SecReT4
T4SS ID41
StrainLegionella pneumophila str. Lens
Repliconchromosome [Browse all T4SS(s) in this replicon]
AccessionNC_006369
Location526046..562439; 617992..618618; 2968136..2986785
NameDot/Icm
Functioneffector translocation
ClassificationType IVB; Type I
sequence This T4SS has been mentioned in a genome sequencing report.
structure Information of structure: structureAMP-PNP•LegK4, Apo-LegK4

T4SS components
ComponentDotADotBDotCDotDIcmBIcmCIcmDIcmEIcmF
Number111112111
ComponentIcmGIcmHIcmJIcmKIcmLIcmMIcmNIcmOIcmP
Number111111111
ComponentIcmQIcmRIcmSIcmTIcmVIcmWIcmXLvgA
Number11111111

The information of T4SS components from NC_006369
Region 1: 526046..562439
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1lpl0480521696..523705 [+], 201054293431hypothetical protein 
2lpl0481524521..525459 [-], 93954293432hypothetical protein 
3lpl0482525663..525875 [+], 21354293433hypothetical protein 
4lpl0483 (icmT)526046..526306 [+], 26154293434hypothetical protein  IcmT
5lpl0484 (icmS)526307..526651 [+], 34554293435hypothetical protein  IcmSaccesspry protein
6lpl0485 (icmR)526751..527113 [+], 36354293436hypothetical protein  IcmR
7lpl0486 (icmQ)527204..527779 [+], 57654293437hypothetical protein  IcmQ
8lpl0487 (icmP/dotM)527966..529096 [+], 113154293438hypothetical protein  IcmP
9lpl0488 (icmO/dotL)529093..531444 [+], 235254293439hypothetical protein  IcmO
10lpl0489 (lphA)531848..532417 [+], 57054293440hypothetical protein  IcmN
11lpl0490 (icmM/dotJ)532429..532713 [+], 28554293441hypothetical protein  IcmM
12lpl0491 (icmL/dotI)532729..533367 [+], 63954293442hypothetical protein  IcmL
13lpl0492 (icmK/dotH)533370..534452 [+], 108354293443hypothetical protein  IcmK
14lpl0493 (icmE/dotG)534457..537603 [+], 314754293444hypothetical protein  IcmE
15lpl0494 (icmG/dotF)537618..538427 [+], 81054293445hypothetical protein  IcmG
16lpl0495 (icmC/dotE)538435..539019 [+], 58554293446hypothetical protein  IcmC
17lpl0496 (icmD)539045..539443 [+], 39954293447hypothetical protein  IcmD
18lpl0497 (icmJ/dotN)539641..540267 [+], 62754293448hypothetical protein  IcmJ
19lpl0498 (icmB/dotO)540303..543332 [+], 303054293449hypothetical protein  IcmB
20lpl0499 (citA/tphA)543455..544711 [-], 125754293450proline/betaine transport protein 
21lpl0500 (icmF)544714..547635 [-], 292254293451hypothetical protein  IcmF
22lpl0501 (dotU)547635..548420 [-], 78654293452hypothetical protein  IcmH
23lpl0502 (purH)548705..550294 [-], 159054293453bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase 
24lpl0503 (prmA)550321..551190 [-], 87054293454ribosomal protein L11 methyltransferase 
25lpl0504 (accC)551192..552535 [-], 134454293455biotin carboxylase (A subunit of acetyl-CoA carboxylase) 
26lpl0505 (accB)552548..553030 [-], 48354293456acetyl-CoA carboxylase biotin carboxyl carrier protein 
27lpl0506 (aroQ)553044..553481 [-], 438542934573-dehydroquinate dehydratase 
28lpl0507 (dcoA)553683..555473 [+], 179154293458pyruvate carboxylase subunit B 
29lpl0508 (proA)556038..557669 [+], 163254293459zinc metalloproteinase precursor 
30lpl0509557671..558519 [-], 84954293460hypothetical protein 
31lpl0510558969..559742 [+], 77454293461hypothetical protein 
32lpl0511559919..560929 [-], 101154293462hypothetical protein 
33lpl0512561079..561813 [-], 73554293463hypothetical protein 
34lpl0513 (dotV)561897..562439 [+], 54354293464hypothetical protein  IcmC
35lpl0514562582..562878 [-], 29754293465hypothetical protein 
36lpl0515 (pssA)563096..563833 [+], 73854293466hypothetical protein 
37lpl0516564080..564349 [-], 27054293467hypothetical protein 
38lpl0517564540..564839 [-], 30054293468hypothetical protein 
39lpl0518 (rpoN)564866..566260 [-], 139554293469RNA polymerase factor sigma-54 
40lpl0519 (rpmG)566470..566634 [-], 1655429347050S ribosomal protein L33 
