90463 | + | 654 | WP_000616807.1 | CPBP family intramembrane metalloprotease | - |
| M5S66_RS23420 (90556) | 90556..90813 | + | 258 | WP_000557619.1 | type II toxin-antitoxin system antitoxin PemI | - |
| M5S66_RS23425 (90746) | 90746..91147 | + | 402 | WP_001398199.1 | type II toxin-antitoxin system toxin endoribonuclease PemK | - |
| M5S66_RS23430 (91284) | 91284..94181 | - | 2898 | WP_001553819.1 | Tn3 family transposase TnpA_Tn5403 | Transposase |
| M5S66_RS23435 (94276) | 94276..94881 | + | 606 | WP_000509965.1 | Tn3 family transposase TnpR_Tn5403 | Transposase |
| M5S66_RS23440 (94905) | 94905..95654 | - | 750 | Protein_112 | Tn3 family transposase TnpA_TnAs1 | Transposase |
| M5S66_RS23445 (95658) | 95658..96050 | + | 393 | WP_001351729.1 | isochorismatase family cysteine hydrolase | - |
| M5S66_RS23450 (96188) | 96188..97072 | + | 885 | WP_000058717.1 | EamA family transporter | - |
| M5S66_RS23455 (97104) | 97104..98303 | - | 1200 | WP_000804064.1 | Tetracycline resistance protein, class C | ARG |
| M5S66_RS23460 (98409) | 98409..99059 | + | 651 | WP_000164043.1 | tetracycline resistance transcriptional repressor TetR(A) | - |
| M5S66_RS23465 (99091) | 99091..99333 | - | 243 | WP_032140899.1 | hypothetical protein | - |
| M5S66_RS23470 (99375) | 99375..100889 | - | 1515 | Protein_118 | Tn3 family transposase TnpA_TnAs1 | Transposase |
| M5S66_RS23475 (100955) | 100955..101659 | + | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| M5S66_RS23480 (101803) | 101803..102357 | + | 555 | WP_063840321.1 | Aminoglycoside N(6')-acetyltransferase type 1 | ARG |
| M5S66_RS23485 (102488) | 102488..103318 | + | 831 | WP_001334766.1 | Beta-lactamase OXA-1 | ARG |
| M5S66_RS23490 (103456) | 103456..103896 | + | 441 | Protein_122 | CatB-related O-acetyltransferase | - |
| M5S66_RS23495 (103950) | 103950..104654 | - | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| M5S66_RS23500 (104720) | 104720..105007 | + | 288 | Protein_124 | IS1380 family transposase ISEcp1 | Transposase |
| M5S66_RS23505 (105263) | 105263..106138 | + | 876 | WP_000239590.1 | Beta-lactamase CTX-M-1 | ARG |
| M5S66_RS23510 (106185) | 106185..106478 | - | 294 | WP_116961751.1 | WbuC family cupin fold metalloprotein | - |
| M5S66_RS23515 (106543) | 106543..108762 | + | 2220 | Protein_127 | Tn3 family transposase TnpA_Tn3 | Transposase |
| M5S66_RS23520 (108839) | 108839..109543 | + | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| M5S66_RS23525 (109613) | 109613..109900 | + | 288 | Protein_129 | transposase | - |
| M5S66_RS23530 (110393) | 110393..110977 | - | 585 | WP_001137892.1 | macrolide-binding transcriptional repressor MphR(A) | - |
| M5S66_RS23535 (110977) | 110977..112215 | - | 1239 | WP_000004159.1 | macrolide resistance MFS transporter Mrx(A) | - |
| M5S66_RS23540 (112212) | 112212..113126 | - | 915 | WP_023063803.1 | hypothetical protein | ARG |
| M5S66_RS23545 (113248) | 113248..113952 | - | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| M5S66_RS23550 (114117) | 114117..114410 | + | 294 | Protein_134 | IS30 family transposase ISAba125 | Transposase |
| M5S66_RS23555 (114511) | 114511..115323 | + | 813 | WP_023408309.1 | Metallo-beta-lactamase type 2 | ARG |
| M5S66_RS23560 (115327) | 115327..115692 | + | 366 | WP_004201167.1 | bleomycin binding protein Ble-MBL | - |
| M5S66_RS23565 (115697) | 115697..116335 | + | 639 | WP_004201168.1 | phosphoribosylanthranilate isomerase | - |
