| Effector Protein ID: 10954701 |
 T4SS name [ID] | VirB [20] |
| Gene symbol | riorf55 |
| Locus tag | pRi1724_p056 |
| Strain | Agrobacterium rhizogenes plasmid pRi1724 (NC_002575) |
| Replicon | plasmid pRi1724 [GenBank: NC_002575] [Browse all T4SS(s) in this replicon] |
| Location [Strand] | 63178..68466 [-] |
| Product | hypothetical protein |
| Effector type | Protein |
| Descripton | GALLS |
| T4CP requirement | T4CP Depedent |
| Target/Effection | GALLS can substitute for VirE2 in strains of Agrobacterium rhizogenes that lack virE2. GALLS can be made in two forms: GALLS-FL (full-length) and GALLS-CT (C-terminal). On some hosts, both GALLS proteins were required to substitute for VirE2. GALLS-FL localizes to the nucleus, while GALLS-CT remains cytoplasmic. GALLS-FL also interacts with IMPa-4 and with VirD2. So GALLS may aid in stabilization and/or nuclear import of VirD2/T-strand complexes. (Hodges, et al. 2004; Hodges, et al. 2006; Hodges, et al. 2009; Gelvin, 2012) |
| UniProt ID | Q9KWC9 |
| KEGG ID | NA |
| PDB ID | NA |
| Domain hit(s) vs Pfam | AAA_30 [PF13604], Evalue: 2.4e-23, Aligned region: 146..313 UvrD_C_2 [PF13538], Evalue: 1.2e-06, Aligned region: 438..548 |
| Protein Sequence: 1762 a.a. [Download] |
| >gi|10954701|ref|NP_066636.1| hypothetical protein [Agrobacterium rhizogenes] MSDPGMAAVDTSVPMRFQTDLRQFSNLLDESNIVGWVVNHAANRNACFGQDQLKISAARALTHYKRPVQE IDLLFQQVVASDKLTRLDSPDGQPLYTSTELRAAEQNTVRHVTNLSAEESFGVPGTIVDMVADRSIFTPE LRGALHYLSRRNRASTMVGVAGSAKTSVLGALNEAVDRFNEEALPTEKIKLIAFVPTNRAAQELREKGLR DVATTFKAKDRTIGKNTIVVIDEMSMAKTQEIAQLIETVANATSYHPTERPKLICVGDDRQLPPVGPGDL LPIIMEQAGCYELVQPLRQLDARSRIETEKLGRDIRRDAKSAVGHYLTAIEQMGLVHFVYAPPGGSNVRA SDQITDRMLEEMNRVCDRYPVDDRLVMAHSNRSVNRANIALHQRFVDKSGLQDQQMSVWSSVIDSGSSES GIDEGSSRRVQQRINLVLGDRIIFSENRLQADIRNGTFATVVGLNSTEPSRAENGTSAGVRITARLDGSG RTVTWTDSEFKGFTYGYAATIYKSQGATVDHAILLCDGALSDKLTYVGLTRHRSRLDVIASPLVANNVEA LSAKLTQQSAPNNSIQFPALEPAAFQERSSVSNAHLLRPLDQMEISPTTPLSFQGRQESSFGENQLHEPS TSDVEMPESADLYPSAIDFEVFQSSVITTEEQMQPEFCGSESTANTSAHESHQVTIATVTNLGIAIEKRK RAAAKEEIDSRKKMARHSVSSAEEVEDITSLFERLKLATSVTNTVQPTYADVDHIIGTDGRVGPIPHRED DPGSNWELSSTNAPSNVSEKRSTKDSGEKNMASNDQKMQGADSAGSERHFSQTSIVEALQAGDHKKLVET LAATGSLVEKADAGSALKAMADAYERDIKADPEKVRMLYANRRVDVDALAKEIEKQGLASGRLSGDPVSF