41lpl0520 (rpmB)566649..566885 [-], 2375429347150S ribosomal protein L28 
 
Region 2: 617992..618618
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1lpl0567613744..615438 [+], 169554293517hypothetical protein 
2lpl0569616397..616690 [+], 29454293518hypothetical protein 
3lpl0570616687..617571 [+], 88554293519hypothetical protein 
4lpl0571 (lvgA)617992..618618 [+], 62754293520hypothetical protein  LvgAaccesspry protein
5lpl0572618648..619025 [-], 37854293521hypothetical protein 
6lpl0573619175..620314 [-], 114054293522hypothetical protein 
7lpl0574 (sdhC)620635..621009 [+], 37554293523succinate dehydrogenase, cytochrome b556 subunit 
8lpl0575 (sdhD)621003..621350 [+], 34854293524succinate dehydrogenase, hydrophobic membrane anchor protein 
9lpl0576 (sdhA)621352..623121 [+], 177054293525succinate dehydrogenase flavoprotein subunit 
 
Region 3: 2968136..2986785
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1lpl25972963763..2964830 [-], 106854295510hypothetical protein 
2lpl25982964893..2966218 [+], 132654295511hypothetical protein 
3lpl25992966215..2967519 [+], 130554295512hypothetical protein 
4lpl26002967516..2968061 [+], 54654295513hypothetical protein 
5lpl2601 (dotD)2968136..2968627 [+], 49254295514lipoprotein DotD  DotD
6lpl2602 (dotC)2968608..2969519 [+], 91254295515defect in organelle trafficking lipoprotein DotC  DotC
7lpl2603 (dotB)2969519..2970652 [+], 113454295516defect in organelle trafficking protein DotB (ATPase)  DotB
8lpl26042970737..2972461 [+], 172554295517hypothetical protein 
9lpl26052972503..2973300 [-], 79854295518hypothetical protein 
10lpl26062973330..2974274 [-], 94554295519hypothetical protein 
11lpl26072974511..2975533 [+], 102354295520hypothetical protein 
12lpl26082975807..2976694 [+], 88854295521hypothetical protein 
13lpl26092976833..2977540 [+], 70854295522hypothetical protein 
14lpl2610 (dlpA)2977527..2979374 [+], 184854295523DlpA protein (isocitrate and isopropylmalate dehydrogenase family protein) 
15lpl26112979374..2980234 [+], 86154295524hypothetical protein 
16lpl2612 (sbpA)2980363..2981079 [-], 71754295525hypothetical protein 
17lpl2613 (dotA)2981210..2984353 [-], 314454295526defect in organelle trafficking protein DotA  DotA
18lpl2614 (icmV)2984350..2984805 [-], 45654295527intracellular multiplication protein IcmV  IcmV
19lpl2615 (icmW)2984918..2985373 [+], 45654295528intracellular multiplication protein IcmW  IcmWaccesspry protein
20lpl2616 (icmX)2985373..2986785 [+], 141354295529intracellular multiplication protein IcmX  IcmX
21lpl2617 (lphB)2986786..2988429 [-], 164454295530hypothetical protein 
22lpl26182988796..2991339 [+], 254454295531hypothetical protein 
 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes

Effectors
RavA, CegC1, lpl0022, RavB, AnkQ/legA10, lpl0044, lpl0045, Ceg2, Lem1, Ceg4, VipF, CegC2, lpl0130, SdhB, RavD, RavC, LegU1, lpl0244, RavE, RavF, RavG, Ceg7, SidE/laiD, Ceg8, Ceg9, lpl0310, lpl0313, SdbA, LegG2, Ceg10, Lem2, lpl0347, lpl0405, lpl0406, lpl0418, SdhA, VipA, LegA7/ceg11, AnkG/ankZ/legA7, lpl0447, LegY, AnkJ/legA11, Ceg14, Ceg15, AnkC/legA12, LegD2, lpl0557, SidA, lpl0671, WipB, AnkN/ankX legA8, Lem3, lpl0753, RavH, Ceg18, RavI, LidA, lpl0992, SidK, RavK, Lem4, lpl1105, RavL, Lem5, RavN, Ceg19, lpl1129, lpl1144, CegC3, Lem7, lpl1153, lpl1154, RavP, RavQ, lpl1165, RavR, lpl1179, RavS, VpdB, lpl1236, LegT, VpdC, SidB, lpl1387, Lem23, Ceg23, LegL2, Lem11, LegC6, RavY, Lem10, RavX, Lgt3/legc5, lpl1544, LegK1, LegL1, lpl1591, LegL3, lpl1626, lpl1631, lpl1632, lpl1635, MavA, lpl1652, PpeA/legC3, PpeB, lpl1675, AnkI/legAS4, lpl1715, lpl1716, lpl1740, MarB, lpl1766, Ceg25, Lem14, YlfB/legC2, lpl1850, LegLC8, lpl1871, lpl1888, Lem15, Lem17, RalF, LegC4, LirA, LirB, PieE, PieF, PieG/legG1, SetA, lpl1961, lpl2028, LegK2, AnkB/legAU13/ceg27, MavC, lpl2076, lpl2077, SdeC, SdeC, SidJ, SdeB, LaiA/SdeA, lpl2088, Lem19, LegS2, CegC4, CegC4, WipC, LegA2, Lem20, LpnE, lpl2149, Lem21, lpl2197, YlfA/legC7, AnkH/legA3, ankW, Ceg28, AnkK/legA5, lpl2247, Lem22, MavE, MavF, lpl2281, lpl2300, SdbC, LegL6, LegL6, Lem23, Ceg29, VpdA, Lem24, LegA1, lpl2343, Lem25, MavG, MavH, Ceg30, lpl2355, lpl2363, MavI, AnkF/legA14/ceg31, AnkD/legA15, lpl2380, SidM/drrA, SidD, SdbB, LepB, MavJ, SidI/ceg32, lpl2427, SdjA, SdeD, SdcA, SidC, MavL, LnaB, Lem27, lpl2459, lpl2460, lpl2462, lpl2473, lpl2480, LegK3, MavM, SidF, LegS1, Ceg33, Lem28, lpl2553, lpl2562, MavV, lpl2619, LegD1, WipA, LegN, lpl2669, lpl2670, LepA, Lem29, VipE, MavN, Ceg34, lpl2743, lpl2744, lpl2756, lpl2787, lpl2792, lpl2797, lpl2798, lpl2801, lpl2830, lpl2865, lpl2904, LegP, lpl2928

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1lpl0008 11140..12399 [-], 126054292972hypothetical protein  RavA
2lpl0012 14858..16435 [-], 157854292976hypothetical protein  CegC1
3lpl0022 27647..28126 [+], 48054292986hypothetical protein  lpl0022
4lpl0031 38608..39498 [-], 89154292995hypothetical protein  RavB
5lpl0038 45262..46764 [-], 150354293002hypothetical protein  AnkQ/legA10
6lpl0044 50805..51017 [-], 21354293008hypothetical protein  lpl0044
7lpl0045 51227..51625 [+], 39954293009hypothetical protein  lpl0045
8lpl0061 66159..67250 [+], 109254293025hypothetical protein  Ceg2
9lpl0089 102895..106869 [+], 397554293052hypothetical protein  Lem1
10lpl0096 113342..114535 [+], 119454293059hypothetical protein  Ceg4
11lpl0103 121779..122639 [-], 86154293066hypothetical protein  VipF
12lpl0125 145792..149100 [+], 330954293087hypothetical protein  CegC2
13lpl0130 153965..156433 [+], 246954293092hypothetical protein  lpl0130
14lpl0135 (sdhB)159856..165483 [-], 562854293097SdhB protein, substrate of the Dot/Icm system  SdhB
15lpl0224 251051..252028 [+], 97854293178hypothetical protein  RavD
16lpl0233 258884..259639 [-], 75654293187hypothetical proteinexperimental  RavC
17lpl0234 259905..260471 [-], 56754293188hypothetical protein  LegU1
18lpl0244 270792..271703 [+], 91254293197hypothetical protein  lpl0244
19lpl0251 279832..280836 [-], 100554293203hypothetical protein  RavE
20lpl0252 281038..282291 [-], 125454293204hypothetical protein  RavF
21lpl0264 296730..297317 [+], 58854293216hypothetical protein  RavG
22lpl0281 313793..314899 [+], 110754293233hypothetical protein  Ceg7
23lpl0288 (sidE)322701..327188 [-], 448854293240SidE protein, substrate of the Dot/Icm system  SidE/laiD
24lpl0294 334143..334910 [+], 76854293246hypothetical protein  Ceg8
25lpl0300 343337..344062 [+], 72654293252hypothetical protein  Ceg9
26lpl0310 353217..354209 [+], 99354293262hypothetical protein  lpl0310
27lpl0313 358317..358715 [+], 39954293265hypothetical protein  lpl0313
28lpl0327 (sdbA)372667..376020 [+], 335454293279SdbA protein, substrate of the Dot/Icm system  SdbA
29lpl0328 376263..377702 [+], 144054293280hypothetical protein  LegG2
30lpl0336 387343..388467 [-], 112554293288hypothetical protein  Ceg10
31lpl0337 388704..389387 [+], 68454293289hypothetical protein  Lem2
32lpl0347 399923..400615 [+], 69354293299hypothetical protein  lpl0347
33lpl0405 453134..453439 [-], 30654293356hypothetical protein  lpl0405
34lpl0406 453613..456303 [-], 269154293357hypothetical protein  lpl0406
35lpl0418 463132..463731 [+], 60054293369hypothetical protein  lpl0418
36lpl0419 (sdhA)463771..468060 [-], 429054293370SdhA, substrate of the Dot/Icm system  SdhA
37lpl0433 480943..481962 [-], 102054293384hypothetical protein  VipA
38lpl0444 489199..489861 [-], 66354293395hypothetical protein  LegA7/ceg11
39lpl0445 490134..491642 [+], 150954293396hypothetical protein  AnkG/ankZ/legA7
