| Effector Protein ID: 190404482 |
 T4SS name [ID] | VirB [16] |
| Gene symbol | - |
| Locus tag | pRi2659_p139 |
| Strain | Agrobacterium rhizogenes plasmid pRi2659 (NC_010841) |
| Replicon | plasmid pRi2659 [GenBank: NC_010841] [Browse all T4SS(s) in this replicon] |
| Location [Strand] | 171300..172646 [+] |
| Product | type IV secretion system T-DNA border endonuclease VirD2 |
| Effector type | Protein |
| Descripton | VirD2 |
| T4CP requirement | T4CP Depedent |
| Target/Effection | VirD2 is an endonuclease that nicks the T-DNA region of Ti-/Ri-plasmids at border repeat sequences flanking T-DNA. During T-DNA processing, VirD2 covalently attaches to the 5' end of the resulting T-strand. VirD2 serves as a "pilot protein" to lead T-strands through the T4SS and into the host cell. Once in the host, VirD2 probably plays a major role in targeting T-strands to the nucleus |
| UniProt ID | A8W0J2 |
| KEGG ID | NA |
| PDB ID | NA |
| Domain hit(s) vs Pfam | Relaxase [PF03432], Evalue: 3.1e-32, Aligned region: 16..218 |
| Protein Sequence: 448 a.a. [Download] |
| >gi|190404482|ref|YP_001961113.1| rcorf138 [Agrobacterium rhizogenes] MPDRSQVIIRIVPGGGTKTLQQIINQLEYLSRKGKLELQRSARHLDTFVPPDEIRELALSWVQETGTYHE SRPDEERQQELTTHIIVSFPAGTSQVAAYAASREWAAEMFGSGAGGGRYNYLTAFHIDRDHPHLHVVVNR RELLGHGWLKISRRHPQLNYDALRITMAEISLRHGVVLEATSRAERGISERPMTFAQYRRLERQQANQIR FEDIDFEEFSPGGNDGEPDQSFNSSYGSLPQNVSENLRRNGGLQSVSEVRSRALIGSNGNGVTPDRVSSG NDSRSQSGSDKAFVDDGNLRNGPLTIAGDGQDLEGRSGMHRLATEPVTHTTSEDDVRQRPHRKRPRGDEE EQSGAKLTRVDGIRTGVTISAVPVAQDDPITSPIQPPGSNPLDDPGQTNIATDALPPTADRRQQGEPNSK RPRDDDAEPSIRKRSRDGRSQGDEGNRR |
| - |
| Nucleotide Sequence: 1347 bp [Download] |
| >gi|190404344|ref|NC_010841.1|:171300-172646 Agrobacterium rhizogenes plasmid pRi2659, complete sequence ATGCCCGATCGGTCTCAAGTCATCATCCGCATTGTGCCGGGAGGTGGGACGAAGACCCTCCAACAGATTA TCAACCAGTTGGAGTATCTATCTCGGAAGGGCAAGTTGGAGCTGCAGCGTTCGGCCCGACATCTCGATAC TTTCGTACCACCGGATGAAATCCGCGAACTCGCCCTAAGCTGGGTTCAAGAAACCGGGACTTATCACGAA AGTCGGCCAGACGAGGAAAGGCAACAGGAGTTGACGACCCACATTATTGTAAGCTTCCCTGCCGGTACAA GTCAGGTAGCAGCTTATGCAGCGAGCCGGGAGTGGGCAGCCGAGATGTTTGGGTCAGGCGCAGGGGGAGG CCGATACAACTATCTTACAGCCTTTCACATCGATCGCGATCACCCACATCTGCATGTGGTCGTTAATCGT CGCGAACTTTTGGGGCACGGCTGGCTGAAAATATCCCGGCGCCACCCCCAACTGAATTATGACGCCTTGC GCATTACAATGGCCGAGATTTCACTTCGTCATGGCGTCGTCCTCGAGGCGACGAGCCGAGCTGAGCGCGG CATCTCGGAGCGGCCGATGACTTTTGCCCAGTATCGACGCCTGGAGCGGCAGCAGGCCAATCAAATTCGT TTCGAGGATATTGATTTCGAAGAATTCTCGCCTGGGGGAAACGACGGAGAACCGGATCAATCTTTTAATT CTTCGTACGGTTCGCTGCCTCAAAACGTGTCAGAAAACTTGCGACGAAACGGCGGTCTGCAATCGGTGTC TGAAGTACGTTCCCGGGCACTGATCGGATCGAACGGCAACGGTGTCACGCCCGATCGTGTATCATCTGGA AACGATTCCCGTTCTCAATCCGGCAGCGATAAAGCCTTTGTGGACGATGGCAACTTGAGGAACGGCCCTC TCACCATCGCCGGAGACGGCCAGGATCTTGAAGGCCGTTCTGGCATGCATCGTTTGGCAACCGAACCCGT CACGCACACAACAAGCGAAGATGATGTTCGGCAACGGCCTCATCGAAAACGGCCTCGCGGTGATGAGGAA GAGCAGAGCGGCGCAAAACTGACCAGGGTAGACGGAATTCGGACGGGAGTGACAATCAGCGCCGTACCGG TTGCCCAGGACGATCCGATTACATCGCCGATCCAGCCCCCTGGATCAAATCCGTTGGATGACCCGGGGCA GACCAACATCGCTACGGACGCATTGCCTCCGACAGCCGATCGCCGGCAGCAGGGAGAGCCAAATTCTAAG CGTCCTCGTGATGATGACGCCGAGCCGAGCATTCGTAAGCGGTCAAGGGACGGGCGCAGCCAAGGGGATG AGGGAAACAGAAGGTAG |
| Reference |
(1) Hodges LD; Lee LY; McNett H; Gelvin SB; Ream W (2009). The Agrobacterium rhizogenes GALLS gene encodes two secreted proteins required for genetic transformation of plants. J Bacteriol. 191(1):355-64. [PudMed:18952790] 
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| experimental literature |