SecReT4
Effector Protein ID: 10954701
T4SS name [ID]VirB [20]
Gene symbolriorf55
Locus tagpRi1724_p056
StrainAgrobacterium rhizogenes plasmid pRi1724 (NC_002575)
Repliconplasmid pRi1724 [GenBank: NC_002575] [Browse all T4SS(s) in this replicon]
Location [Strand]63178..68466 [-]
Producthypothetical protein
Effector typeProtein
DescriptonGALLS
T4CP requirementT4CP Depedent
Target/EffectionGALLS can substitute for VirE2 in strains of Agrobacterium rhizogenes that lack virE2. GALLS can be made in two forms: GALLS-FL (full-length) and GALLS-CT (C-terminal). On some hosts, both GALLS proteins were required to substitute for VirE2. GALLS-FL localizes to the nucleus, while GALLS-CT remains cytoplasmic. GALLS-FL also interacts with IMPa-4 and with VirD2. So GALLS may aid in stabilization and/or nuclear import of VirD2/T-strand complexes. (Hodges, et al. 2004; Hodges, et al. 2006; Hodges, et al. 2009; Gelvin, 2012)
UniProt IDQ9KWC9
KEGG IDNA
PDB IDNA
Domain hit(s) vs Pfam AAA_30 [PF13604], Evalue: 2.4e-23, Aligned region: 146..313
UvrD_C_2 [PF13538], Evalue: 1.2e-06, Aligned region: 438..548
Protein Sequence: 1762 a.a.     [Download]
>gi|10954701|ref|NP_066636.1| hypothetical protein [Agrobacterium rhizogenes]
MSDPGMAAVDTSVPMRFQTDLRQFSNLLDESNIVGWVVNHAANRNACFGQDQLKISAARALTHYKRPVQE
IDLLFQQVVASDKLTRLDSPDGQPLYTSTELRAAEQNTVRHVTNLSAEESFGVPGTIVDMVADRSIFTPE
LRGALHYLSRRNRASTMVGVAGSAKTSVLGALNEAVDRFNEEALPTEKIKLIAFVPTNRAAQELREKGLR
DVATTFKAKDRTIGKNTIVVIDEMSMAKTQEIAQLIETVANATSYHPTERPKLICVGDDRQLPPVGPGDL
LPIIMEQAGCYELVQPLRQLDARSRIETEKLGRDIRRDAKSAVGHYLTAIEQMGLVHFVYAPPGGSNVRA
SDQITDRMLEEMNRVCDRYPVDDRLVMAHSNRSVNRANIALHQRFVDKSGLQDQQMSVWSSVIDSGSSES
GIDEGSSRRVQQRINLVLGDRIIFSENRLQADIRNGTFATVVGLNSTEPSRAENGTSAGVRITARLDGSG
RTVTWTDSEFKGFTYGYAATIYKSQGATVDHAILLCDGALSDKLTYVGLTRHRSRLDVIASPLVANNVEA
LSAKLTQQSAPNNSIQFPALEPAAFQERSSVSNAHLLRPLDQMEISPTTPLSFQGRQESSFGENQLHEPS
TSDVEMPESADLYPSAIDFEVFQSSVITTEEQMQPEFCGSESTANTSAHESHQVTIATVTNLGIAIEKRK
RAAAKEEIDSRKKMARHSVSSAEEVEDITSLFERLKLATSVTNTVQPTYADVDHIIGTDGRVGPIPHRED
DPGSNWELSSTNAPSNVSEKRSTKDSGEKNMASNDQKMQGADSAGSERHFSQTSIVEALQAGDHKKLVET
LAATGSLVEKADAGSALKAMADAYERDIKADPEKVRMLYANRRVDVDALAKEIEKQGLASGRLSGDPVSF
KAFNNKDIRFRVGDRLSVTSSYRNLIAGEAGRIEKIEGKSVTFKPDGSSQSKTFEASTGEPGDRGVKGYK