| M5S66_RS23570 (116346) | 116346..116903 | - | 558 | Protein_138 | twin-arginine translocation (TAT) pathway signal sequence domain protein | - |
| M5S66_RS23575 (117003) | 117003..117377 | - | 375 | Protein_139 | protein-disulfide reductase DsbD family protein | - |
| M5S66_RS23580 (117690) | 117690..119231 | - | 1542 | WP_000050481.1 | IS91 family transposase ISVsa3 | Transposase |
| M5S66_RS23585 (119636) | 119636..120475 | - | 840 | WP_000259031.1 | Dihydropteroate synthase | ARG |
| M5S66_RS23590 (120469) | 120469..120816 | - | 348 | WP_000679427.1 | Multidrug transporter EmrE | ARG |
| M5S66_RS23595 (120933) | 120933..121771 | - | 839 | Protein_143 | Streptomycin 3''-adenylyltransferase | ARG |
| M5S66_RS23600 (121777) | 121777..122067 | - | 291 | WP_001336345.1 | DUF1010 domain-containing protein | - |
| M5S66_RS23605 (122179) | 122179..122676 | - | 498 | WP_001083725.1 | IS1595 family transposase ISSsu9 | ARG |
| M5S66_RS23610 (122821) | 122821..123687 | + | 867 | Protein_146 | IS91 family transposase ISAzo26 | Transposase |
| M5S66_RS23615 (123742) | 123742..124446 | - | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| M5S66_RS23620 (125001) | 125001..125285 | + | 285 | WP_001387467.1 | ribbon-helix-helix domain-containing protein | Antitoxin Detail |
| M5S66_RS23625 (125285) | 125285..125560 | + | 276 | WP_000421272.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin Detail |
| M5S66_RS23635 (126443) | 126443..126646 | + | 204 | Protein_151 | hypothetical protein | - |
| M5S66_RS23640 (126771) | 126771..126899 | - | 129 | Protein_152 | IS481 family transposase | - |
| M5S66_RS23645 (126932) | 126932..128101 | - | 1170 | WP_001072355.1 | hypothetical protein | - |
| M5S66_RS23650 (128633) | 128633..128901 | + | 269 | Protein_154 | type II toxin-antitoxin system ParD family antitoxin | - |
| M5S66_RS23655 (128888) | 128888..129177 | + | 290 | Protein_155 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| M5S66_RS23660 (129244) | 129244..129426 | + | 183 | WP_042344623.1 | hypothetical protein | - |
| M5S66_RS23665 (129547) | 129547..130287 | + | 741 | WP_001066953.1 | tyrosine-type recombinase/integrase | - |
| M5S66_RS23670 (130572) | 130572..131549 | - | 978 | WP_000361611.1 | RepB family plasmid replication initiator protein | - |
| M5S66_RS23675 (132306) | 132306..133278 | + | 973 | Protein_159 | hypothetical protein | - |
| M5S66_RS23680 (133437) | 133437..133604 | - | 168 | WP_259412432.1 | abortive infection family protein | - |
| M5S66_RS23685 (133743) | 133743..134440 | + | 698 | WP_224434100.1 | IS1-like element IS1B family transposase | - |
| M5S66_RS23690 (134569) | 134569..135266 | + | 698 | WP_224434100.1 | IS1-like element IS1B family transposase | - |
| M5S66_RS23695 (135466) | 135466..136398 | - | 933 | WP_058682609.1 | S-4TM family putative pore-forming effector | - |
| M5S66_RS23700 (136402) | 136402..137397 | - | 996 | WP_000246636.1 | hypothetical protein | - |
| M5S66_RS23705 (138105) | 138105..138323 | + | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| M5S66_RS23710 (138325) | 138325..138630 | + | 306 | WP_001159868.1 | type II toxin-antitoxin system toxin CcdB | - |
| M5S66_RS23715 (138631) | 138631..139437 | + | 807 | WP_000016982.1 | site-specific integrase | - |
Similar MGE(s)
Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.
| Detail | Organism | MGE type | Related TA | Genome accession | Coordinates | Mash distance |
|---|