40lpl0447 493372..493962 [+], 59154293398hypothetical protein  lpl0447
41lpl0465 509327..510634 [-], 130854293416hypothetical protein  LegY
42lpl0479 520626..521426 [-], 80154293430hypothetical protein  AnkJ/legA11
43lpl0480 521696..523705 [+], 201054293431hypothetical protein  Ceg14
44lpl0481 524521..525459 [-], 93954293432hypothetical protein  Ceg15
45lpl0523 569045..570532 [+], 148854293474hypothetical proteinexperimental  AnkC/legA12
46lpl0554 602673..603605 [+], 93354293505hypothetical protein  LegD2
47lpl0557 605582..606430 [-], 84954293508hypothetical protein  lpl0557
48lpl0658 (sidA)709244..710668 [-], 142554293605SidA protein, subsrate of the Dot/Icm transport system  SidA
49lpl0671 725691..727028 [-], 133854293618hypothetical protein  lpl0671
50lpl0679 735605..737179 [+], 157554293626hypothetical protein  WipB
51lpl0732 828401..831238 [+], 283854293679hypothetical protein  AnkN/ankX legA8
52lpl0733 831245..832903 [-], 165954293680hypothetical protein  Lem3
53lpl0753 854531..855526 [+], 99654293700hypothetical protein  lpl0753
54lpl0770 872965..874389 [+], 142554293717hypothetical protein  RavH
55lpl0929 1043014..1043718 [-], 70554293873hypothetical protein  Ceg18
56lpl0957 1071042..1072049 [-], 100854293901hypothetical protein  RavI
57lpl0971 (lidA)1086944..1089133 [-], 219054293915LidA protein, substrate of the Dot/Icm system  LidA
58lpl0992 1121580..1122821 [-], 124254293936hypothetical protein  lpl0992
59lpl0997 1128576..1130270 [-], 169554293940hypothetical protein  SidK
60lpl0998 1130737..1131477 [+], 74154293941hypothetical protein  RavK
61lpl1100 1224090..1225058 [+], 96954294038hypothetical protein  Lem4
62lpl1105 1229553..1230815 [-], 126354294042hypothetical protein  lpl1105
63lpl1108 1233244..1234125 [-], 88254294045hypothetical protein  RavL
64lpl1114 1238256..1238936 [+], 68154294051hypothetical protein  Lem5
65lpl1115 1239045..1239683 [-], 63954294052hypothetical protein  RavN
66lpl1126 1252026..1252760 [+], 73554294062hypothetical protein  Ceg19
67lpl1129 1255813..1256403 [-], 59154294065hypothetical protein  lpl1129
68lpl1144 1273787..1274740 [-], 95454294080hypothetical protein  lpl1144
69lpl1150 1280208..1280681 [-], 47454294086hypothetical protein  CegC3
70lpl1151 1280975..1283284 [+], 231054294087hypothetical protein  Lem7
71lpl1153 1285000..1285440 [+], 44154294089hypothetical protein  lpl1153
72lpl1154 1285760..1287244 [+], 148554294090hypothetical protein  lpl1154
73lpl1159 1292757..1293581 [-], 82554294095hypothetical protein  RavP
74lpl1161 1295580..1296662 [+], 108354294097hypothetical protein  RavQ
75lpl1165 1301149..1301286 [+], 13854294101hypothetical protein  lpl1165
76lpl1174 1308670..1310658 [-], 198954294110hypothetical protein  RavR
77lpl1179 1316558..1316977 [+], 42054294115hypothetical protein  lpl1179
78lpl1192 1331242..1333101 [+], 186054294128hypothetical protein  RavS
79lpl1235 1375976..1377715 [+], 174054294171hypothetical protein  VpdB
80lpl1236 1378253..1379317 [+], 106554294172hypothetical protein  lpl1236
81lpl1282 1430303..1432699 [-], 239754294217hypothetical protein  LegT
82lpl1377 1536614..1539265 [+], 265254294312hypothetical protein  VpdC
83lpl1384 (sidB)1544945..1546198 [+], 125454294319substrate of the Dot/Icm system  SidB
84lpl1387 1549101..1550360 [+], 126054294322hypothetical protein  lpl1387
85lpl1398 1560527..1560922 [+], 39654294330hypothetical protein  Lem23
86lpl1402 1564214..1565533 [+], 132054294334hypothetical protein  Ceg23
87lpl1423 1589971..1590819 [+], 84954294355hypothetical protein  LegL2
88lpl1427 1593472..1594533 [+], 106254294358hypothetical protein  Lem11
89lpl1437 1602384..1604402 [+], 201954294368hypothetical protein  LegC6
90lpl1475 1644559..1645290 [+], 73254294406hypothetical protein  RavY