KAFNNKDIRFRVGDRLSVTSSYRNLIAGEAGRIEKIEGKSVTFKPDGSSQSKTFEASTGEPGDRGVKGYK IEGPMTVIGSAYELTRHDDGKRVDQIYLLNDAAHGINANGALLSKADERTQLFTSLKDTADRDILARQLG KPGSELYQDKFPALPDYTAAIDRIAKRQEVVAARGEEKTSTPIDLAPQTPPDAADAAVNNQKRIVEALQA GDHKKLVETLAATGSLVENTNLVSTLKAIAQTYERDISADPEKVRALYANKRDHVDLLAEEIEKQGLASG RLSGDPVSFKAFNNKDIRFRVGDRLSVTSSYRNLIAGEAGRIEKIEGKSVTFKPDGSSQSKTFEASTGEP GDRGVKGYKIEGPTTIIGSAYEIRNHDDGRRVDQVYVLHSAIFGSNAASALLSKADERTQVFTVQSDTAD RHILASQLSKPGSELYQDKFPVSPDYAAAIDRIAKREELRAGRGEESPTVQMEPVNQTPYEVGHWTPDSQ KSVVEALQAGDHKKLVETLAATGSLVEKADAGSALKAMADAYERDIKADPEKVRMLYANRRVDVDALAKE IEKQGLASGRLSGDPVSFKAFNNKDIRFRVGDRLSVTSSYRNLIAGEAGRIEKIEGKSVTFKPDGSSQSK TFEASTGEPGDRGVKGYKIEGPMTVIGSAYELTRHDDGKRVDQIYLLNDAAHGINANGALLSKADERTQL FTSLKDTADRDILARQLGKPGSELYQDKFPALPDYTAAIDRIAKRQEVVAARGEIKPIERTEAPEIPQVQ ATIVGETSIIRLPDRIKTNREKPQELDVVSKEGHETTGSLRQSVQESNAAPKQTSPKAANDVDRLTRDFD ERIRVRGDGRGL |
| - |
| Nucleotide Sequence: 5289 bp [Download] |
| >gi|10954646|ref|NC_002575.1|:c68466-63178 Agrobacterium rhizogenes plasmid pRi1724 DNA, complete sequence ATGTCCGATCCCGGAATGGCTGCTGTTGACACGTCTGTCCCTATGCGCTTCCAGACAGATCTTCGCCAGT TCAGTAACCTGCTGGACGAGAGCAATATTGTGGGATGGGTCGTAAACCACGCCGCAAATCGCAATGCGTG CTTCGGACAAGACCAACTTAAGATTTCGGCGGCTCGGGCACTCACGCACTACAAACGGCCGGTACAAGAA ATCGACCTGCTATTCCAACAGGTCGTCGCAAGCGACAAACTGACCCGGCTTGATTCTCCAGATGGCCAAC CTCTCTACACCTCTACAGAGCTGAGAGCTGCGGAACAGAATACTGTACGTCATGTCACTAATCTTTCCGC AGAAGAAAGCTTCGGCGTTCCGGGAACCATTGTTGATATGGTCGCCGATAGAAGCATTTTCACACCGGAG CTCAGAGGAGCATTACATTATCTGTCTAGGCGAAATCGAGCTTCCACTATGGTGGGCGTAGCGGGTTCCG CAAAAACAAGTGTACTTGGAGCCCTGAACGAAGCGGTGGACAGGTTCAACGAGGAAGCGCTCCCGACCGA GAAAATAAAGTTGATTGCCTTCGTACCCACCAACCGGGCGGCCCAGGAGCTTCGTGAGAAAGGTCTTCGC GACGTCGCGACGACTTTCAAGGCGAAAGACCGCACCATCGGAAAAAATACCATCGTCGTGATCGACGAAA TGTCGATGGCCAAGACGCAGGAGATCGCGCAACTAATCGAGACAGTTGCAAATGCCACTTCCTATCACCC AACCGAAAGGCCGAAACTGATATGTGTGGGAGACGATCGGCAACTGCCCCCTGTTGGACCTGGTGATCTG CTTCCGATTATTATGGAGCAAGCCGGCTGCTATGAACTCGTCCAGCCGCTTCGACAGCTCGACGCGCGGT CGCGAATAGAAACAGAAAAGCTCGGACGTGACATACGACGCGATGCCAAGAGCGCCGTCGGGCACTATCT CACAGCGATCGAACAAATGGGCCTCGTCCATTTCGTATATGCTCCTCCTGGCGGCTCAAACGTCAGGGCG TCAGACCAGATCACTGACCGCATGCTGGAGGAGATGAATAGAGTTTGCGACCGATATCCGGTGGATGATC GTCTTGTGATGGCACACTCCAACAGGAGCGTTAATCGCGCCAATATCGCTCTTCATCAGAGGTTTGTTGA