| Effector Protein ID: 54294840 |
 T4SS name [ID] | Dot/Icm [41] |
| Gene symbol | ralF |
| Locus tag | lpl1919 |
| Strain | Legionella pneumophila str. Lens |
| Replicon | chromosome [GenBank: NC_006369] [Browse all T4SS(s) in this replicon] |
| Location [Strand] | 2154317..2155513 [+] |
| Product | RalF protein, translocated into host cells by the Dot/Icm system |
| Effector type | Protein |
| Descripton | RalF |
| T4CP requirement | T4CP Depedent |
| Target/Effection | RalF mediates Legionella-containing vacuole (LCV) recruitment of Arf1 (ADP-ribosylation factor 1), which is a GTPase responsible for mediating vesicular transport between Golgi and endoplastic reticulum. RalF colocalizes with Arf1 and activates it on the LCV after bacterial internalization |
| UniProt ID | Q5WV98 |
| KEGG ID | lpf:lpl1919 |
| PDB ID | NA |
| Domain hit(s) vs Pfam | Sec7 [PF01369], Evalue: 1.7e-56, Aligned region: 10..192 |
| Protein Sequence: 398 a.a. [Download] |
| >gi|54294840|ref|YP_127255.1| RalF protein, translocated into host cells by the Dot/Icm system [Legionella pneumophila str. Lens] MPSEIEKAQRDITEAFNAKPKNGIEKIKEICEENKISPNEEIAAFFHQQRKNLDLEAVGDYLSGPEAENQ QVLKAFTSQMDFNGQSFVEGLRTFLKTFKLPGEAQKIDRLVQSFSAAYSQQNPDVVSNADAAYLLAFQTI MLNTDLHNPSIPEKNKMTVDGLKRNLRGGNNGGDFDAKFLEELYSEIKAKPFELNFVKTSPGYELNSTTL NKDSTFKKLNSFLYSTDANINTVFPGIGDNVKTTVDQPKSWLSFFTGYKGTITLTDKQTSAQATIQVYTP NIFSKWLFGEQPRVIIQPGQTKESIDLAAKAAAGFSSPVKNFKATYDYEVGDLIKAYDNQKKLISPNKNM VIKGSIFRTPNAEQRETSKSATRTVNTDYDINEDTVTRKDESRSSYKK |
| - |
| Nucleotide Sequence: 1197 bp [Download] |
| >gi|54292964|ref|NC_006369.1|:2154317-2155513 Legionella pneumophila str. Lens, complete genome ATGCCTTCAGAAATTGAAAAGGCACAAAGAGATATTACTGAGGCTTTTAACGCCAAACCCAAAAATGGGA TAGAAAAAATAAAAGAAATATGTGAAGAAAATAAAATTTCTCCGAATGAAGAAATTGCCGCTTTCTTTCA TCAACAAAGAAAAAATTTGGATTTAGAAGCGGTTGGCGATTATTTGAGTGGGCCAGAAGCAGAAAACCAG CAAGTATTAAAAGCTTTTACCAGTCAAATGGATTTCAATGGGCAGAGTTTTGTTGAGGGTTTAAGGACCT TTTTGAAAACATTTAAATTACCAGGCGAAGCTCAAAAAATTGACAGGTTAGTACAAAGCTTTAGTGCTGC CTATTCCCAGCAAAATCCTGATGTGGTTTCCAATGCGGATGCTGCCTATCTTTTAGCATTTCAAACCATT ATGCTTAATACTGATTTACATAACCCAAGTATCCCTGAGAAAAATAAAATGACCGTGGATGGACTCAAAA GAAATCTCAGAGGAGGTAATAATGGTGGAGATTTTGATGCCAAATTTTTAGAGGAACTATACAGTGAGAT TAAAGCCAAACCTTTTGAGTTGAATTTCGTTAAAACCAGCCCAGGATATGAACTTAATTCAACCACTCTC AATAAAGATTCTACCTTTAAAAAACTAAATTCATTCTTGTATTCAACGGATGCAAACATCAATACAGTTT TTCCAGGAATAGGTGATAATGTTAAAACAACTGTAGATCAACCAAAATCATGGTTAAGTTTTTTTACCGG ATATAAGGGAACAATAACGTTAACGGATAAGCAAACGTCTGCACAAGCTACTATCCAGGTTTATACTCCC AATATATTTTCAAAATGGCTATTTGGAGAACAACCAAGAGTAATAATACAACCTGGACAAACCAAAGAGT CAATCGATTTGGCAGCAAAAGCAGCCGCTGGTTTTTCATCGCCAGTAAAAAATTTTAAAGCGACGTATGA TTATGAGGTAGGTGATTTAATAAAAGCATATGATAACCAGAAAAAACTCATTTCGCCCAATAAAAATATG GTAATTAAGGGGAGTATATTCAGAACACCAAACGCTGAACAACGAGAAACTTCAAAATCTGCAACAAGAA CGGTTAACACTGACTATGATATAAACGAGGATACAGTGACTAGAAAAGATGAAAGTAGATCCAGTTATAA GAAATAA |
| Reference |
(1) Gomez-Valero L; Rusniok C; Cazalet C; Buchrieser C (2011). Comparative and functional genomics of legionella identified eukaryotic like proteins as key players in host-pathogen interactions. Front Microbiol. 2:208. [PudMed:22059087] 
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| in silico analysis literature |