91lpl1530 1706246..1708054 [-], 180954294461hypothetical protein  Lem10
92lpl1539 1713583..1714614 [-], 103254294470hypothetical protein  RavX
93lpl1540 1714824..1717253 [-], 243054294471hypothetical protein  Lgt3/legc5
94lpl1544 1719433..1720182 [+], 75054294475hypothetical protein  lpl1544
95lpl1545 1720236..1721825 [-], 159054294476hypothetical proteinexperimental  LegK1interaction
96lpl1579 1758826..1760220 [+], 139554294508hypothetical protein  LegL1
97lpl1591 1767527..1768045 [-], 51954294515hypothetical protein  lpl1591
98lpl1625 1800655..1802136 [+], 148254294549hypothetical protein  LegL3
99lpl1626 1802230..1803348 [-], 111954294550hypothetical protein  lpl1626
100lpl1631 1813235..1814638 [-], 140454294555hypothetical protein  lpl1631
101lpl1632 1814799..1816166 [-], 136854294556hypothetical protein  lpl1632
102lpl1635 1820599..1821489 [+], 89154294559hypothetical protein  lpl1635
103lpl1650 1837444..1838646 [-], 120354294574hypothetical protein  MavA
104lpl1652 1840130..1840756 [+], 62754294576hypothetical protein  lpl1652
105lpl1660 1852250..1853929 [+], 168054294584hypothetical protein  PpeA/legC3
106lpl1661 1854065..1855705 [+], 164154294585hypothetical protein  PpeB
107lpl1675 1868663..1869109 [+], 44754294599hypothetical protein  lpl1675
108lpl1682 1874751..1876436 [-], 168654294606hypothetical protein  AnkI/legAS4
109lpl1715 1913792..1915105 [-], 131454294638hypothetical protein  lpl1715
110lpl1716 1915118..1915726 [-], 60954294639hypothetical protein  lpl1716
111lpl1740 1945150..1945632 [+], 48354294663hypothetical protein  lpl1740
112lpl1761 1963939..1965135 [-], 119754294684hypothetical protein  MarB
113lpl1766 1970055..1970990 [-], 93654294689hypothetical protein  lpl1766
114lpl1800 2014570..2015994 [+], 142554294723hypothetical protein  Ceg25
115lpl1817 2031015..2031677 [-], 66354294740hypothetical protein  Lem14
116lpl1845 2066116..2067333 [+], 121854294768hypothetical protein  YlfB/legC2
117lpl1850 2070292..2071623 [-], 133254294773hypothetical protein  lpl1850
118lpl1852 2072881..2074542 [-], 166254294775hypothetical protein  LegLC8
119lpl1871 2093085..2094890 [-], 180654294794hypothetical protein  lpl1871
120lpl1888 2113219..2116014 [+], 279654294811hypothetical protein  lpl1888
121lpl1903 2133646..2134260 [-], 61554294825hypothetical protein  Lem15
122lpl1918 2152751..2154085 [-], 133554294839hypothetical protein  Lem17
123lpl1919 (ralF)2154317..2155513 [+], 119754294840RalF protein, translocated into host cells by the Dot/Icm system  RalF
124lpl1922 2157659..2159893 [-], 223554294843hypothetical protein  LegC4
125lpl1934 2172470..2172721 [+], 25254294855hypothetical protein  LirA
126lpl1936 2174332..2174898 [+], 56754294857hypothetical protein  LirB
127lpl1941 2177683..2179476 [-], 179454294862hypothetical protein  PieE
128lpl1950 2187964..2188341 [-], 37854294871hypothetical protein  PieF
129lpl1953 2191194..2192636 [+], 144354294874hypothetical protein  PieG/legG1
130lpl1955 2193484..2195379 [-], 189654294876hypothetical protein  SetA
131lpl1961 2202418..2205234 [-], 281754294882hypothetical protein  lpl1961
132lpl2028 2271094..2272137 [-], 104454294949hypothetical protein  lpl2028
133lpl2066 2327815..2329452 [-], 163854294987hypothetical proteinexperimental  LegK2interaction
134lpl2072 2334238..2334756 [+], 51954294993hypothetical protein  AnkB/legAU13/ceg27
135lpl2075 2338045..2339493 [+], 144954294996hypothetical protein  MavC
136lpl2076 2339590..2340849 [+], 126054294997hypothetical protein  lpl2076
137lpl2077 2340937..2341296 [+], 36054294998hypothetical protein  lpl2077
138lpl2081 (sdeC)2345151..2349755 [+], 460554295001SdeC protein, substrate of the Dot/Icm system  SdeC
139lpl2082 2349970..2351091 [+], 112254295002hypothetical protein  SdeC