CAAATCCGGATTGCAAGACCAGCAAATGAGTGTGTGGTCAAGCGTAATAGATAGTGGAAGTTCGGAATCT GGAATAGATGAGGGTTCGTCGAGACGCGTTCAGCAAAGGATTAATCTCGTCTTGGGAGACAGGATTATAT TCAGCGAAAACCGTCTGCAAGCGGACATCCGCAATGGTACTTTTGCAACTGTAGTCGGACTTAATAGCAC AGAACCTTCCCGAGCTGAGAATGGCACATCTGCTGGGGTTCGCATTACTGCAAGGCTAGACGGATCTGGA CGAACCGTCACTTGGACCGATAGCGAGTTTAAGGGTTTTACCTACGGCTATGCTGCTACAATTTATAAGA GTCAGGGGGCCACAGTTGATCACGCCATTCTCCTCTGTGATGGTGCTCTTTCAGATAAGCTGACCTATGT CGGCCTGACGAGGCATCGCAGTCGTCTCGACGTCATTGCTTCTCCCCTCGTGGCAAACAATGTTGAGGCT CTCAGTGCAAAGCTCACTCAGCAAAGCGCGCCAAATAATTCCATTCAATTCCCGGCGCTCGAACCTGCTG CCTTCCAAGAGCGCTCGTCGGTTTCGAACGCGCACTTGCTAAGGCCGCTCGACCAGATGGAAATCTCTCC AACGACGCCGCTTTCCTTCCAAGGCAGACAAGAAAGCTCCTTCGGGGAGAACCAGCTGCACGAACCATCC ACATCGGATGTGGAAATGCCGGAAAGCGCGGACCTTTACCCGAGCGCCATTGACTTCGAAGTTTTCCAGT CTTCTGTCATCACGACTGAAGAGCAAATGCAACCGGAATTCTGCGGGTCGGAATCGACGGCTAACACCTC AGCCCACGAAAGCCACCAAGTCACGATCGCAACGGTAACAAACCTGGGGATAGCGATCGAGAAACGGAAA CGAGCGGCGGCAAAGGAAGAAATCGATTCTCGCAAAAAAATGGCCCGTCACTCAGTTAGTTCTGCCGAGG AAGTTGAAGATATCACTTCCCTATTCGAACGATTGAAACTGGCAACCTCGGTCACGAACACAGTTCAACC GACGTATGCGGACGTCGATCATATCATCGGTACTGATGGCCGAGTGGGTCCAATACCGCACCGGGAAGAT GATCCGGGATCAAATTGGGAATTGTCGTCAACGAACGCCCCCTCAAACGTCTCAGAGAAACGATCGACCA AAGACTCAGGAGAAAAAAATATGGCTTCGAATGACCAGAAAATGCAAGGCGCTGACTCTGCAGGCAGCGA GCGTCATTTTTCGCAGACTTCCATTGTGGAAGCCCTGCAGGCTGGCGATCACAAGAAGCTCGTGGAAACG CTCGCCGCAACCGGCTCCCTTGTCGAGAAGGCGGACGCCGGGTCGGCGCTGAAAGCAATGGCGGATGCTT ACGAAAGGGATATCAAGGCAGATCCCGAAAAGGTCCGCATGCTCTATGCCAATCGGCGCGTTGATGTAGA TGCGCTGGCCAAGGAGATCGAAAAACAGGGCCTTGCATCCGGTCGCCTGAGTGGCGACCCTGTCAGCTTC AAGGCGTTCAACAACAAGGATATCCGTTTCAGGGTCGGCGACAGGCTCAGCGTCACCAGCAGCTACAGGA ATCTCATTGCCGGTGAAGCCGGCCGGATCGAAAAGATCGAAGGCAAATCCGTTACCTTCAAACCCGATGG CAGCAGTCAGAGCAAGACCTTCGAGGCCAGCACCGGCGAGCCGGGTGACAGGGGCGTCAAGGGCTACAAA ATCGAAGGCCCGATGACGGTCATCGGCTCCGCCTATGAACTTACGCGACATGATGACGGCAAACGTGTTG ACCAAATCTATCTCTTGAACGACGCTGCCCATGGCATCAACGCCAATGGCGCCTTGCTGAGCAAGGCCGA CGAGCGGACGCAGCTTTTCACTTCGCTCAAGGACACCGCCGACAGAGACATCTTGGCCAGGCAACTGGGA AAGCCGGGCAGCGAGCTCTACCAGGACAAATTCCCAGCCTTGCCGGACTACACAGCCGCCATCGATCGGA TTGCAAAACGCCAGGAAGTTGTCGCGGCACGTGGCGAGGAGAAAACTTCCACCCCGATAGATCTGGCGCC CCAAACGCCGCCCGACGCTGCCGATGCGGCAGTCAATAACCAGAAAAGAATCGTCGAGGCCTTGCAGGCA