| Effector Protein ID: 16119798 |
 T4SS name [ID] | VirB [19] |
| Gene symbol | virD2 |
| Locus tag | Atu6182 |
| Strain | Agrobacterium tumefaciens str. C58 |
| Replicon | plasmid Ti [GenBank: NC_003065] [Browse all T4SS(s) in this replicon] |
| Location [Strand] | 196503..197846 [+] |
| Product | type IV secretion system T-DNA border endonuclease VirD2 |
| Effector type | Protein |
| Descripton | VirD2 |
| T4CP requirement | T4CP Depedent |
| Target/Effection | VirD2 is an endonuclease that nicks the T-DNA region of Ti-/Ri-plasmids at border repeat sequences flanking T-DNA. During T-DNA processing, VirD2 covalently attaches to the 5′ end of the resulting T-strand. VirD2 serves as a “pilot protein” to lead T-strands through the T4SS and into the host cell. Once in the host, VirD2 probably plays a major role in targeting T-strands to the nucleus |
| UniProt ID | P18592 |
| KEGG ID | atu:Atu6182 |
| PDB ID | NA |
| Domain hit(s) vs Pfam | Relaxase [PF03432], Evalue: 7.3e-34, Aligned region: 16..213 |
| Protein Sequence: 447 a.a. [Download] |
| >gi|16119798|ref|NP_396503.1| endonuclease [Agrobacterium tumefaciens str. C58] MPDRAQVIIRIVPGGGTKTLQQIINQLEYLSRKGRLELQRSARHLDIPLPPDQIHELARSWVQETGTYDE SQPDEERQQELTTHIIVSFPAGTSQVAAYAASREWAAEMFGSGAGGGRYNYLTAFHIDRDHPHLHVVVNR RELLGHGWLKISRRHPQLNYDALRIKMAEISLRHGIALDASRRAERGITERPITYAQYRRLEREQARQIR FEDADLEQSSPQGDHPEFSQPFDTSPFEASAGGPEDMPRPNNRQNESQVHLQEPAGVSNEAGVLVRVALE TERLAQPFVSETILADDIGSGSSRVAEGRVESANRTPDIPRAATEAATHTTHDRQRRAKRPHDDDGGPSG AKRVTLEGIAVGPQANAGEQDGSSGPLVRQAGTSRPSPPTATTRASTATDSLSATAHLQQRRGVLSKRPR EDDDGEPSERKRERDERSKDGRGGNRR |
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| Nucleotide Sequence: 1344 bp [Download] |
| >gi|159161952|ref|NC_003065.3|:196503-197846 Agrobacterium tumefaciens str. C58 plasmid Ti, complete sequence ATGCCCGATCGAGCTCAAGTTATCATTCGCATTGTGCCGGGAGGTGGCACCAAGACCCTTCAACAAATTA TCAATCAGTTGGAGTATCTATCCCGGAAGGGCAGGCTGGAGCTGCAGCGTTCAGCCCGACATCTCGATAT TCCCCTGCCACCGGATCAAATCCACGAACTTGCCCGAAGCTGGGTTCAAGAGACTGGAACTTATGACGAA AGTCAGCCAGACGAGGAAAGGCAACAGGAGTTGACCACCCATATTATTGTAAGCTTCCCCGCCGGTACAA GCCAGGTAGCGGCTTATGCGGCGAGCCGGGAGTGGGCAGCCGAGATGTTTGGGTCAGGCGCAGGGGGGGG CCGATACAACTATCTTACGGCCTTCCACATCGATCGCGACCACCCACATCTGCATGTCGTCGTCAATCGG CGCGAACTTTTAGGACACGGCTGGCTGAAGATATCTCGGCGCCATCCCCAACTGAATTACGACGCCCTGC GCATAAAGATGGCCGAGATTTCACTTCGTCATGGCATTGCCCTCGATGCGAGCCGACGAGCAGAACGTGG CATCACCGAGCGGCCGATCACTTATGCCCAATATCGGCGCCTTGAGCGGGAGCAGGCTCGCCAAATCCGT TTCGAAGACGCGGATTTGGAACAGTCGTCGCCGCAAGGAGATCATCCAGAATTCAGCCAACCTTTCGATA CATCCCCATTTGAAGCATCCGCGGGCGGACCGGAGGACATGCCTCGGCCCAACAATCGGCAGAATGAGTC GCAAGTTCATCTCCAGGAGCCAGCTGGTGTCAGCAACGAAGCCGGTGTCCTTGTGCGGGTTGCATTGGAG ACGGAGCGCCTTGCTCAACCATTCGTTTCCGAAACCATTCTCGCGGACGACATAGGGAGCGGCTCTTCGC GTGTTGCCGAGGGCCGTGTGGAGAGCGCAAACCGCACTCCCGATATTCCTCGCGCAGCAACTGAAGCTGC CACGCACACGACACACGACCGGCAGCGGCGTGCAAAGCGTCCTCATGATGACGACGGAGGGCCGAGTGGA GCAAAACGTGTGACATTGGAAGGCATCGCGGTTGGGCCCCAGGCGAACGCCGGCGAACAGGATGGCAGTA GTGGCCCCTTAGTACGGCAAGCTGGAACGTCTCGGCCATCTCCACCGACGGCCACGACGCGGGCCAGCAC CGCAACCGATTCATTGTCTGCTACAGCCCACCTCCAGCAACGGAGAGGTGTCCTTTCAAAGCGTCCGCGT GAAGATGATGATGGAGAACCGAGTGAACGCAAACGCGAGAGAGATGAGCGCAGCAAGGACGGGCGTGGGG GAAATAGGAGATAG |
| Reference |
(1) Aguilar J; Zupan J; Cameron TA; Zambryski PC (2010). Agrobacterium type IV secretion system and its substrates form helical arrays around the circumference of virulence-induced cells. Proc Natl Acad Sci U S A. 107(8):3758-63. [PudMed:20133577] 
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| experimental literature |