140lpl2083 2351144..2353768 [+], 262554295003hypothetical protein  SidJ
141lpl2084 (sdeB)2354046..2359808 [+], 576354295004hypothetical protein  SdeB
142lpl2085 (sdeA)2360178..2364809 [+], 463254295005hypothetical protein  LaiA/SdeA
143lpl2088 2367287..2368654 [-], 136854295008hypothetical protein  lpl2088
144lpl2093 2373881..2375140 [+], 126054295013hypothetical protein  Lem19
145lpl2102 2381661..2383478 [-], 181854295022hypothetical protein  LegS2
146lpl2123 2425425..2426006 [-], 58254295043hypothetical protein  CegC4
147lpl2124 2426366..2426902 [-], 53754295044hypothetical protein  CegC4
148lpl2131 2433338..2434438 [+], 110154295051hypothetical protein  WipC
149lpl2140 2442928..2444523 [-], 159654295060hypothetical protein  LegA2
150lpl2141 2444544..2446316 [-], 177354295061hypothetical protein  Lem20
151lpl2147 2452798..2453919 [-], 112254295067hypothetical protein  LpnE
152lpl2149 2455342..2455830 [-], 48954295069hypothetical protein  lpl2149
153lpl2174 2486875..2489109 [-], 223554295094hypothetical protein  Lem21
154lpl2197 2512115..2512765 [+], 65154295117hypothetical protein  lpl2197
155lpl2217 2531977..2533254 [-], 127854295137hypothetical protein  YlfA/legC7
156lpl2219 2536840..2538243 [-], 140454295139hypothetical proteinexperimental  AnkH/legA3, ankW
157lpl2230 2546271..2549750 [-], 348054295150hypothetical protein  Ceg28
158lpl2242 2559369..2561294 [-], 192654295162hypothetical proteinexperimental  AnkK/legA5
159lpl2247 2567227..2568105 [+], 87954295167hypothetical protein  lpl2247
160lpl2248 2568369..2568752 [+], 38454295168hypothetical protein  Lem22
161lpl2265 2582569..2583084 [+], 51654295185hypothetical protein  MavE
162lpl2273 2591001..2591852 [+], 85254295193hypothetical proteinexperimental  MavF
163lpl2281 (lporfX)2599403..2599846 [+], 44454295201hypothetical protein  lpl2281
164lpl2300 2617353..2618807 [+], 145554295217hypothetical protein  lpl2300
165lpl2315 (sdbC)2638570..2639874 [-], 130554295232SdbC proteins, substrate of the Dot/Icm system  SdbC
166lpl2316 2640142..2641026 [+], 88554295233hypothetical protein  LegL6
167lpl2323 2647610..2648674 [+], 106554295240hypothetical protein  LegL6
168lpl2329 (lidE)2654734..2655831 [+], 109854295246hypothetical protein  Lem23
169lpl2332 2658334..2659152 [+], 81954295249hypothetical protein  Ceg29
170lpl2334 2661327..2663324 [+], 199854295251hypothetical protein  VpdA
171lpl2335 2663427..2664254 [-], 82854295252hypothetical protein  Lem24
172lpl2339 2667665..2668771 [+], 110754295256hypothetical protein  LegA1
173lpl2343 2672575..2673126 [+], 55254295260hypothetical protein  lpl2343
174lpl2345 2675381..2677642 [-], 226254295262hypothetical protein  Lem25
175lpl2347 2679751..2681094 [-], 134454295264hypothetical protein  MavG
176lpl2348 2681582..2682382 [-], 80154295265hypothetical protein  MavH
177lpl2353 2684351..2686129 [+], 177954295268hypothetical protein  Ceg30
178lpl2355 2687571..2688056 [+], 48654295270hypothetical protein  lpl2355
179lpl2363 2693790..2694329 [-], 54054295278hypothetical protein  lpl2363
180lpl2364 2694574..2695125 [-], 55254295279hypothetical protein  MavI
181lpl2370 2699064..2701829 [-], 276654295285hypothetical protein  AnkF/legA14/ceg31
182lpl2375 2704939..2706354 [+], 141654295290hypothetical protein  AnkD/legA15
183lpl2380 2709431..2710048 [+], 61854295295hypothetical protein  lpl2380
184lpl2384 2713562..2715481 [-], 192054295299hypothetical protein  SidM/drrA
185lpl2385 2715575..2717098 [+], 152454295300hypothetical protein  SidD
186lpl2402 (sdbB)2731734..2733080 [+], 134754295317SdbB protein ( substrate of the Dot/Icm system)  SdbB
187lpl2411 (lepB)2740414..2744298 [+], 388554295326effector protein B, substrate of the Dot/Icm secretion system  LepB
188lpl2420 2751562..2752470 [+], 90954295335hypothetical protein  MavJ