IEGPMTVIGSAYELTRHDDGKRVDQIYLLNDAAHGINANGALLSKADERTQLFTSLKDTADRDILARQLG
KPGSELYQDKFPALPDYTAAIDRIAKRQEVVAARGEEKTSTPIDLAPQTPPDAADAAVNNQKRIVEALQA
GDHKKLVETLAATGSLVENTNLVSTLKAIAQTYERDISADPEKVRALYANKRDHVDLLAEEIEKQGLASG
RLSGDPVSFKAFNNKDIRFRVGDRLSVTSSYRNLIAGEAGRIEKIEGKSVTFKPDGSSQSKTFEASTGEP
GDRGVKGYKIEGPTTIIGSAYEIRNHDDGRRVDQVYVLHSAIFGSNAASALLSKADERTQVFTVQSDTAD
RHILASQLSKPGSELYQDKFPVSPDYAAAIDRIAKREELRAGRGEESPTVQMEPVNQTPYEVGHWTPDSQ
KSVVEALQAGDHKKLVETLAATGSLVEKADAGSALKAMADAYERDIKADPEKVRMLYANRRVDVDALAKE
IEKQGLASGRLSGDPVSFKAFNNKDIRFRVGDRLSVTSSYRNLIAGEAGRIEKIEGKSVTFKPDGSSQSK
TFEASTGEPGDRGVKGYKIEGPMTVIGSAYELTRHDDGKRVDQIYLLNDAAHGINANGALLSKADERTQL
FTSLKDTADRDILARQLGKPGSELYQDKFPALPDYTAAIDRIAKRQEVVAARGEIKPIERTEAPEIPQVQ
ATIVGETSIIRLPDRIKTNREKPQELDVVSKEGHETTGSLRQSVQESNAAPKQTSPKAANDVDRLTRDFD
ERIRVRGDGRGL
-
Nucleotide Sequence: 5289 bp     [Download]
>gi|10954646|ref|NC_002575.1|:c68466-63178 Agrobacterium rhizogenes plasmid pRi1724 DNA, complete sequence
ATGTCCGATCCCGGAATGGCTGCTGTTGACACGTCTGTCCCTATGCGCTTCCAGACAGATCTTCGCCAGT
TCAGTAACCTGCTGGACGAGAGCAATATTGTGGGATGGGTCGTAAACCACGCCGCAAATCGCAATGCGTG
CTTCGGACAAGACCAACTTAAGATTTCGGCGGCTCGGGCACTCACGCACTACAAACGGCCGGTACAAGAA
ATCGACCTGCTATTCCAACAGGTCGTCGCAAGCGACAAACTGACCCGGCTTGATTCTCCAGATGGCCAAC
CTCTCTACACCTCTACAGAGCTGAGAGCTGCGGAACAGAATACTGTACGTCATGTCACTAATCTTTCCGC
AGAAGAAAGCTTCGGCGTTCCGGGAACCATTGTTGATATGGTCGCCGATAGAAGCATTTTCACACCGGAG
CTCAGAGGAGCATTACATTATCTGTCTAGGCGAAATCGAGCTTCCACTATGGTGGGCGTAGCGGGTTCCG
CAAAAACAAGTGTACTTGGAGCCCTGAACGAAGCGGTGGACAGGTTCAACGAGGAAGCGCTCCCGACCGA
GAAAATAAAGTTGATTGCCTTCGTACCCACCAACCGGGCGGCCCAGGAGCTTCGTGAGAAAGGTCTTCGC
GACGTCGCGACGACTTTCAAGGCGAAAGACCGCACCATCGGAAAAAATACCATCGTCGTGATCGACGAAA
TGTCGATGGCCAAGACGCAGGAGATCGCGCAACTAATCGAGACAGTTGCAAATGCCACTTCCTATCACCC
AACCGAAAGGCCGAAACTGATATGTGTGGGAGACGATCGGCAACTGCCCCCTGTTGGACCTGGTGATCTG
CTTCCGATTATTATGGAGCAAGCCGGCTGCTATGAACTCGTCCAGCCGCTTCGACAGCTCGACGCGCGGT
CGCGAATAGAAACAGAAAAGCTCGGACGTGACATACGACGCGATGCCAAGAGCGCCGTCGGGCACTATCT
CACAGCGATCGAACAAATGGGCCTCGTCCATTTCGTATATGCTCCTCCTGGCGGCTCAAACGTCAGGGCG
TCAGACCAGATCACTGACCGCATGCTGGAGGAGATGAATAGAGTTTGCGACCGATATCCGGTGGATGATC
GTCTTGTGATGGCACACTCCAACAGGAGCGTTAATCGCGCCAATATCGCTCTTCATCAGAGGTTTGTTGA
