| Effector Protein ID: 190404372 |
 T4SS name [ID] | VirB [16] |
| Gene symbol | - |
| Locus tag | pRi2659_p029 |
| Strain | Agrobacterium rhizogenes plasmid pRi2659 (NC_010841) |
| Replicon | plasmid pRi2659 [GenBank: NC_010841] [Browse all T4SS(s) in this replicon] |
| Location [Strand] | 34384..39693 [-] |
| Product | rcorf28 |
| Effector type | Protein |
| Descripton | GALLS |
| T4CP requirement | T4CP Depedent |
| Target/Effection | GALLS can substitute for VirE2 in strains of Agrobacterium rhizogenes that lack virE2. GALLS can be made in two forms: GALLS-FL (full-length) and GALLS-CT (C-terminal). On some hosts, both GALLS proteins were required to substitute for VirE2. GALLS-FL localizes to the nucleus, while GALLS-CT remains cytoplasmic. GALLS-FL also interacts with IMPa-4 and with VirD2. So GALLS may aid in stabilization and/or nuclear import of VirD2/T-strand complexes. (Hodges, et al. 2004; Hodges, et al. 2006; Hodges, et al. 2009; Gelvin, 2012) |
| UniProt ID | A8VZX9 |
| KEGG ID | NA |
| PDB ID | NA |
| Domain hit(s) vs Pfam | AAA_30 [PF13604], Evalue: 1.9e-23, Aligned region: 153..321 UvrD_C_2 [PF13538], Evalue: 1.2e-06, Aligned region: 445..555 |
| Protein Sequence: 1769 a.a. [Download] |
| >gi|190404372|ref|YP_001961003.1| rcorf28 [Agrobacterium rhizogenes] MPTDDIVMSDPGMAAVDTSVPTRFQTDLRQFSNLLDESNIVEWVVNHAANRNACFGQDQLKISAARALTH YKRPVQEIDLLFQQVVASDKLTRLDSPDGQPLYTSTELRAAEQNTVRHVTNLSAEESFGVPGTIVDMVAD RGIFTPELRGALHYLSRRNRASTMVGVAGSAKTSVLGALNEAVDRFNEEALPTEKIKLIAFVPTNRAAQE LREKGLRDVATTFKAKDRTIGKNTIVVIDEMSMAKTQEIAQLIETVANATSYHPTERPKLICVGDDRQLP PVGPGDLLPIIMEQAGCYELVQPLRQLDARSRIETEKLGRDIRRDAKSAVGHYLTALEQMGLVHFVYAPP SGSNVRASDQITDRMLEEMNRVCDRYPVYDRLVMAHSNRSVNRANIALHQRFVDKSGLQDQQMSVWSSVI DSGSSESGIDEGSSRRVQQRINLVLGDRIIFSENRLQADIRNGTFATVVGLNSTEASRAENGTSAGVRIT ARLDGSGRTVTWTDSEFKGFTYGYAATIYKSQGATVDHAILLCDGALSDKLTYVGLTRHRSRLDVIASPL VANNVEALSAKLTQQSAPNNSIQFPALEPAAFQERSSVSNAHLLRPLDQMEISPTTPLSFQGRQESSFGE NQLHEPSTSDVEMPESADLYPSAIDFEVFQSSVITTEEQMQPEFCGSESTANTSAHESHQVTIATVTNLG IAIEKRKRAAAKEEIDSRKKMARHSVSSAEEVEDITSLFERLKLATSVTNTVQPTYADVDHIIGTDGRVG PIPHREDDPGSNWELSSTNAPSNVSEKRSTKDSGEKNMASNDQKMQGADSAGSERHFSQTSIVEALQAGD HKKLVETLAATGSLVEKADAGSALKAMADAYERDIKADPEKVRMLYANRRVDVDALAKEIEKQGLASGRL