| Effector Protein ID: 52842167 |
 T4SS name [ID] | Dot/Icm [5] |
| Gene symbol | sec7 |
| Locus tag | lpg1950 |
| Strain | Legionella pneumophila subsp. pneumophila str. Philadelphia 1 |
| Replicon | chromosome [GenBank: NC_002942] [Browse all T4SS(s) in this replicon] |
| Location [Strand] | 2178619..2179743 [+] |
| Product | guanine nucleotide exchange protein |
| Effector type | Protein |
| Descripton | RalF |
| T4CP requirement | T4CP Depedent |
| Target/Effection | RalF mediates Legionella-containing vacuole (LCV) recruitment of Arf1 (ADP-ribosylation factor 1), which is a GTPase responsible for mediating vesicular transport between Golgi and endoplastic reticulum. RalF colocalizes with Arf1 and activates it on the LCV after bacterial internalization |
| UniProt ID | Q5ZU58 |
| KEGG ID | lpn:lpg1950 |
| PDB ID | NA |
| Domain hit(s) vs Pfam | Sec7 [PF01369], Evalue: 1.7e-56, Aligned region: 9..192 |
| Protein Sequence: 374 a.a. [Download] |
| >gi|52842167|ref|YP_095966.1| guanine nucleotide exchange protein [Legionella pneumophila subsp. pneumophila str. Philadelphia 1] MHPEIEKAQREIIEAFNAKPKNGINKIKEICEQYKISPNEEIAEFFHQQRKNLDLEAVGDYLSSPEAENQ QVLKAFTSQMNFNGQSFVEGLRTFLKTFKLPGEAQKIDRLVQSFSGAYFQQNPDVVSNADAAYLLAFQTI MLNTDLHNPSIPEKNKMTVDGLKRNLRGGNNGGDFDAKFLEELYSEIKAKPFELNFVKTSPGYELTSTTL NKDSTFKKLDSFLHSTDVNINTVFPGIGDNVKTTVDQPKSWLSFFTGYKGTITLTDNKTSAQATIQVYTP NIFSKWLFGEQPRVIIQPGQTKESIDLAAKAAADFSSPVKNFKATYDYEVGDLIKAYDNQKKLITIERNL ALKEGVPKDPDAEMQKEKGRQLKF |
| - |
| Nucleotide Sequence: 1125 bp [Download] |
| >gi|52840256|ref|NC_002942.5|:2178619-2179743 Legionella pneumophila subsp. pneumophila str. Philadelphia 1 chromosome, complete genome ATGCATCCAGAAATTGAAAAAGCCCAAAGAGAGATTATTGAGGCATTCAATGCCAAGCCAAAAAATGGGA TAAATAAAATAAAAGAAATATGTGAACAATATAAAATTTCTCCCAATGAAGAGATAGCTGAGTTCTTTCA TCAACAAAGAAAAAATTTGGATTTAGAAGCGGTTGGCGATTATTTAAGCAGCCCAGAGGCAGAAAACCAG CAAGTATTAAAAGCTTTTACCAGTCAAATGAATTTCAATGGGCAAAGTTTTGTTGAGGGTTTAAGGACCT TTTTGAAAACATTTAAATTACCAGGCGAAGCTCAAAAAATTGACCGGTTAGTGCAAAGCTTTAGTGGTGC CTATTTCCAGCAAAATCCTGATGTGGTTTCCAATGCGGATGCTGCCTATCTTTTAGCATTTCAAACCATT ATGCTTAATACTGATTTACATAACCCAAGTATCCCTGAGAAAAATAAAATGACCGTGGATGGACTCAAAA GAAATCTCAGAGGAGGTAATAATGGTGGAGATTTTGATGCCAAATTTTTAGAGGAACTATACAGTGAGAT TAAAGCCAAACCTTTTGAGTTGAATTTCGTTAAAACCAGCCCAGGATATGAACTTACTTCAACCACTCTT AATAAAGATTCTACCTTTAAAAAGCTGGATTCATTCTTGCATTCAACGGATGTAAACATCAATACAGTTT TTCCAGGAATAGGTGATAATGTTAAAACAACTGTAGATCAACCAAAATCATGGTTAAGTTTTTTTACCGG ATATAAGGGAACAATAACGTTGACAGATAACAAAACATCCGCACAAGCTACTATCCAGGTTTATACTCCC AATATATTTTCAAAATGGCTATTTGGAGAACAACCGAGAGTAATAATACAACCTGGACAAACCAAAGAGT CAATCGATTTGGCAGCAAAAGCAGCAGCTGACTTTTCATCGCCAGTAAAAAATTTTAAAGCGACGTATGA TTATGAGGTAGGTGATTTAATAAAAGCATATGATAACCAGAAAAAACTCATTACGATTGAGAGAAACCTG GCACTTAAGGAGGGCGTTCCCAAAGATCCAGACGCTGAAATGCAAAAAGAAAAAGGTAGACAATTAAAAT TTTAA |
| Reference |
(1) Gomez-Valero L; Rusniok C; Cazalet C; Buchrieser C (2011). Comparative and functional genomics of legionella identified eukaryotic like proteins as key players in host-pathogen interactions. Front Microbiol. 2:208. [PudMed:22059087] 
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| (2) Luo ZQ (2012). Legionella secreted effectors and innate immune responses. Cell Microbiol. 14(1):19-27. [PudMed:21985602] |
(3) Burstein D; Zusman T; Degtyar E; Viner R; Segal G; Pupko T (2009). Genome-scale identification of Legionella pneumophila effectors using a machine learning approach. PLoS Pathog. 5(7):e1000508. [PudMed:19593377] 
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(4) Tiaden A; Spirig T; Carranza P; Bruggemann H; Riedel K; Eberl L; Buchrieser C; Hilbi H (2008). Synergistic contribution of the Legionella pneumophila lqs genes to pathogen-host interactions. J Bacteriol. 190(22):7532-47. [PudMed:18805977]
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(5) Pan X; Luhrmann A; Satoh A; Laskowski-Arce MA; Roy CR (2008). Ankyrin repeat proteins comprise a diverse family of bacterial type IV effectors. Science. 320(5883):1651-4. [PudMed:18566289]
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(6) Cambronne ED; Roy CR (2007). The Legionella pneumophila IcmSW complex interacts with multiple Dot/Icm effectors to facilitate type IV translocation. PLoS Pathog. 3(12):e188. [PudMed:18069892]
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(7) Shi C; Forsbach-Birk V; Marre R; McNealy TL (2006). The Legionella pneumophila global regulatory protein LetA affects DotA and Mip. Int J Med Microbiol. 296(1):15-24. [PudMed:16423685]
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| experimental literature |
| in silico analysis literature |