189lpl2426 2757400..2760228 [+], 282954295341hypothetical protein  SidI/ceg32
190lpl2427 2760248..2761117 [+], 87054295342hypothetical protein  lpl2427
191lpl2430 2763914..2766337 [-], 242454295345hypothetical protein  SdjA
192lpl2431 (sdeD)2766408..2767556 [-], 114954295346SdeD protein (substrate of the Dot/Icm system)  SdeD
193lpl2432 (sdcA)2767861..2770578 [+], 271854295347SdcA protein, paralog of SidC (substrate of the Dot/Icm system)  SdcA
194lpl2433 (sidC)2770729..2773455 [+], 272754295348SidC protein (substrate of the Dot/Icm system)  SidC
195lpl2446 2786365..2787609 [-], 124554295361hypothetical protein  MavL
196lpl2447 2788074..2789693 [-], 162054295362hypothetical protein  LnaB
197lpl2449 2791622..2793304 [-], 168354295364hypothetical protein  Lem27
198lpl2459 2800612..2801958 [-], 134754295374hypothetical protein  lpl2459
199lpl2460 2802610..2803017 [-], 40854295375hypothetical protein  lpl2460
200lpl2462 2803728..2804555 [-], 82854295377hypothetical protein  lpl2462
201lpl2473 2812749..2814416 [-], 166854295388hypothetical protein  lpl2473
202lpl2480 2820305..2821147 [-], 84354295394hypothetical protein  lpl2480
203lpl2481 2821747..2823135 [+], 138954295395hypothetical proteinexperimental  LegK3
204lpl2499 2850379..2851110 [-], 73254295413hypothetical protein  MavM
205lpl2507 (sidF)2857821..2860559 [+], 273954295421substrate of the Dot/Icm system  SidF
206lpl2511 2864400..2865557 [-], 115854295425hypothetical proteinexperimental  LegS1
207lpl2514 2869002..2869505 [-], 50454295428hypothetical proteinexperimental  Ceg33
208lpl2526 2881440..2882681 [+], 124254295440hypothetical protein  Lem28
209lpl2553 2916347..2917099 [-], 75354295467hypothetical protein  lpl2553
210lpl2562 2925427..2926638 [-], 121254295476hypothetical protein  lpl2562
211lpl2563 2926990..2928372 [-], 138354295477hypothetical protein  MavV
212lpl2619 2991590..2992120 [+], 53154295532hypothetical protein  lpl2619
213lpl2621 2993116..2993946 [-], 83154295534hypothetical protein  LegD1
214lpl2646 3016605..3018167 [-], 156354295559hypothetical protein  WipA
215lpl2648 3019578..3020609 [+], 103254295561hypothetical protein  LegN
216lpl2669 3039714..3040883 [-], 117054295582hypothetical protein  lpl2669
217lpl2670 3041044..3042963 [-], 192054295583hypothetical protein  lpl2670
218lpl2708 (lepA)3088528..3091887 [+], 336054295621effector protein A, substrate of the Dot/Icm secretion system  LepA
219lpl2719 3103748..3105061 [-], 131454295632hypothetical protein  Lem29
220lpl2728 3118685..3119545 [+], 86154295641hypothetical protein  VipE
221lpl2730 3120944..3122995 [+], 205254295643hypothetical proteinexperimental  MavN
222lpl2741 3134300..3136033 [-], 173454295653hypothetical protein  Ceg34
223lpl2743 3137505..3138761 [+], 125754295655hypothetical protein  lpl2743
224lpl2744 3139140..3140780 [-], 164154295656hypothetical protein  lpl2744
225lpl2756 3153814..3155001 [-], 118854295668hypothetical protein  lpl2756
226lpl2787 3185408..3186292 [-], 88554295699hypothetical protein  lpl2787
227lpl2792 3191843..3193576 [-], 173454295704hypothetical protein  lpl2792
228lpl2797 3197993..3198727 [-], 73554295709hypothetical protein  lpl2797
229lpl2798 3199037..3199579 [-], 54354295710hypothetical protein  lpl2798
230lpl2801 3202073..3203986 [+], 191454295713hypothetical protein  lpl2801
231lpl2830 3229721..3231208 [+], 148854295741hypothetical protein  lpl2830
232lpl2865 3269711..3270445 [-], 7355429577516S ribosomal RNA methyltransferase RsmE  lpl2865
233lpl2904 3311669..3314284 [-], 261654295814hypothetical protein  lpl2904
234lpl2927 3338486..3339286 [+], 80154295836hypothetical protein  LegP
235lpl2928 3339283..3341121 [-], 183954295837hypothetical protein  lpl2928

interaction This effector contains information of interaction.