SGDPVSFKAFNNKDIRFRVGDRLSVTSSYRNLIAGEAGRIEKIEGKSVTFKPDGSSQSKTFEASTGEPGD RGVKGYKIEGPMTVIGSAYELTRHDDGKRVDQIYLLNDAAHGINANSALLSKADERTQLFTSLKDTADRD ILARQLGKPGSELYQDKFPALPDYTAAIDRIAKRQEVVAARGEEKTSAPIDLAPQTPPDAADAAVNNQKR IVEALQAGDHKKLVETLAATGSLVENTNLVSTLKAIAQTYERDISADPEKVRALYANKRDHVDLLAEEIE KQGLASGRLSGDPVSFKAFNNKDIRFRVGDRLSVTSSYRNLIAGEAGRIEKIEGKSVTFKPDGSSQSKTF EASTGEPGDRGVKGYKIEGPTTIIGSAYEIRNHDDGRRVDQVYVLHSAIFGSNAASALLSKADERTQVFT VQSDTADRHILASQLSKPGSELYQDKFPVSPDYAAAIDRIAKREELRAGRGEESPTVQMEPVNQTPYEAG HWTPDSQKSVVEALQAGDHKKLVETLAATGSLVEKADAGSALKAMADAYERDIKADPEKVRMLYANRRVD VDALAKEIEKQGLASGRLSGDPVSFKAFNNKDIRFRVGDRLSVTSSYRNLIAGEAGRIEKIEGKSVTFKP DGSSQSKTFEASTGEPGDRGVKGYKIEGPMTVIGSAYELTRHDDGKRVDQIYLLNDAAHGINANSALLSR ADERTQLFTSLKDTADRDILARQLGKPGSELYQDKFPALPDYTAAIDRIAKRQEVVAARGEVKPIERTEA PEIPQVQATIVGETSIIRLPDRIKTNREKPQELDVVSKEGHETTGSLRQSVQESNAAPKQTSPKAANDVD RLTRDFDERIRVRGDGRGL |
| - |
| Nucleotide Sequence: 5310 bp [Download] |
| >gi|190404344|ref|NC_010841.1|:c39693-34384 Agrobacterium rhizogenes plasmid pRi2659, complete sequence ATGCCAACCGACGACATTGTAATGTCCGATCCCGGAATGGCTGCTGTTGACACGTCTGTCCCTACGCGCT TCCAGACAGATCTTCGCCAGTTCAGTAACCTGCTGGACGAGAGCAATATTGTGGAATGGGTCGTAAACCA CGCCGCAAATCGCAATGCGTGCTTCGGACAAGACCAACTTAAGATTTCGGCGGCTCGGGCACTCACGCAC TACAAACGGCCGGTACAAGAAATCGACCTGCTATTCCAACAGGTCGTCGCAAGCGACAAACTGACCCGGC TTGATTCTCCAGATGGCCAACCTCTCTACACCTCTACAGAGCTGAGAGCTGCGGAACAGAATACTGTACG TCATGTCACCAATCTTTCCGCAGAAGAAAGCTTCGGCGTTCCGGGAACCATTGTTGATATGGTCGCCGAT AGAGGCATTTTCACACCGGAGCTCAGAGGAGCATTACATTATCTGTCTAGGCGAAATCGAGCTTCCACTA TGGTGGGCGTAGCGGGTTCCGCAAAAACAAGTGTACTTGGAGCCCTGAACGAAGCGGTGGACAGGTTCAA CGAGGAAGCGCTCCCGACCGAGAAAATAAAGTTGATTGCCTTCGTACCCACCAACCGGGCGGCCCAGGAG CTTCGTGAGAAAGGTCTTCGCGACGTCGCGACGACTTTCAAGGCGAAAGACCGCACCATCGGAAAAAATA CCATCGTCGTGATCGACGAAATGTCGATGGCCAAGACGCAGGAGATCGCGCAACTAATCGAGACAGTTGC AAATGCCACTTCCTATCACCCAACCGAAAGGCCGAAACTGATATGTGTGGGAGACGATCGGCAACTGCCC CCTGTTGGACCTGGTGATCTGCTTCCGATTATTATGGAGCAAGCCGGCTGCTATGAACTCGTCCAGCCGC TTCGACAGCTCGACGCGCGGTCGCGAATAGAAACAGAAAAGCTCGGACGTGACATACGACGCGATGCCAA GAGCGCCGTCGGGCACTATCTCACAGCGCTCGAACAAATGGGCCTCGTCCATTTCGTATATGCTCCTCCT AGCGGCTCAAACGTCAGGGCGTCAGACCAGATCACTGACCGCATGCTGGAGGAGATGAATAGAGTTTGCG ACCGATATCCGGTGTATGATCGTCTTGTGATGGCACACTCCAACAGGAGCGTTAATCGCGCCAATATCGC