Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   V8V52_RS07150 Genome accession   NZ_CP150648
Coordinates   1395923..1398244 (+) Length   773 a.a.
NCBI ID   WP_088034190.1    Uniprot ID   A0A243A376
Organism   Bacillus mycoides strain 4BM1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1390923..1403244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8V52_RS07135 (V8V52_07135) - 1391827..1392111 (+) 285 WP_002015311.1 post-transcriptional regulator -
  V8V52_RS07140 (V8V52_07140) secDF 1392394..1394658 (+) 2265 WP_088034186.1 protein translocase subunit SecDF -
  V8V52_RS07145 (V8V52_07145) - 1394928..1395821 (+) 894 WP_088034188.1 cation diffusion facilitator family transporter -
  V8V52_RS07150 (V8V52_07150) recJ 1395923..1398244 (+) 2322 WP_088034190.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  V8V52_RS07155 (V8V52_07155) - 1398310..1398822 (+) 513 WP_002015305.1 adenine phosphoribosyltransferase -
  V8V52_RS07160 (V8V52_07160) relA 1399034..1401217 (+) 2184 WP_002015304.1 GTP diphosphokinase -
  V8V52_RS07165 (V8V52_07165) - 1401235..1401675 (+) 441 WP_002015302.1 D-tyrosyl-tRNA(Tyr) deacylase -
  V8V52_RS07170 (V8V52_07170) - 1401944..1402120 (+) 177 WP_002015300.1 hypothetical protein -

Sequence

Protein


Download         Length: 773 a.a.        Molecular weight: 87304.79 Da        Isoelectric Point: 4.9265

>NTDB_id=970917 V8V52_RS07150 WP_088034190.1 1395923..1398244(+) (recJ) [Bacillus mycoides strain 4BM1]
MLQPKTRWKEKEYNEELVSELANKLQLSPLVTSLFLGRGLNTEDKIVDFLNTADQEFHDPFLLEGMDRTVERVKKAIENG
EQILIFGDYDADGVSSTTVLYLALKELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLEGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VQRGLKHMRMTRNIGLKALFKVANVSQSEITEESIGFALAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDPHKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLNMNDVDELRRRLNEQADVILTAEDFIPITAVDAFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDADLESIRAIGSDGSHLKMALRDGQATLDTIGFGFGTYAKEISPVAKVSVVGEVSINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANLAELPKEKITMVYFSEEVLNKFSLEGYKEQLVHASEVVHFDDQYIVLLDLPKGTDELGD
VFKAGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLIQKTPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHIQLEKELVYSTYQQLYTWFETIRDHK

Nucleotide


Download         Length: 2322 bp        

>NTDB_id=970917 V8V52_RS07150 WP_088034190.1 1395923..1398244(+) (recJ) [Bacillus mycoides strain 4BM1]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGAGGAACTAGTGAGTGAATTAGCAAATAAATTGCAACT
ATCACCTCTTGTGACTTCATTATTTCTTGGCAGAGGCTTAAATACAGAAGATAAGATTGTAGACTTTTTAAATACAGCAG
ATCAAGAATTTCATGATCCATTTTTACTTGAAGGAATGGATCGGACTGTAGAACGTGTGAAAAAAGCTATTGAAAATGGA
GAACAAATATTAATATTTGGCGATTACGATGCGGACGGAGTAAGTAGTACGACTGTGTTATATCTTGCTCTCAAAGAATT
AGGTGCAGAAGTAGAATTTTATATTCCAAATCGTTTTACTGAAGGCTATGGGCCAAATGAAGAAGCGTTTCGTTGGGCGC
ACAGTGCAGGGTTCTCACTAATTATTACTGTCGATACTGGTATCGCAGCGGTACATGAAGCGAAGGTAGCGAAGGAACTT
GGAATAGATCTTATTATTACAGATCATCATGAGCCACCACCAGAATTACCAGAGGCACTTGCGATTATTCACCCGAAATT
AGAAGGCGGTGTTTATCCGTTTCATTATTTAGCGGGTGTTGGTGTTGCATTTAAAGTTGCACATGCACTTTTGGGCCGTG
TGCCAGAGCATTTGTTGGAAATTGCAGTAATCGGTACGGTAGCCGATTTAGTATCGCTTCACGGTGAAAATAGATTATTA
GTACAGCGTGGACTGAAGCATATGCGAATGACTCGAAATATCGGTTTAAAGGCGTTATTTAAAGTCGCTAACGTTTCGCA
AAGCGAAATTACAGAAGAAAGCATCGGGTTCGCACTCGCACCTCGTATTAATGCAGTTGGTCGTTTGGAAGATGCAACAC
CTGCTGTACATCTTTTATTATCAGAAGATCCAGAGGAAGCGAAAGAGCTAGCTGAAGAAATTGATGAGCTGAACAAACTT
CGAAAAGATATTGTAAAGCAAATTACTGAAGAAGCTATCGCTGAAGTCGAAAATAATTTCCCGCCAGAAGAAAATAAAGT
GTTAGTCCTTGCAAAAGAAGGTTGGAATCCAGGGGTAATTGGAATTGTCGCTTCTAAGTTAGTTGAACGTTTTTATCGTC
CGACGATTGTATTAAGCATTGATCCTCATAAAGAAACAGCAAAGGGATCGGCTCGTAGTATTGCAGGATTTGATTTGTTT
GCAAACCTTTCAGATTGCCGTGAATTGTTACCGCATTTCGGAGGTCATCCGATGGCAGCGGGAATGACACTTAATATGAA
TGATGTGGATGAATTAAGACGCAGGTTAAATGAGCAAGCAGATGTAATTTTAACAGCAGAAGATTTTATTCCGATTACAG
CAGTGGATGCATTTTGTAAAGTAGAAGATGTAACGCTTGCCGCGATTGAGGATATGCAAAAGTTAGCGCCATTTGGCGTA
GGAAATCCGAAACCGCGTATTGCGGTGAAAGATGCCGACTTAGAAAGTATCCGTGCAATTGGATCTGATGGTTCGCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGACACGATTGGATTTGGATTTGGTACTTATGCGAAAGAAATCTCTC
CAGTTGCGAAGGTTTCTGTAGTAGGAGAAGTGTCCATTAATGAATGGAATAACTTTAAAAAGCCGCAATTAATGGTGCAA
GATATTGCTGTAGAGGCATGGCAATTATTTGACTGGCGTAGTATGCGTAATGTAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACAATGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAAGGCTATAAAGAACAGCTCGTGC
ATGCATCAGAGGTAGTTCATTTTGATGATCAATACATTGTGCTACTTGATTTGCCGAAAGGAACAGATGAATTAGGTGAT
GTATTTAAGGCTGGATTCCCAGCTCGAATTTACACGCTATTTTATCAAGAGAATAATCATTTATTTAGTACGGTCCCAAC
GAGGGAACATTTTAAATGGTACTATTCTTTCTTAATCCAAAAAACACCATTTTCTTTAAGGCAATATGGGGAACAACTGT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GACGGTGTCATTTTTATGGCAGATAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CATACAATTAGAGAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTGATCATAAAT
AA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)

DHHA1 domain (DHHA1)

(315-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A243A376

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.645

100

0.528

Multiple sequence alignment