Download FASTA format files
Proteins        Genes
The information on structure of this T4ss

#NameImageResourceDetailReference
1AMP-PNP•LegK45CKW PDB (5CKW)Crystal structure of LegK4_AMPPNP Kinase.(1) PubMed: 26419332
2Apo-LegK45CLR PDB (5CLR)Crystal structure of LegK4_APO Kinase.(2) PubMed: 26419332
(1) Flayhan A et al. (2015). The structure of Legionella pneumophila LegK4 type four secretion system (T4SS) effector reveals a novel dimeric eukaryotic-like kinase. Sci Rep. 5:14602. [PudMed:26419332] experimental
(2) Flayhan A et al. (2015). The structure of Legionella pneumophila LegK4 type four secretion system (T4SS) effector reveals a novel dimeric eukaryotic-like kinase. Sci Rep. 5:14602. [PudMed:26419332] experimental
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1IcmS (54293435)C-terminal fragment-The IcmS adaptors essential for translocation of different subsets of effectors through the Legionella Dot/Icm translocation apparatus.(1) PubMed: 28714967
2IcmS/IcmW (chaperones)CT Val-2 and Leu-3 required for translocationAnkBThe IcmSW chaperone complex is essential for translocation of AnkB.(2) PubMed: 18811729
3IcmS/IcmW (chaperones)C-terminal hydrophbic tail, CT20aasidGThe IcmSW complex interacts with a central region of the SidG protein. It resulted in a conformational change in the SidG protein as determined by differences in protease sensitivity in vitro. (3) PubMed: 18069892
4IcmS/IcmW (chaperones)C-terminal hydrophobic tail, CT100aa sufficientsidCIcmSW could enhance effector protein translocation by mediating a conformational change. (4) PubMed: 16714592
5IcmS/IcmW (chaperones)C-terminal hydrophobic tail. CT30aa sufficientlegS2LegS2 translocation depends on a functional IcmS IcmW complex.(5) PubMed: 19438520
6IcmW (54295528)C-terminal fragment-The IcmW adaptors essential for translocation of different subsets of effectors through the Legionella Dot/Icm translocation apparatus.(6) PubMed: 19946141
7LvgA (54293520)C-terminal fragment-The LvgA adaptors essential for translocation of different subsets of effectors through the Legionella Dot/Icm translocation apparatus.(7) PubMed: 28714967
Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Kwak MJ et al. (2017). Architecture of the type IV coupling protein complex of Legionella pneumophila. Nat Microbiol. 2:17114. [PudMed:28714967] experimental
(2) Al-Khodor S; Price CT; Habyarimana F; Kalia A; Abu Kwaik Y (2008). A Dot/Icm-translocated ankyrin protein of Legionella pneumophila is required for intracellular proliferation within human macrophages and protozoa. Mol Microbiol. 70(4):908-23. [PudMed:18811729] experimental
(3) Cambronne ED; Roy CR (2007). The Legionella pneumophila IcmSW complex interacts with multiple Dot/Icm effectors to facilitate type IV translocation. PLoS Pathog. 3(12):e188. [PudMed:18069892] experimental
(4) VanRheenen SM; Luo ZQ; O'Connor T; Isberg RR (2006). Members of a Legionella pneumophila family of proteins with ExoU (phospholipase A) active sites are translocated to target cells. Infect Immun. 74(6):3597-606. [PudMed:16714592] experimental
(5) Degtyar E; Zusman T; Ehrlich M; Segal G (2009). A Legionella effector acquired from protozoa is involved in sphingolipids metabolism and is targeted to the host cell mitochondria. Cell Microbiol. 11(8):1219-35. [PudMed:19438520] experimental in_silico
(6) Alvarez-Martinez CE; Christie PJ (2009). Biological diversity of prokaryotic type IV secretion systems. Microbiol Mol Biol Rev. 73(4):775-808. [PudMed:19946141]
(7) Kwak MJ et al. (2017). Architecture of the type IV coupling protein complex of Legionella pneumophila. Nat Microbiol. 2:17114. [PudMed:28714967] experimental
The information on host-pathogen interaction.

#Name(Protein GI)Host site/SubstrateSourceFunctionReference
1LegK1 (54294476)NF-κBmurineLegK1 can imitate host IKK and phosphorylates the Ser32 and Ser36 residues of the inhibitory IkBa protein directly. This process leads to IkBa ubiquitination and degradation, which results in subsequent nuclear translocation of p65.(1) PubMed: 19666608insolico
2LegK2 (54294987)Arp2/3 complexhumanLegK2 phosphorylates the ARPC1B and ARP3 subunits of the ARP2/3 complex. This process inhibits actin nucleation on phagosomes, which results in the escape from host defenses of bacteria through controlling vesicle trafficking.(2) PubMed: 25944859experimental
experimental This T4SE is mentioned in the literature.
insolico This T4SE is highly identical with the protein mentioned in the literature.
(1) Ge J; Xu H; Li T; Zhou Y; Zhang Z; Li S; Liu L; Shao F (2009). A Legionella type IV effector activates the NF-kappaB pathway by phosphorylating the IkappaB family of inhibitors. Proc Natl Acad Sci U S A. 106(33):13725-30. [PudMed:19666608] experimental
(2) Michard C et al. (2015). The Legionella Kinase LegK2 Targets the ARP2/3 Complex To Inhibit Actin Nucleation on Phagosomes and Allow Bacterial Evasion of the Late Endocytic Pathway. MBio. 6(3):e00354-15. [PudMed:25944859] experimental
(1) Gomez-Valero L; Rusniok C; Cazalet C; Buchrieser C (2011). Comparative and functional genomics of legionella identified eukaryotic like proteins as key players in host-pathogen interactions. Front Microbiol. 2:208. [PudMed:22059087] in_silico
(2) Cazalet C; Rusniok C; Bruggemann H; Zidane N; Magnier A; Ma L; Tichit M; Jarraud S; Bouchier C; Vandenesch F; Kunst F; Etienne J; Glaser P; Buchrieser C (2004). Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity. Nat Genet. 36(11):1165-73. [PudMed:15467720]
 
in_silico This literature contains bioinformatics investigation

Genomics & Bioinformatics Group in MML, SJTU