CAAATCCGGATTGCAAGACCAGCAAATGAGTGTGTGGTCAAGCGTAATAGATAGTGGAAGTTCGGAATCT
GGAATAGATGAGGGTTCGTCGAGACGCGTTCAGCAAAGGATTAATCTCGTCTTGGGAGACAGGATTATAT
TCAGCGAAAACCGTCTGCAAGCGGACATCCGCAATGGTACTTTTGCAACTGTAGTCGGACTTAATAGCAC
AGAACCTTCCCGAGCTGAGAATGGCACATCTGCTGGGGTTCGCATTACTGCAAGGCTAGACGGATCTGGA
CGAACCGTCACTTGGACCGATAGCGAGTTTAAGGGTTTTACCTACGGCTATGCTGCTACAATTTATAAGA
GTCAGGGGGCCACAGTTGATCACGCCATTCTCCTCTGTGATGGTGCTCTTTCAGATAAGCTGACCTATGT
CGGCCTGACGAGGCATCGCAGTCGTCTCGACGTCATTGCTTCTCCCCTCGTGGCAAACAATGTTGAGGCT
CTCAGTGCAAAGCTCACTCAGCAAAGCGCGCCAAATAATTCCATTCAATTCCCGGCGCTCGAACCTGCTG
CCTTCCAAGAGCGCTCGTCGGTTTCGAACGCGCACTTGCTAAGGCCGCTCGACCAGATGGAAATCTCTCC
AACGACGCCGCTTTCCTTCCAAGGCAGACAAGAAAGCTCCTTCGGGGAGAACCAGCTGCACGAACCATCC
ACATCGGATGTGGAAATGCCGGAAAGCGCGGACCTTTACCCGAGCGCCATTGACTTCGAAGTTTTCCAGT
CTTCTGTCATCACGACTGAAGAGCAAATGCAACCGGAATTCTGCGGGTCGGAATCGACGGCTAACACCTC
AGCCCACGAAAGCCACCAAGTCACGATCGCAACGGTAACAAACCTGGGGATAGCGATCGAGAAACGGAAA
CGAGCGGCGGCAAAGGAAGAAATCGATTCTCGCAAAAAAATGGCCCGTCACTCAGTTAGTTCTGCCGAGG
AAGTTGAAGATATCACTTCCCTATTCGAACGATTGAAACTGGCAACCTCGGTCACGAACACAGTTCAACC
GACGTATGCGGACGTCGATCATATCATCGGTACTGATGGCCGAGTGGGTCCAATACCGCACCGGGAAGAT
GATCCGGGATCAAATTGGGAATTGTCGTCAACGAACGCCCCCTCAAACGTCTCAGAGAAACGATCGACCA
AAGACTCAGGAGAAAAAAATATGGCTTCGAATGACCAGAAAATGCAAGGCGCTGACTCTGCAGGCAGCGA
GCGTCATTTTTCGCAGACTTCCATTGTGGAAGCCCTGCAGGCTGGCGATCACAAGAAGCTCGTGGAAACG
CTCGCCGCAACCGGCTCCCTTGTCGAGAAGGCGGACGCCGGGTCGGCGCTGAAAGCAATGGCGGATGCTT
ACGAAAGGGATATCAAGGCAGATCCCGAAAAGGTCCGCATGCTCTATGCCAATCGGCGCGTTGATGTAGA
TGCGCTGGCCAAGGAGATCGAAAAACAGGGCCTTGCATCCGGTCGCCTGAGTGGCGACCCTGTCAGCTTC
AAGGCGTTCAACAACAAGGATATCCGTTTCAGGGTCGGCGACAGGCTCAGCGTCACCAGCAGCTACAGGA
ATCTCATTGCCGGTGAAGCCGGCCGGATCGAAAAGATCGAAGGCAAATCCGTTACCTTCAAACCCGATGG
CAGCAGTCAGAGCAAGACCTTCGAGGCCAGCACCGGCGAGCCGGGTGACAGGGGCGTCAAGGGCTACAAA
ATCGAAGGCCCGATGACGGTCATCGGCTCCGCCTATGAACTTACGCGACATGATGACGGCAAACGTGTTG
ACCAAATCTATCTCTTGAACGACGCTGCCCATGGCATCAACGCCAATGGCGCCTTGCTGAGCAAGGCCGA
CGAGCGGACGCAGCTTTTCACTTCGCTCAAGGACACCGCCGACAGAGACATCTTGGCCAGGCAACTGGGA
AAGCCGGGCAGCGAGCTCTACCAGGACAAATTCCCAGCCTTGCCGGACTACACAGCCGCCATCGATCGGA
TTGCAAAACGCCAGGAAGTTGTCGCGGCACGTGGCGAGGAGAAAACTTCCACCCCGATAGATCTGGCGCC
CCAAACGCCGCCCGACGCTGCCGATGCGGCAGTCAATAACCAGAAAAGAATCGTCGAGGCCTTGCAGGCA