TCTTCATCAGAGGTTTGTTGACAAATCCGGATTGCAAGACCAGCAAATGAGTGTGTGGTCAAGCGTAATA GATAGTGGAAGTTCGGAATCTGGAATAGATGAGGGTTCGTCGAGACGCGTTCAGCAAAGGATTAATCTCG TCTTGGGAGACAGGATTATATTCAGCGAAAACCGTCTGCAAGCGGACATCCGCAATGGTACTTTTGCAAC TGTAGTCGGACTTAATAGCACAGAAGCTTCCCGAGCTGAGAATGGCACATCTGCTGGGGTTCGCATTACT GCAAGGTTAGACGGATCTGGACGAACCGTCACTTGGACCGATAGCGAGTTTAAGGGTTTTACCTACGGCT ATGCTGCTACAATTTATAAGAGTCAGGGGGCCACAGTTGATCACGCCATTCTCCTCTGTGATGGTGCTCT TTCAGATAAGCTGACCTATGTCGGCCTGACGAGGCATCGCAGTCGTCTCGACGTCATTGCTTCTCCCCTC GTGGCAAACAATGTTGAGGCTCTCAGTGCAAAGCTCACTCAGCAAAGCGCGCCAAATAATTCCATTCAAT TCCCAGCGCTCGAACCTGCTGCCTTCCAAGAGCGCTCGTCGGTTTCGAACGCGCACTTGCTAAGGCCGCT CGACCAGATGGAAATCTCTCCAACGACGCCGCTTTCCTTCCAAGGCAGACAAGAAAGCTCCTTCGGGGAG AACCAGCTGCACGAACCATCCACATCGGATGTGGAAATGCCGGAAAGCGCGGACCTTTACCCGAGCGCCA TTGACTTCGAAGTTTTCCAGTCTTCTGTCATCACGACTGAAGAGCAAATGCAACCGGAATTCTGCGGGTC GGAATCGACGGCTAACACCTCAGCCCACGAAAGCCACCAAGTCACGATCGCAACGGTAACAAACCTGGGG ATAGCGATCGAGAAACGGAAACGAGCGGCGGCAAAGGAAGAAATCGATTCTCGCAAAAAAATGGCCCGTC ACTCAGTTAGTTCTGCCGAGGAAGTTGAAGATATCACTTCCCTGTTCGAACGATTGAAACTGGCAACCTC GGTCACCAACACAGTTCAACCGACGTATGCGGACGTCGATCATATCATCGGTACTGATGGCCGAGTGGGT CCAATACCGCACCGGGAAGATGATCCGGGATCAAATTGGGAATTGTCGTCAACGAACGCCCCCTCAAACG TCTCAGAGAAACGATCGACCAAAGACTCAGGAGAAAAAAATATGGCTTCGAATGACCAGAAAATGCAAGG CGCTGACTCTGCAGGCAGCGAGCGTCATTTTTCGCAGACTTCCATTGTGGAAGCCCTGCAGGCTGGCGAT CACAAGAAGCTCGTGGAAACGCTCGCCGCAACCGGCTCCCTTGTCGAGAAGGCGGACGCCGGGTCGGCGC TGAAAGCAATGGCGGATGCTTACGAAAGGGATATCAAGGCAGATCCCGAAAAGGTCCGCATGCTCTATGC CAATCGGCGCGTTGATGTAGATGCGCTGGCCAAGGAGATCGAAAAACAGGGCCTTGCATCCGGTCGCCTG AGTGGCGACCCTGTCAGCTTCAAGGCGTTCAACAACAAGGATATCCGTTTCAGGGTCGGCGACAGGCTCA GCGTCACCAGCAGCTACAGGAATCTCATTGCCGGTGAAGCCGGCCGGATCGAAAAGATCGAAGGCAAATC CGTTACCTTCAAACCCGATGGCAGCAGTCAGAGCAAGACCTTCGAGGCCAGCACCGGCGAGCCGGGTGAC AGGGGCGTCAAGGGCTACAAAATCGAAGGCCCGATGACGGTCATCGGCTCCGCCTATGAACTTACGCGAC ATGATGACGGCAAACGTGTTGACCAAATCTATCTCTTGAACGACGCTGCCCATGGCATCAACGCCAATAG CGCTTTGCTGAGCAAGGCCGACGAGCGGACGCAGCTTTTCACTTCGCTCAAGGACACCGCCGACAGGGAC ATCTTGGCCAGGCAACTGGGAAAGCCGGGCAGCGAGCTCTACCAGGACAAATTCCCAGCCTTGCCGGACT ACACAGCCGCCATCGATCGGATTGCAAAACGCCAGGAAGTTGTCGCGGCACGTGGCGAGGAGAAAACTTC CGCCCCGATAGACCTGGCGCCCCAAACGCCGCCCGACGCTGCCGATGCGGCAGTCAATAACCAGAAAAGA ATCGTCGAGGCCTTGCAGGCAGGCGACCACAAGAAGCTTGTGGAAACGCTAGCCGCAACCGGCTCCCTTG