| Effector Protein ID: 10954814 |
 T4SS name [ID] | VirB [20] |
| Gene symbol | riorf168 |
| Locus tag | pRi1724_p169 |
| Strain | Agrobacterium rhizogenes plasmid pRi1724 (NC_002575) |
| Replicon | plasmid pRi1724 [GenBank: NC_002575] [Browse all T4SS(s) in this replicon] |
| Location [Strand] | 206225..207571 [+] |
| Product | hypothetical protein |
| Effector type | Protein |
| Descripton | VirD2 |
| T4CP requirement | T4CP Depedent |
| Target/Effection | VirD2 is an endonuclease that nicks the T-DNA region of Ti-/Ri-plasmids at border repeat sequences flanking T-DNA. During T-DNA processing, VirD2 covalently attaches to the 5' end of the resulting T-strand. VirD2 serves as a "pilot protein" to lead T-strands through the T4SS and into the host cell. Once in the host, VirD2 probably plays a major role in targeting T-strands to the nucleus |
| UniProt ID | Q9F587 |
| KEGG ID | NA |
| PDB ID | NA |
| Domain hit(s) vs Pfam | Relaxase [PF03432], Evalue: 2.8e-32, Aligned region: 16..207 |
| Protein Sequence: 448 a.a. [Download] |
| >gi|10954814|ref|NP_066749.1| riorf168 [Agrobacterium rhizogenes] MPDRSQVIIRIVPGGGTKTLQQIINQFEYLSRKGKLELQRSARHLDIFVPPDEIRELARSWLQETGTYHE SQPDEERQQDLTTHIIVSFPAGTSQVAAYAASREWAAEMFGSGAGGGRYNYLTAFHIDRDHPHLHVVVNR RELLGHGWLKISRRHPQLNYDALRIKMSEISLRHGVVLEATRRAERGITERPMTFAQYRRLERQQANQIR FEDPEFEEFSPGGNDREPDQSFNSSHGEPPQNVSVSSERNECLQPVSEARSRAPIGSNGNGVTPDRVPSE NDSRSQSGSGEAFMDDDNLRNGPLTIAGDGQDLEGRSGIHRLATEPVTHTTSEDDVRQRPHRKRPRGDEE EQSGAKLTRVNGVRTGVTISDVPVAQDDPITSPIQPPGPNPLHDPGQTSIATDALPPTADRPQQGEPNSK RARDDDAEPSIRKRSRDGRSQGDEGNRR |
| - |
| Nucleotide Sequence: 1347 bp [Download] |
| >gi|10954646|ref|NC_002575.1|:206225-207571 Agrobacterium rhizogenes plasmid pRi1724 DNA, complete sequence ATGCCCGATCGGTCTCAAGTCATCATCCGCATTGTGCCGGGAGGTGGGACGAAGACCCTCCAACAGATTA TCAATCAGTTTGAGTATCTATCCCGGAAGGGCAAGTTGGAGCTGCAGCGCTCGGCCCGGCATCTCGATAT TTTCGTACCACCGGATGAAATCCGCGAGCTCGCCCGAAGCTGGCTTCAAGAAACCGGGACTTATCACGAA AGTCAGCCAGACGAGGAAAGGCAACAGGATTTGACCACCCACATTATTGTAAGCTTCCCTGCCGGTACAA GTCAGGTAGCAGCTTATGCAGCGAGCCGGGAGTGGGCAGCCGAGATGTTTGGGTCAGGCGCAGGGGGAGG CCGATACAACTATCTTACAGCCTTTCACATCGATCGCGATCACCCACATCTGCATGTGGTCGTCAACCGT CGCGAACTTTTGGGGCACGGCTGGCTGAAAATATCCCGGCGCCACCCCCAACTGAATTACGACGCCTTGC GCATTAAAATGTCCGAGATTTCACTTCGCCATGGCGTCGTCCTCGAGGCGACCAGGCGAGCTGAGCGCGG CATCACCGAGCGGCCGATGACTTTTGCCCAGTATCGACGCCTTGAGCGACAACAGGCCAATCAAATTCGT TTCGAGGATCCTGAGTTCGAAGAATTCTCGCCTGGGGGAAACGACCGAGAACCGGATCAATCTTTCAATT CTTCGCACGGTGAGCCGCCTCAAAACGTGTCAGTAAGTTCGGAACGAAACGAGTGTCTGCAACCGGTGTC TGAAGCGCGTTCCCGGGCACCAATCGGATCGAACGGCAACGGCGTGACGCCGGATCGTGTACCATCAGAA AACGATTCCCGTTCTCAATCCGGTAGCGGTGAAGCCTTTATGGACGATGACAATTTGAGGAACGGCCCTC TCACCATCGCCGGAGACGGCCAGGATCTTGAAGGCCGTTCCGGCATTCATCGTTTGGCAACCGAACCCGT CACGCACACAACAAGCGAAGATGACGTTCGGCAACGGCCCCATCGAAAGCGGCCTCGCGGTGATGAGGAA GAGCAGAGCGGCGCAAAACTGACCAGGGTAAACGGAGTTCGGACGGGAGTGACAATCAGCGACGTACCGG TTGCCCAGGACGATCCGATTACATCGCCGATCCAGCCCCCTGGACCAAATCCGTTGCATGACCCGGGACA GACCAGCATCGCTACGGACGCATTGCCTCCGACAGCCGATCGCCCGCAGCAGGGAGAGCCAAATTCTAAG CGTGCTCGTGATGATGACGCCGAGCCGAGCATTCGCAAGCGGTCAAGGGACGGGCGCAGCCAAGGGGATG AGGGAAACAGAAGGTAG |
| Reference |
(1) Hodges LD; Cuperus J; Ream W (2004). Agrobacterium rhizogenes GALLS protein substitutes for Agrobacterium tumefaciens single-stranded DNA-binding protein VirE2. J Bacteriol. 186(10):3065-77. [PudMed:15126468] 
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| experimental literature |