| Effector Protein ID: 289164734 |
 T4SS name [ID] | Dot/Icm [44] |
| Gene symbol | ralF |
| Locus tag | LLO_1397 |
| Strain | Legionella longbeachae NSW150 |
| Replicon | chromosome [GenBank: NC_013861] [Browse all T4SS(s) in this replicon] |
| Location [Strand] | 1661758..1662912 [-] |
| Product | RalF protein, translocated into host cells by the Dot/Icm system |
| Effector type | Protein |
| Descripton | RalF |
| T4CP requirement | T4CP Depedent |
| Target/Effection | RalF mediates Legionella-containing vacuole (LCV) recruitment of Arf1 (ADP-ribosylation factor 1), which is a GTPase responsible for mediating vesicular transport between Golgi and endoplastic reticulum. RalF colocalizes with Arf1 and activates it on the LCV after bacterial internalization |
| UniProt ID | D3HS78 |
| KEGG ID | llo:LLO_1397 |
| PDB ID | NA |
| Domain hit(s) vs Pfam | Sec7 [PF01369], Evalue: 6.3e-47, Aligned region: 10..193 |
| Protein Sequence: 384 a.a. [Download] |
| >gi|289164734|ref|YP_003454872.1| RalF protein, translocated into host cells by the Dot/Icm system [Legionella longbeachae NSW150] MINPELEKAQNEIITSFNSDPKKGIQLIKNICSTHGIDSAEQIADFFHRQKHKLDLDAVSDYLSKPDEES KKVLQNFTSQINFREQSFTEGFRVFLNTIKLPSEAQKIDRLVQSFGETYYQQNYENHIANKDAAYTLAYA VLILNTSLHNPNSRQKDKLTLSALKICLKGLNDGQDFEDAFLKKIYEEIKCKPFEFNLAKTTPGYLLTSS TLDDDLMFKKLDLLLQSSISTTEEIFPEIADNITVTFDKPKAWLKAFIGYEGTIKFTTESGRELANIQIY KPSLVSKWLFGAQPKVIIQPIDQDENPKEAIELAAKIAVLFESPVNSFKATYDYELSELISAYDQQHKEL VRKPFIPQFEKLRFFQRPCSEDNKEELMKSNGLK |
| - |
| Nucleotide Sequence: 1155 bp [Download] |
| >gi|289163353|ref|NC_013861.1|:c1662912-1661758 Legionella longbeachae NSW150 chromosome, complete genome ATGATTAATCCAGAATTAGAAAAAGCTCAGAATGAAATCATCACATCTTTTAATTCTGATCCTAAAAAAG GTATTCAACTAATAAAAAACATATGCAGTACCCATGGTATTGATTCAGCTGAACAAATTGCCGATTTCTT CCATAGACAAAAGCATAAATTGGATTTAGATGCAGTGAGTGATTATTTGAGTAAACCCGATGAGGAAAGC AAGAAAGTCTTACAAAATTTTACGAGCCAGATTAATTTTAGAGAACAAAGCTTTACAGAAGGTTTTAGAG TTTTTTTAAATACAATTAAATTACCTTCAGAAGCACAAAAAATTGATAGATTAGTTCAGAGTTTTGGCGA AACCTATTATCAGCAGAATTATGAGAATCATATTGCCAACAAAGATGCAGCCTATACTTTAGCCTATGCA GTTCTGATACTTAATACCAGTTTACATAATCCAAATTCGCGACAAAAAGATAAATTGACCCTAAGTGCTC TAAAAATATGTCTAAAAGGACTCAATGATGGCCAAGATTTTGAAGATGCTTTTTTAAAAAAAATATATGA AGAAATTAAATGTAAGCCTTTTGAATTTAATTTAGCTAAAACCACCCCTGGATATCTACTCACCTCCAGT ACTCTCGATGATGATCTTATGTTTAAAAAATTGGATTTATTGCTTCAGTCATCCATTAGCACTACTGAAG AAATTTTTCCAGAAATCGCTGATAATATTACCGTGACTTTTGATAAACCCAAAGCCTGGTTAAAAGCTTT CATTGGATATGAAGGTACAATAAAATTCACTACTGAATCAGGAAGGGAGTTAGCAAATATACAAATTTAT AAACCAAGTCTCGTTTCGAAATGGTTATTTGGGGCACAACCTAAAGTAATAATTCAACCCATTGATCAAG ATGAGAATCCTAAAGAAGCAATTGAATTAGCAGCAAAAATAGCAGTTCTTTTTGAATCACCTGTAAATAG TTTTAAAGCTACATATGATTATGAGTTAAGCGAATTAATTAGCGCTTATGATCAGCAACATAAAGAACTA GTCCGAAAACCATTTATTCCTCAATTCGAGAAGCTTAGATTCTTTCAAAGACCTTGTTCAGAAGACAATA AAGAAGAATTGATGAAATCTAATGGGTTGAAATAG |
| Reference |
(1) Gomez-Valero L; Rusniok C; Cazalet C; Buchrieser C (2011). Comparative and functional genomics of legionella identified eukaryotic like proteins as key players in host-pathogen interactions. Front Microbiol. 2:208. [PudMed:22059087] 
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| in silico analysis literature |