| Effector Protein ID: 52841713 |
 T4SS name [ID] | Dot/Icm [5] |
| Gene symbol | legK1 |
| Locus tag | lpg1483 |
| Strain | Legionella pneumophila subsp. pneumophila str. Philadelphia 1 |
| Replicon | chromosome [GenBank: NC_002942] [Browse all T4SS(s) in this replicon] |
| Location [Strand] | 1638274..1639863 [+] |
| Product | serine/threonine-protein kinase |
| Effector type | Protein |
| Descripton | LegK1 |
| T4CP requirement | T4CP Depedent |
| Target/Effection | The canonical serine/threonine kinase activity of LegK1 is essential for potent activation of the NF-kB pathway. LegK1 mimics host IKK and directly phosphorylates the Ser32 and Ser36 residues of the inhibitory IkBa protein, leading to IkBa ubiquitination/degradation and subsequent nuclear translocation of p65. (Ge and Shao, 2011) |
| UniProt ID | Q5ZVF7 |
| KEGG ID | lpn:lpg1483 |
| PDB ID | NA |
| Domain hit(s) vs Pfam | Pkinase [PF00069], Evalue: 7.3e-27, Aligned region: 86..362 Pkinase_Tyr [PF07714], Evalue: 2.4e-14, Aligned region: 86..290 |
| Protein Sequence: 529 a.a. [Download] |
| >gi|52841713|ref|YP_095512.1| serine/threonine-protein kinase [Legionella pneumophila subsp. pneumophila str. Philadelphia 1] MPRTMFFSVDAANLQNLQITNQKAFAALESYLNDNDSPGAWNNNQGYKYTHKDVTYCFSFNQSLVRRPRK SDQKKHTFEIFDPNKNPLGKGAYGIVYPILGTIQFEFGNAIKRPPKNKLVKIQNHSERDQSHSVLREYQG LLQSGHIAVKPPIFVENKESKFSYLIMEKAEGIVLEKFLNPIKRLDLSEEVPELNLINRIEITFAILKAI KEQVTDKHLIHRDIKPGNIIIDFSKSPPIAKVIDFGFVLRASEQDYRRCGTRAYRAPESFNTQALYTAKA DVWSTGRILSYLWGDKYTNYYISRDKGLDYVIEKSRNELLFSDPELELYLTKEDKSKIRSCLSAMLIVDP EERGSIDKAIKQFSQIDFEKYKRLKRSTNFEIDLTDNDIKLRKQLNSIHLHLITLQSKEKDLRNRACFDA ANAMSQLVSKLTAYTNYLEKNPDPFLIKRYKECCIAEIDLANLTLQNHRDALWLVAELSTAILLLGIGYL FAFGINYYNTGRLGLFSQTRSEKLVEEMKSSVLGIAVGN |
| - |
| Nucleotide Sequence: 1590 bp [Download] |
| >gi|52840256|ref|NC_002942.5|:1638274-1639863 Legionella pneumophila subsp. pneumophila str. Philadelphia 1 chromosome, complete genome ATGCCTCGTACCATGTTTTTTTCCGTTGATGCAGCTAATCTCCAAAATTTACAAATTACAAATCAAAAAG CTTTTGCAGCGTTGGAGAGTTATCTGAATGATAATGATTCACCAGGTGCCTGGAATAATAATCAAGGATA CAAATATACTCATAAGGATGTTACTTATTGCTTCTCATTTAATCAATCCTTAGTGAGACGCCCAAGAAAA TCGGATCAAAAAAAACACACATTTGAAATTTTTGACCCTAATAAGAACCCTTTAGGCAAGGGAGCGTACG GTATTGTGTATCCCATTTTAGGTACAATTCAGTTTGAATTTGGAAATGCAATTAAAAGACCACCAAAGAA TAAATTAGTTAAAATACAAAACCATAGTGAACGCGATCAAAGTCATTCTGTACTACGTGAATATCAAGGA CTTCTTCAATCAGGGCATATAGCGGTTAAACCGCCAATATTTGTAGAAAACAAAGAGAGTAAATTCAGTT ACTTGATTATGGAAAAAGCGGAGGGTATCGTTTTAGAGAAGTTTCTAAATCCCATTAAGCGGCTTGATTT GTCTGAAGAGGTACCTGAGCTTAATCTTATTAATCGGATTGAGATTACTTTTGCCATTTTAAAAGCAATT AAAGAGCAGGTGACGGATAAGCATTTGATTCATCGAGATATAAAACCAGGAAATATTATAATCGATTTTA GCAAGTCGCCTCCGATAGCTAAAGTAATCGATTTTGGTTTTGTGCTTAGGGCGTCAGAACAGGATTATCG TCGTTGTGGAACCCGAGCATACAGAGCGCCCGAATCTTTCAATACCCAGGCATTGTACACAGCAAAAGCT GATGTTTGGTCAACAGGCAGAATATTAAGTTATTTGTGGGGAGATAAATATACTAATTACTATATCTCTC GTGATAAGGGTCTGGACTACGTGATTGAGAAGTCAAGAAACGAGCTACTCTTCTCTGACCCGGAATTGGA ACTCTATTTAACCAAGGAAGATAAAAGTAAAATCAGATCGTGCCTAAGCGCAATGCTTATAGTTGACCCT GAGGAAAGAGGATCTATAGATAAGGCTATCAAGCAGTTTTCCCAAATTGATTTTGAAAAATATAAAAGAT TGAAACGCTCTACAAATTTTGAAATTGATTTAACAGATAATGATATTAAATTAAGAAAACAGCTCAATTC AATTCACCTGCATTTGATTACCCTACAGAGCAAAGAAAAAGATTTGCGTAATAGAGCATGTTTTGATGCA GCAAATGCAATGAGCCAATTGGTTAGTAAGCTCACCGCCTACACCAATTATCTTGAAAAAAATCCAGATC CATTTTTAATAAAACGTTATAAAGAGTGCTGCATAGCAGAAATAGATTTAGCCAATTTGACTTTGCAAAA TCACCGTGATGCCTTATGGTTAGTGGCTGAGTTATCAACTGCTATCCTTTTGCTTGGTATAGGGTATTTA TTTGCTTTTGGGATAAATTATTACAACACAGGTCGTCTGGGATTATTTTCTCAAACCAGGTCTGAGAAAT TGGTGGAGGAAATGAAAAGCTCTGTGTTGGGTATTGCGGTTGGAAATTAA |
| Reference |
| (1) Rolando M; Buchrieser C (2012). Post-translational modifications of host proteins by Legionella pneumophila: a sophisticated survival strategy. Future Microbiol. 7(3):369-81. [PudMed:22393890] |
(2) Gomez-Valero L; Rusniok C; Cazalet C; Buchrieser C (2011). Comparative and functional genomics of legionella identified eukaryotic like proteins as key players in host-pathogen interactions. Front Microbiol. 2:208. [PudMed:22059087] 
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| (3) Luo ZQ (2012). Legionella secreted effectors and innate immune responses. Cell Microbiol. 14(1):19-27. [PudMed:21985602] |
(4) Ge J; Xu H; Li T; Zhou Y; Zhang Z; Li S; Liu L; Shao F (2009). A Legionella type IV effector activates the NF-kappaB pathway by phosphorylating the IkappaB family of inhibitors. Proc Natl Acad Sci U S A. 106(33):13725-30. [PudMed:19666608] 
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| experimental literature |
| in silico analysis literature |