TTGAGAACACAAATCTCGTATCAACGTTGAAAGCGATAGCGCAGACTTATGAAAGAGACATTAGTGCGGA CCCCGAGAAAGTCCGCGCTCTCTACGCCAACAAACGTGATCATGTAGATTTGCTTGCCGAAGAGATCGAA AAGCAGGGCCTTGCATCCGGTCGCCTGAGTGGCGACCCTGTCAGCTTCAAGGCGTTTAACAACAAGGATA TCCGTTTCAGGGTCGGTGACAGGCTCAGCGTCACTAGCAGCTATAGGAATCTCATTGCCGGTGAAGCCGG CCGGATCGAAAAGATCGAAGGCAAATCCGTTACCTTCAAACCCGATGGCAGCAGTCAGAGCAAGACCTTC GAGGCCAGCACCGGCGAGCCGGGTGACAGGGGTGTCAAGGGCTATAAGATTGAGGGGCCCACGACAATCA TCGGCTCCGCCTACGAAATCAGAAATCACGACGATGGCAGGCGCGTCGATCAGGTTTATGTCTTGCACAG TGCCATTTTCGGCTCAAATGCCGCGAGCGCCCTGTTGAGCAAGGCCGACGAACGAACCCAGGTTTTCACC GTGCAAAGCGACACGGCTGACAGACACATCTTGGCCAGCCAACTCAGCAAGCCTGGCAGCGAGCTCTATC AGGACAAATTCCCAGTCTCGCCGGACTACGCAGCCGCGATCGACCGGATCGCGAAACGCGAGGAACTTAG GGCCGGACGTGGCGAGGAAAGTCCTACTGTTCAAATGGAGCCGGTGAACCAAACGCCCTACGAGGCGGGA CATTGGACGCCTGACAGTCAAAAATCTGTGGTGGAAGCCCTGCAGGCTGGCGATCACAAGAAGCTCGTGG AAACGCTCGCCGCAACCGGCTCCCTTGTCGAGAAAGCGGACGCCGGGTCGGCGCTGAAAGCAATGGCGGA TGCTTACGAAAGGGATATCAAGGCAGATCCCGAAAAGGTCCGCATGCTCTATGCCAATCGGCGCGTTGAT GTAGATGCGCTGGCCAAGGAGATCGAAAAACAGGGCCTTGCATCCGGTCGCCTGAGTGGCGACCCTGTCA GCTTCAAGGCGTTCAACAACAAGGATATCCGTTTCAGGGTCGGCGACAGGCTCAGCGTCACCAGCAGCTA CAGGAATCTCATTGCCGGTGAAGCCGGCCGGATCGAAAAGATCGAAGGCAAATCCGTTACCTTCAAACCC GATGGCAGCAGTCAGAGCAAGACCTTCGAGGCCAGCACCGGCGAGCCGGGTGACAGGGGCGTCAAGGGCT ACAAAATCGAAGGCCCGATGACGGTCATCGGCTCCGCCTATGAACTTACGCGACATGATGACGGCAAACG TGTTGACCAAATCTATCTCTTGAACGACGCTGCCCATGGCATCAACGCCAATAGCGCTTTGCTGAGCAGG GCCGACGAGCGGACGCAGCTTTTCACTTCGCTCAAGGACACCGCCGACAGGGACATCTTGGCCAGGCAAC TGGGAAAGCCGGGCAGCGAGCTCTACCAGGACAAATTCCCAGCCTTGCCGGACTACACAGCCGCCATCGA TCGGATTGCAAAACGCCAGGAAGTTGTCGCGGCACGTGGCGAGGTCAAGCCGATTGAGCGCACCGAAGCG CCCGAAATACCCCAGGTTCAAGCTACTATCGTTGGCGAGACCTCGATCATTCGGTTGCCCGATCGGATAA AAACCAATCGCGAGAAGCCCCAAGAACTTGATGTAGTCTCGAAAGAAGGGCACGAAACTACAGGTAGTCT GAGGCAGAGTGTCCAAGAGAGTAATGCAGCACCAAAACAGACTTCGCCGAAGGCGGCAAATGACGTGGAT CGGCTGACACGGGATTTTGACGAGCGCATCCGCGTCCGCGGGGATGGACGTGGACTCTAA |
| Reference |
(1) Hodges LD; Lee LY; McNett H; Gelvin SB; Ream W (2009). The Agrobacterium rhizogenes GALLS gene encodes two secreted proteins required for genetic transformation of plants. J Bacteriol. 191(1):355-64. [PudMed:18952790] 
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| experimental literature |