SecReT4
T4SS ID638
StrainClostridium perfringens plasmid pCW3 (NC_010937)
Repliconplasmid pCW3 [Browse all T4SS(s) in this replicon]
AccessionNC_010937
Location25890..36479
NameTcp
Functionconjugation
ClassificationType IVD; Type GP
experimental Experimental investigation has been performed on this T4SS.

T4SS components
ComponentTcpATcpCTcpDTcpETcpFTcpGTcpH
Number1111111

The information of T4SS components from NC_010937
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1pCW3_002121606..21734 [+], 129190015757hypothetical protein 
2pCW3_002221750..22007 [+], 258190015758hypothetical protein 
3pCW3_0023 (srtA)22017..22661 [+], 645190015759probable sortase surface protein transpeptidase 
4pCW3_002422672..22974 [+], 303190015760hypothetical protein 
5pCW3_002522978..23196 [+], 219190015761hypothetical protein 
6pCW3_0026 (dam)23260..24024 [+], 765190015762probable site-specific DNA-methyltransferase 
7pCW3_002724051..24335 [+], 285190015763hypothetical protein 
8pCW3_002824336..24644 [+], 309190015764hypothetical protein 
9pCW3_0029 (intP)25036..25836 [+], 801190015765tyrosine site-specific recombinase 
10pCW3_0030 (tcpA)25890..27482 [+], 1593190015766probable DNA translocase coupling protein  TcpA
11pCW3_0031 (tcpB)27497..28480 [+], 984190015767possible DNA segregation Ftsk/SpoIIE like ATPase 
12pCW3_0032 (tcpC)28473..29552 [+], 1080190015768ORF13-like protein  TcpC
13pCW3_0033 (tcpD)29564..29911 [+], 348190015769hypothetical protein  TcpD
14pCW3_0034 (tcpE)29923..30291 [+], 369190015770ORF17-like protein  TcpE
15pCW3_0035 (tcpF)30349..32874 [+], 2526190015771ORF16-like conjugation protein from Tn916-like elements  TcpF
16pCW3_0036 (tcpG)32874..33878 [+], 1005190015772probable cell wall associated hydrolase  TcpG
17pCW3_0037 (tcpH)33981..36479 [+], 2499190015773probable pore-forming membrane protein  TcpH
18pCW3_0038 (tcpI)36482..36976 [+], 495190015774probable membrane-bound metal-dependent hydrolase 
19pCW3_0039 (tcpJ)36985..37566 [+], 582190015775hypothetical protein 
20pCW3_004038691..38996 [+], 306190015776hypothetical protein 
21pCW3_0041 (dcm)39011..40030 [+], 1020190015777probable site-specific DNA-methyltransferase 
22pCW3_004240047..40220 [+], 174190015778hypothetical protein 
23pCW3_004340217..41239 [+], 1023190015779hypothetical protein 
 
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes
(1) Bantwal R; Bannam TL; Porter CJ; Quinsey NS; Lyras D; Adams V; Rood JI (2012). The peptidoglycan hydrolase TcpG is required for efficient conjugative transfer of pCW3 in Clostridium perfringens. Plasmid. 67(2):139-47. [PudMed:22244927]
(2) Porter CJ; Bantwal R; Bannam TL; Rosado CJ; Pearce MC; Adams V; Lyras D; Whisstock JC; Rood JI (2012). The conjugation protein TcpC from Clostridium perfringens is structurally related to the type IV secretion system protein VirB8 from Gram-negative bacteria. Mol Microbiol. 83(2):275-88. [PudMed:22150951]
(3) Steen JA; Bannam TL; Teng WL; Devenish RJ; Rood JI (2009). The putative coupling protein TcpA interacts with other pCW3-encoded proteins to form an essential part of the conjugation complex. J Bacteriol. 191(9):2926-33. [PudMed:19251842]
(4) Teng WL; Bannam TL; Parsons JA; Rood JI (2008). Functional characterization and localization of the TcpH conjugation protein from Clostridium perfringens. J Bacteriol. 190(14):5075-86. [PudMed:18487333]
(5) Parsons JA; Bannam TL; Devenish RJ; Rood JI (2007). TcpA, an FtsK/SpoIIIE homolog, is essential for transfer of the conjugative plasmid pCW3 in Clostridium perfringens. J Bacteriol. 189(21):7782-90. [PudMed:17720795]
(6) Bannam TL; Teng WL; Bulach D; Lyras D; Rood JI (2006). Functional identification of conjugation and replication regions of the tetracycline resistance plasmid pCW3 from Clostridium perfringens. J Bacteriol. 188(13):4942-51. [PudMed:16788202]
 

Genomics & Bioinformatics Group in MML, SJTU