Detailed information NTG0961868

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: WKU69_RS04655
• Genome accession: NZ_CP149838
• Coordinates: 951665..952783 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain 2024CK-00130
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 946665..957783

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WKU69_RS04625 (WKU69_04625)	-	947494..948342 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
WKU69_RS04630 (WKU69_04630)	-	948595..949050 (+)	456	WP_000782976.1	bacteriohemerythrin	-
WKU69_RS04635 (WKU69_04635)	-	949203..950270 (+)	1068	WP_029777899.1	membrane protein	-
WKU69_RS04640 (WKU69_04640)	-	950270..950593 (+)	324	WP_000401389.1	RnfH family protein	-
WKU69_RS04645 (WKU69_04645)	bamE	950648..951046 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
WKU69_RS04650 (WKU69_04650)	fur	951158..951595 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
WKU69_RS04655 (WKU69_04655)	pilU	951665..952783 (-)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
WKU69_RS04660 (WKU69_04660)	pilT	952811..953848 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
WKU69_RS04665 (WKU69_04665)	-	953975..954667 (+)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=961868 WKU69_RS04655 WP_000347034.1 951665..952783(-) (pilU) [Acinetobacter baumannii strain 2024CK-00130]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=961868 WKU69_RS04655 WP_000347034.1 951665..952783(-) (pilU) [Acinetobacter baumannii strain 2024CK-00130]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACTAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39