 T4SS ID | 638 |
| Strain | Clostridium perfringens plasmid pCW3 (NC_010937) |
| Replicon | plasmid pCW3 [Browse all T4SS(s) in this replicon] |
| Accession | NC_010937 |
| Location | 25890..36479 |
| Name | Tcp |
| Function | conjugation |
| Classification | Type IVD; Type GP |
 Experimental investigation has been performed on this T4SS. | |
T4SS components |
| Component | TcpA | TcpC | TcpD | TcpE | TcpF | TcpG | TcpH |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
The information of T4SS components from NC_010937 | ||||||
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| # | Locus tag (Gene) | Coordinates [+/-], size (bp) | Protein GI | Product | Component | |
| 1 | pCW3_0021 | 21606..21734 [+], 129 | 190015757 | hypothetical protein | ||
| 2 | pCW3_0022 | 21750..22007 [+], 258 | 190015758 | hypothetical protein | ||
| 3 | pCW3_0023 (srtA) | 22017..22661 [+], 645 | 190015759 | probable sortase surface protein transpeptidase | ||
| 4 | pCW3_0024 | 22672..22974 [+], 303 | 190015760 | hypothetical protein | ||
| 5 | pCW3_0025 | 22978..23196 [+], 219 | 190015761 | hypothetical protein | ||
| 6 | pCW3_0026 (dam) | 23260..24024 [+], 765 | 190015762 | probable site-specific DNA-methyltransferase | ||
| 7 | pCW3_0027 | 24051..24335 [+], 285 | 190015763 | hypothetical protein | ||
| 8 | pCW3_0028 | 24336..24644 [+], 309 | 190015764 | hypothetical protein | ||
| 9 | pCW3_0029 (intP) | 25036..25836 [+], 801 | 190015765 | tyrosine site-specific recombinase | ||
| 10 | pCW3_0030 (tcpA) | 25890..27482 [+], 1593 | 190015766 | probable DNA translocase coupling protein | TcpA | |
| 11 | pCW3_0031 (tcpB) | 27497..28480 [+], 984 | 190015767 | possible DNA segregation Ftsk/SpoIIE like ATPase | ||
| 12 | pCW3_0032 (tcpC) | 28473..29552 [+], 1080 | 190015768 | ORF13-like protein | TcpC | |
| 13 | pCW3_0033 (tcpD) | 29564..29911 [+], 348 | 190015769 | hypothetical protein | TcpD | |
| 14 | pCW3_0034 (tcpE) | 29923..30291 [+], 369 | 190015770 | ORF17-like protein | TcpE | |
| 15 | pCW3_0035 (tcpF) | 30349..32874 [+], 2526 | 190015771 | ORF16-like conjugation protein from Tn916-like elements | TcpF | |
| 16 | pCW3_0036 (tcpG) | 32874..33878 [+], 1005 | 190015772 | probable cell wall associated hydrolase | TcpG | |
| 17 | pCW3_0037 (tcpH) | 33981..36479 [+], 2499 | 190015773 | probable pore-forming membrane protein | TcpH | |
| 18 | pCW3_0038 (tcpI) | 36482..36976 [+], 495 | 190015774 | probable membrane-bound metal-dependent hydrolase | ||
| 19 | pCW3_0039 (tcpJ) | 36985..37566 [+], 582 | 190015775 | hypothetical protein | ||
| 20 | pCW3_0040 | 38691..38996 [+], 306 | 190015776 | hypothetical protein | ||
| 21 | pCW3_0041 (dcm) | 39011..40030 [+], 1020 | 190015777 | probable site-specific DNA-methyltransferase | ||
| 22 | pCW3_0042 | 40047..40220 [+], 174 | 190015778 | hypothetical protein | ||
| 23 | pCW3_0043 | 40217..41239 [+], 1023 | 190015779 | hypothetical protein | ||
 Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any. |
Download FASTA format files |
| Proteins Genes |
| (1) Bantwal R; Bannam TL; Porter CJ; Quinsey NS; Lyras D; Adams V; Rood JI (2012). The peptidoglycan hydrolase TcpG is required for efficient conjugative transfer of pCW3 in Clostridium perfringens. Plasmid. 67(2):139-47. [PudMed:22244927] |
| (2) Porter CJ; Bantwal R; Bannam TL; Rosado CJ; Pearce MC; Adams V; Lyras D; Whisstock JC; Rood JI (2012). The conjugation protein TcpC from Clostridium perfringens is structurally related to the type IV secretion system protein VirB8 from Gram-negative bacteria. Mol Microbiol. 83(2):275-88. [PudMed:22150951] |
| (3) Steen JA; Bannam TL; Teng WL; Devenish RJ; Rood JI (2009). The putative coupling protein TcpA interacts with other pCW3-encoded proteins to form an essential part of the conjugation complex. J Bacteriol. 191(9):2926-33. [PudMed:19251842] |
| (4) Teng WL; Bannam TL; Parsons JA; Rood JI (2008). Functional characterization and localization of the TcpH conjugation protein from Clostridium perfringens. J Bacteriol. 190(14):5075-86. [PudMed:18487333] |
| (5) Parsons JA; Bannam TL; Devenish RJ; Rood JI (2007). TcpA, an FtsK/SpoIIIE homolog, is essential for transfer of the conjugative plasmid pCW3 in Clostridium perfringens. J Bacteriol. 189(21):7782-90. [PudMed:17720795] |
| (6) Bannam TL; Teng WL; Bulach D; Lyras D; Rood JI (2006). Functional identification of conjugation and replication regions of the tetracycline resistance plasmid pCW3 from Clostridium perfringens. J Bacteriol. 188(13):4942-51. [PudMed:16788202] |