GGCGACCACAAGAAGCTTGTGGAAACGCTAGCCGCAACCGGCTCCCTTGTTGAGAACACAAATCTCGTAT
CAACGTTGAAAGCGATAGCGCAGACTTATGAAAGAGACATTAGTGCGGACCCCGAGAAAGTCCGCGCTCT
CTACGCCAACAAACGTGATCATGTAGATTTGCTTGCCGAAGAGATCGAAAAGCAGGGCCTTGCATCCGGT
CGCCTGAGTGGCGACCCTGTCAGCTTCAAGGCGTTTAACAACAAGGATATCCGTTTCAGGGTCGGTGACA
GGCTCAGCGTCACCAGCAGCTATAGGAATCTCATTGCCGGTGAAGCCGGCCGGATCGAAAAGATCGAAGG
CAAATCCGTTACCTTCAAACCCGATGGCAGCAGTCAGAGCAAGACCTTCGAGGCCAGCACCGGCGAGCCG
GGTGACAGGGGTGTCAAGGGCTATAAGATTGAGGGGCCCACGACAATCATCGGCTCCGCCTACGAAATCA
GAAATCACGACGATGGCAGGCGCGTCGATCAGGTTTATGTCTTGCACAGTGCCATTTTCGGCTCAAATGC
CGCGAGCGCCCTGTTGAGCAAGGCCGACGAACGAACCCAGGTTTTCACCGTGCAAAGCGACACGGCTGAC
AGACACATCTTGGCCAGCCAACTAAGCAAGCCTGGCAGCGAGCTCTATCAGGACAAATTCCCAGTCTCGC
CGGACTACGCAGCCGCGATCGACCGGATCGCGAAACGCGAGGAACTTAGGGCCGGACGTGGCGAGGAAAG
TCCTACTGTTCAAATGGAGCCGGTGAACCAAACGCCCTACGAGGTGGGACATTGGACGCCTGACAGTCAA
AAATCTGTGGTGGAAGCCCTGCAGGCTGGCGATCACAAGAAGCTCGTGGAAACGCTCGCCGCAACCGGCT
CCCTTGTCGAGAAGGCGGACGCCGGGTCGGCGCTGAAAGCAATGGCGGATGCTTACGAAAGGGATATCAA
GGCAGATCCCGAAAAGGTCCGCATGCTCTATGCCAATCGGCGCGTTGATGTAGATGCGCTGGCCAAGGAG
ATCGAAAAACAGGGCCTTGCATCCGGTCGCCTGAGTGGCGACCCTGTCAGCTTCAAGGCGTTCAACAACA
AGGATATCCGTTTCAGGGTCGGCGACAGGCTCAGCGTCACCAGCAGCTACAGGAATCTCATTGCCGGTGA
AGCCGGCCGGATCGAAAAGATCGAAGGCAAATCCGTTACCTTCAAACCCGATGGCAGCAGTCAGAGCAAG
ACCTTCGAGGCCAGCACCGGCGAGCCGGGTGACAGGGGCGTCAAGGGCTACAAAATCGAAGGCCCGATGA
CGGTCATCGGCTCCGCCTATGAACTTACGCGACATGATGACGGCAAACGTGTTGACCAAATCTATCTCTT
GAACGACGCTGCCCATGGCATCAACGCCAATGGCGCCTTGCTGAGCAAGGCCGACGAGCGGACGCAGCTT
TTCACTTCGCTCAAGGACACCGCCGACAGAGACATCTTGGCCAGGCAACTGGGAAAGCCGGGCAGCGAGC
TCTACCAGGACAAATTCCCAGCCTTGCCGGACTACACAGCCGCCATCGATCGGATTGCAAAACGCCAGGA
AGTTGTCGCGGCACGTGGCGAGATCAAGCCGATTGAGCGCACCGAAGCGCCCGAAATACCCCAGGTTCAA
GCTACTATCGTTGGCGAGACCTCGATCATTCGGTTGCCCGATCGGATAAAAACCAATCGCGAGAAGCCCC
AAGAACTTGATGTAGTCTCGAAAGAAGGGCACGAAACTACAGGTAGTCTGAGGCAGAGTGTCCAAGAGAG
TAATGCAGCACCAAAACAGACTTCGCCGAAGGCGGCAAATGACGTGGATCGGCTGACACGGGATTTTGAC
GAGCGCATCCGCGTCCGCGGGGATGGACGTGGACTCTAA

Reference
(1) Hodges LD; Cuperus J; Ream W (2004). Agrobacterium rhizogenes GALLS protein substitutes for Agrobacterium tumefaciens single-stranded DNA-binding protein VirE2. J Bacteriol. 186(10):3065-77. [PudMed:15126468] experimental
 
experimental experimental literature