T4SS ID	557
Strain	Neisseria gonorrhoeae NCCP11945
Replicon	chromosome [Browse all T4SS(s) in this replicon]
Accession	NC_011035
Location	866326..886920
Name	Tra_F
Function	DNA release
Classification	Type IVA; Type F
This T4SS has been mentioned in a genome sequencing report.

T4SS components

Component	TraA_F	TraB_F	TraC_F	TraD_F	TraE_F	TraF_F	TraG_F	TraH_F	TraI_F
Number	1	1	1	1	1	1	1	1	0
Component	TraK_F	TraL_F	TraM_F	TraN_F	TraQ_F	TraU_F	TraV_F	TraW_F	TraX_F
Number	1	1	0	1	0	1	1	1	0
Component	TraY_F	TrbC_F	Orf169_F
Number	0	1	1

The information of T4SS components from NC_011035
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	NGK_1058	861328..861639 [+], 312	194098621	hypothetical protein
2	NGK_1059	861911..862090 [+], 180	194098622	hypothetical protein
3	NGK_1060	862205..863275 [+], 1071	194098623	hypothetical protein
4	NGK_1061	863328..863942 [+], 615	194098624	hypothetical protein
5	NGK_1062	863997..864359 [+], 363	194098625	hypothetical protein
6	NGK_1063	864563..865150 [+], 588	194098626	CspA protein
7	NGK_1064	865275..865520 [+], 246	194098627	hypothetical protein
8	NGK_1065	865577..865735 [-], 159	194098628	hypothetical protein
9	NGK_1066	865739..865996 [-], 258	194098629	AtlA protein
10	NGK_1067	866326..869328 [-], 3003	194098630	protein TraG	TraG_F
11	NGK_1068	869335..870693 [-], 1359	194098631	protein TraH	TraH_F
12	NGK_1069	871018..871794 [-], 777	194098632	protein TraF	TraF_F
13	NGK_1070	871806..872231 [-], 426	194098633	hypothetical protein
14	NGK_1071	872218..873471 [-], 1254	194098634	protein TraN	TraN_F
15	NGK_1072	873798..875042 [-], 1245	194098635	hypothetical protein
16	NGK_1073	875039..875794 [-], 756	194098636	protein TrbC	TrbC_F
17	NGK_1074	875806..876924 [-], 1119	194098637	protein TraU	TraU_F
18	NGK_1075	876921..877577 [-], 657	194098638	protein TraW	TraW_F
19	NGK_1076	877586..878131 [-], 546	194098639	protein TrbI
20	NGK_1077	878131..878679 [-], 549	194098640	hypothetical protein
21	NGK_1078	878707..881283 [-], 2577	194098641	protein TraC	TraC_F
22	NGK_1079	881293..881898 [-], 606	194098642	protein TraV	TraV_F
23	NGK_1080	881871..882431 [-], 561	194098643	protein DsbC
24	NGK_1081	882600..883913 [-], 1314	194098644	protein TraB	TraB_F
25	NGK_1082	883915..884472 [-], 558	194098645	protein TraK	TraK_F
26	NGK_1083	884651..885301 [-], 651	194098646	protein TraE	TraE_F
27	NGK_1084	885313..885594 [-], 282	194098647	protein TraL	TraL_F
28	NGK_1085	885616..885840 [-], 225	194098648	TraA protein	TraA_F
29	NGK_1086	885968..886489 [-], 522	194098649	hypothetical protein
30	NGK_1087	886459..886920 [-], 462	194098650	protein LtgX	Orf169_F
31	NGK_1088	887431..887850 [+], 420	194098651	hypothetical protein
32	NGK_1089	887847..890399 [+], 2553	194098652	TraI

 This T4SS contains information of accessory protein.

Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files

Proteins        Genes

The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#	Accessory protein(GI)	motif(s)	Substrate(s)	Function	Reference
1	ParA (193933883)	ND	TraI	The N. gonorrhoeae ParA and ParB coordinate early DNA substrate docking reactions with the cognate GGI-encoded T4SSs.	(1) PubMed: 15897178
2	ParB (193933884)	ND	TraI	The N. gonorrhoeae ParA and ParB coordinate early DNA substrate docking reactions with the cognate GGI-encoded T4SSs.	(2) PubMed: 26104562

Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Leonard TA et al. (2005). Towards understanding the molecular basis of bacterial DNA segregation. Philos Trans R Soc Lond B Biol Sci. 360(1455):523-35. [PudMed:15897178]

(2) Obergfell KP et al. (2015). Mobile DNA in the Pathogenic Neisseria. Microbiol Spectr. 3(1):MDNA3-0015-2014. [PudMed:26104562]

(1) Chung GT; Yoo JS; Oh HB; Lee YS; Cha SH; Kim SJ; Yoo CK (2008). Complete genome sequence of Neisseria gonorrhoeae NCCP11945. J Bacteriol. 190(17):6035-6. [PudMed:18586945]