GGCGACCACAAGAAGCTTGTGGAAACGCTAGCCGCAACCGGCTCCCTTGTTGAGAACACAAATCTCGTAT CAACGTTGAAAGCGATAGCGCAGACTTATGAAAGAGACATTAGTGCGGACCCCGAGAAAGTCCGCGCTCT CTACGCCAACAAACGTGATCATGTAGATTTGCTTGCCGAAGAGATCGAAAAGCAGGGCCTTGCATCCGGT CGCCTGAGTGGCGACCCTGTCAGCTTCAAGGCGTTTAACAACAAGGATATCCGTTTCAGGGTCGGTGACA GGCTCAGCGTCACCAGCAGCTATAGGAATCTCATTGCCGGTGAAGCCGGCCGGATCGAAAAGATCGAAGG CAAATCCGTTACCTTCAAACCCGATGGCAGCAGTCAGAGCAAGACCTTCGAGGCCAGCACCGGCGAGCCG GGTGACAGGGGTGTCAAGGGCTATAAGATTGAGGGGCCCACGACAATCATCGGCTCCGCCTACGAAATCA GAAATCACGACGATGGCAGGCGCGTCGATCAGGTTTATGTCTTGCACAGTGCCATTTTCGGCTCAAATGC CGCGAGCGCCCTGTTGAGCAAGGCCGACGAACGAACCCAGGTTTTCACCGTGCAAAGCGACACGGCTGAC AGACACATCTTGGCCAGCCAACTAAGCAAGCCTGGCAGCGAGCTCTATCAGGACAAATTCCCAGTCTCGC CGGACTACGCAGCCGCGATCGACCGGATCGCGAAACGCGAGGAACTTAGGGCCGGACGTGGCGAGGAAAG TCCTACTGTTCAAATGGAGCCGGTGAACCAAACGCCCTACGAGGTGGGACATTGGACGCCTGACAGTCAA AAATCTGTGGTGGAAGCCCTGCAGGCTGGCGATCACAAGAAGCTCGTGGAAACGCTCGCCGCAACCGGCT CCCTTGTCGAGAAGGCGGACGCCGGGTCGGCGCTGAAAGCAATGGCGGATGCTTACGAAAGGGATATCAA GGCAGATCCCGAAAAGGTCCGCATGCTCTATGCCAATCGGCGCGTTGATGTAGATGCGCTGGCCAAGGAG ATCGAAAAACAGGGCCTTGCATCCGGTCGCCTGAGTGGCGACCCTGTCAGCTTCAAGGCGTTCAACAACA AGGATATCCGTTTCAGGGTCGGCGACAGGCTCAGCGTCACCAGCAGCTACAGGAATCTCATTGCCGGTGA AGCCGGCCGGATCGAAAAGATCGAAGGCAAATCCGTTACCTTCAAACCCGATGGCAGCAGTCAGAGCAAG ACCTTCGAGGCCAGCACCGGCGAGCCGGGTGACAGGGGCGTCAAGGGCTACAAAATCGAAGGCCCGATGA CGGTCATCGGCTCCGCCTATGAACTTACGCGACATGATGACGGCAAACGTGTTGACCAAATCTATCTCTT GAACGACGCTGCCCATGGCATCAACGCCAATGGCGCCTTGCTGAGCAAGGCCGACGAGCGGACGCAGCTT TTCACTTCGCTCAAGGACACCGCCGACAGAGACATCTTGGCCAGGCAACTGGGAAAGCCGGGCAGCGAGC TCTACCAGGACAAATTCCCAGCCTTGCCGGACTACACAGCCGCCATCGATCGGATTGCAAAACGCCAGGA AGTTGTCGCGGCACGTGGCGAGATCAAGCCGATTGAGCGCACCGAAGCGCCCGAAATACCCCAGGTTCAA GCTACTATCGTTGGCGAGACCTCGATCATTCGGTTGCCCGATCGGATAAAAACCAATCGCGAGAAGCCCC AAGAACTTGATGTAGTCTCGAAAGAAGGGCACGAAACTACAGGTAGTCTGAGGCAGAGTGTCCAAGAGAG TAATGCAGCACCAAAACAGACTTCGCCGAAGGCGGCAAATGACGTGGATCGGCTGACACGGGATTTTGAC GAGCGCATCCGCGTCCGCGGGGATGGACGTGGACTCTAA |
| Reference |
(1) Hodges LD; Cuperus J; Ream W (2004). Agrobacterium rhizogenes GALLS protein substitutes for Agrobacterium tumefaciens single-stranded DNA-binding protein VirE2. J Bacteriol. 186(10):3065-77. [PudMed:15126468] 
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| experimental literature |