T4SS ID557
StrainNeisseria gonorrhoeae NCCP11945
Repliconchromosome [Browse all T4SS(s) in this replicon]
FunctionDNA release
ClassificationType IVA; Type F
sequence This T4SS has been mentioned in a genome sequencing report.

T4SS components

The information of T4SS components from NC_011035
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1NGK_1058861328..861639 [+], 312194098621hypothetical protein 
2NGK_1059861911..862090 [+], 180194098622hypothetical protein 
3NGK_1060862205..863275 [+], 1071194098623hypothetical protein 
4NGK_1061863328..863942 [+], 615194098624hypothetical protein 
5NGK_1062863997..864359 [+], 363194098625hypothetical protein 
6NGK_1063864563..865150 [+], 588194098626CspA protein 
7NGK_1064865275..865520 [+], 246194098627hypothetical protein 
8NGK_1065865577..865735 [-], 159194098628hypothetical protein 
9NGK_1066865739..865996 [-], 258194098629AtlA protein 
10NGK_1067866326..869328 [-], 3003194098630protein TraG  TraG_F
11NGK_1068869335..870693 [-], 1359194098631protein TraH  TraH_F
12NGK_1069871018..871794 [-], 777194098632protein TraF  TraF_F
13NGK_1070871806..872231 [-], 426194098633hypothetical protein 
14NGK_1071872218..873471 [-], 1254194098634protein TraN  TraN_F
15NGK_1072873798..875042 [-], 1245194098635hypothetical protein 
16NGK_1073875039..875794 [-], 756194098636protein TrbC  TrbC_F
17NGK_1074875806..876924 [-], 1119194098637protein TraU  TraU_F
18NGK_1075876921..877577 [-], 657194098638protein TraW  TraW_F
19NGK_1076877586..878131 [-], 546194098639protein TrbI 
20NGK_1077878131..878679 [-], 549194098640hypothetical protein 
21NGK_1078878707..881283 [-], 2577194098641protein TraC  TraC_F
22NGK_1079881293..881898 [-], 606194098642protein TraV  TraV_F
23NGK_1080881871..882431 [-], 561194098643protein DsbC 
24NGK_1081882600..883913 [-], 1314194098644protein TraB  TraB_F
25NGK_1082883915..884472 [-], 558194098645protein TraK  TraK_F
26NGK_1083884651..885301 [-], 651194098646protein TraE  TraE_F
27NGK_1084885313..885594 [-], 282194098647protein TraL  TraL_F
28NGK_1085885616..885840 [-], 225194098648TraA protein  TraA_F
29NGK_1086885968..886489 [-], 522194098649hypothetical protein 
30NGK_1087886459..886920 [-], 462194098650protein LtgX  Orf169_F
31NGK_1088887431..887850 [+], 420194098651hypothetical protein 
32NGK_1089887847..890399 [+], 2553194098652TraI 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1ParA (193933883)NDTraIThe N. gonorrhoeae ParA and ParB coordinate early DNA substrate docking reactions with the cognate GGI-encoded T4SSs.(1) PubMed: 15897178
2ParB (193933884)NDTraIThe N. gonorrhoeae ParA and ParB coordinate early DNA substrate docking reactions with the cognate GGI-encoded T4SSs.(2) PubMed: 26104562
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Leonard TA et al. (2005). Towards understanding the molecular basis of bacterial DNA segregation. Philos Trans R Soc Lond B Biol Sci. 360(1455):523-35. [PudMed:15897178] experimental
(2) Obergfell KP et al. (2015). Mobile DNA in the Pathogenic Neisseria. Microbiol Spectr. 3(1):MDNA3-0015-2014. [PudMed:26104562] experimental
(1) Chung GT; Yoo JS; Oh HB; Lee YS; Cha SH; Kim SJ; Yoo CK (2008). Complete genome sequence of Neisseria gonorrhoeae NCCP11945. J Bacteriol. 190(17):6035-6. [PudMed:18586945]